Biodegradable micelles with sheddable poly(ethylene glycol) shells for triggered intracellular release of doxorubicin

Sun, Huanli; Guo, Bingnan; Cheng, Ru; Meng, Fenghua; Liu, Haiyan; Zhong, Zhiyuan

doi:10.1016/j.biomaterials.2009.07.051

Cited by 422 publications

(377 citation statements)

References 43 publications

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“…44 The total amount of non-aggregated PEG-PCL-4 molecules in the final preparation was 0.45 mg, based on the volume of aqueous phase in the final preparation of 25 ml and the above value of CMC. The concentration of PEG-PCL-4 in the organic phase was 1.0 mg ml -1 and thus, the total amount of PEG-PCL-4 incorporated in the micelles was 4.55 mg.…”

Section: Copolymer Molecular Weight Mw the Results Inmentioning

confidence: 99%

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Laouini

Koutroumanis

Charcosset

et al. 2013

ACS Appl. Mater. Interfaces

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A novel membrane contactor method was used to produce size-controlled poly(ethylene glycol)-b-polycaprolactone (PEG-PCL) copolymer micelles composed of diblock copolymers with different average molecular weights, M n (9200 or 10 400 Da) and hydrophilic fractions, f (0.67 or 0.59). By injecting 570 L m −2 h −1 of the organic phase (a 1 mg mL −1 solution of PEG-PCL in tetrahydrofuran) through a microengineered nickel membrane with a hexagonal pore array and 200 μm pore spacing into deionized water agitated at 700 rpm, the micelle size linearly increased from 92 nm for a 5-μm pore size to 165 nm for a 40-μm pore size. The micelle size was finely tuned by the agitation rate, transmembrane flux and aqueous to organic phase ratio. An encapsulation efficiency of 89% and a drug loading of ∼75% (w/w) were achieved when a hydrophobic drug (vitamin E) was entrapped within the micelles, as determined by ultracentrifugation method. The drug-loaded micelles had a mean size of 146 ± 7 nm, a polydispersity index of 0.09 ± 0.01, and a ζ potential of −19.5 ± 0.2 mV. When drug-loaded micelles where stored for 50 h, a pH sensitive drug release was achieved and a maximum amount of vitamin E (23%) was released at the pH of 1.9. When a pH-sensitive hydrazone bond was incorporated between PEG and PCL blocks, no significant change in micelle size was observed at the same micellization conditions.

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Section: Copolymer Molecular Weight Mw the Results Inmentioning

confidence: 99%

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Laouini

Koutroumanis

Charcosset

et al. 2013

ACS Appl. Mater. Interfaces

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“…The critical micelle concentration (CMC) was determined by fluorescence measurement using pyrene as the fluorescent probe at 25°C. 24,25 The concentration of block copolymer was varied from 1 × 10 −6 to 0.1 mg/mL. Briefly, aliquots of pyrene solution (10 µM) were added into containers and dried with nitrogen.…”

Section: Characterizationmentioning

confidence: 99%

Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro

Qian¹

2012

IJN

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Background:In this paper, a series of amphiphilic triblock copolymers based on polyethylene glycol-poly ε-caprolactone-polyethylenimine (mPEG-PCL-g-PEI) were successfully synthesized, and their application for codelivery of chemotherapeutic drugs and DNA simultaneously was investigated. Methods and results: These copolymers could self-assemble into micelles with positive charges. The size and zeta potential of the micelles was measured, and the results indicate that temperature had a large effect on the micelles obtained. In vitro gene transfection evaluation in cancer cells indicated that the self-assembled micelles could serve as potential gene delivery vectors. In addition, hydrophobic drug entrapment efficiency and codelivery with the gene was also studied in vitro. The self-assembled micelles could load doxorubicin efficiently and increase cellular uptake in vitro, while maintaining high gene transfection efficiency. Conclusion:The triblock copolymer mPEG-PCL-g-PEI could be a novel vector for codelivery of drug and gene therapy.

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“…Similar fast release found at the initial 4 hours might be ascribed to the drug located in the hydrophilic shell or adhered to the surface of the NPs. 54 Then the remaining DOX, probably loaded deep in the hydrophobic cores, presented a different release profile in different DTT concentrations. In the presence of 10 μM DTT, the less-efficient release during the following 44 hours suggested that the insoluble DOX was difficult to be released from the inner core as reported before, 55 which met the demand that the integrity of vehicle was maintained well and the loss of drug minimized before reaching the target cells.…”

mentioning

confidence: 99%

GE11 peptide modified and reduction-responsive hyaluronic acid-based nanoparticles induced higher efficacy of doxorubicin for breast carcinoma therapy

Hu¹,

Mezghrani²,

Zhang³

et al. 2016

IJN

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Novel breast carcinoma dual-targeted redox-responsive nanoparticles (NPs) based on cholesteryl-hyaluronic acid conjugates were designed for intracellular delivery of the antitumor drug doxorubicin (DOX). A series of reduction-responsive hyaluronic acid derivatives grafted with hydrophobic cholesteryl moiety (HA-ss-Chol) and GE11 peptide conjugated HA-ss-Chol (GE11–HA-ss-Chol) were synthesized. The obtained conjugates showed attractive self-assembly characteristics and high drug loading capacity. GE11–HA-ss-Chol NPs were highly stable under conditions mimicking normal physiological conditions, while showing a fast degradation of the vehicle’s structure and accelerating the drug release dramatically in the presence of intracellular reductive environment. Furthermore, the cellular uptake assay confirmed GE11–HA-ss-Chol NPs were taken up by MDA-MB-231 cells through CD44- and epidermal growth factor receptor-mediated endocytosis. The internalization pathways of GE11–HA-ss-Chol NPs might involve clathrin-mediated endocytosis and macropinocytosis. The intracellular distribution of DOX in GE11–HA-ss-Chol NPs showed a faster release and more efficient nuclear delivery than the insensitive control. Enhanced in vitro cytotoxicity of GE11–HA-ss-Chol DOX-NPs further confirmed the superiority of their dual-targeting and redox-responsive capacity. Moreover, in vivo imaging investigation in MDA-MB-231 tumor-bearing mice confirmed that GE11–HA-ss-Chol NPs labeled with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide, a near-infrared fluorescence dye, possessed a preferable tumor accumulation ability as compared to the single-targeting counterpart (HA-ss-Chol NPs). The antitumor efficacy showed an improved therapy efficacy and lower systemic side effect. These results suggest GE11–HA-ss-Chol NPs provide a good potential platform for antitumor drugs.

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Biodegradable micelles with sheddable poly(ethylene glycol) shells for triggered intracellular release of doxorubicin

Cited by 422 publications

References 43 publications

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro

GE11 peptide modified and reduction-responsive hyaluronic acid-based nanoparticles induced higher efficacy of doxorubicin for breast carcinoma therapy

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Biodegradable micelles with sheddable poly(ethylene glycol) shells for triggered intracellular release of doxorubicin

Cited by 422 publications

References 43 publications

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

pH-Sensitive Micelles for Targeted Drug Delivery Prepared Using a Novel Membrane Contactor Method

Self-assembled mPEG&ndash;PCL-g&ndash;PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro

GE11 peptide modified and reduction-responsive hyaluronic acid-based nanoparticles induced higher efficacy of doxorubicin for breast carcinoma therapy

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Self-assembled mPEG–PCL-g–PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro