Biodegradable Polymer Based Particulate Carrier(s) for the Delivery of Proteins and Peptides

Mishra, Neeraj; Goyal, Amit K.; Khatri, Kapil; Vaidya, Bhuvaneshwar; Paliwal, Rishi; Rai, Shivani; Mehta, Abhinav; Tiwari, Shivani; Vyas, Sameer; Vyas, Suresh P.

doi:10.2174/187152308786847816

Cited by 42 publications

(21 citation statements)

References 84 publications

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“…Accordingly, chemical bonds of FHA and PCL are identified in all spectra of PCL/FHA mats. (PO 3 ) 4− group are identified around 400 to 1100 cm −1 . In addition, all PCL characteristic groups were identified, namely ─(CH 2 ) 4 ─ group, C═O, and C─O bonds observed around 3000 to 2850 cm −1 and 1250 to 1450 cm −1 , at 1750 cm −1 , and around 1150 to 1250 cm −1 …”

Section: Resultsmentioning

confidence: 87%

The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering

Johari

Fathi

Fereshteh

et al. 2019

Polymers for Advanced Techs

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Biodegradable cell-incorporated scaffolds can guide the regeneration process of bone defects such as physiological resorption, tooth loss, and trauma which medically, socially, and economically hurt patients. Here, 0, 5, 10, and 15 wt% fluoridated hydroxyapatite (FHA) nanoparticles containing 25 wt% F − and 75 wt% OH − were incorporated into poly (ε-caprolactone) (PCL) matrix to produce PCL/FHA nanocomposite scaffolds using electrospinning method. Then, scanning electron microscopy (SEM), X-ray diffraction (XRD) pattern, and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the morphology, phase structure, and functional groups of prepared electrospun scaffolds, respectively. Furthermore, the tensile strength and elastic modulus of electrospun scaffolds were investigated using the tensile test. Moreover, the biodegradation behavior of electrospun PCL/FHA scaffolds was studied by the evaluation of weight loss of mats and the alternation of pH in phosphate buffer saline (PBS) up to 30 days of incubation.Then, the biocompatibility of prepared mats was investigated by culturing MG-63 osteoblast cell line and performing MTT assay. In addition, the adhesion of osteoblast cells on prepared electrospun scaffolds was studied using their SEM images. Results revealed that the fiber diameter of prepared electrospun PCL/FHA scaffolds alters between 700 and 900 nm. The mechanical assay illustrated the mat with 10 wt% FHA nanoparticles revealed the highest tensile strength and elastic modulus. The weight loss alternation of mats determined around 1% to 8% after 30 days of incubation. The biocompatibility and cell adhesion of mats improved by increasing the amounts of FHA nanoparticles. K E Y W O R D S biocompatibility, electrospinning, nano-fluoridated hydroxyapatite, poly(ɛ-caprolactone), tensile strength 1 | INTRODUCTION Fibrous structure scaffolds exhibits the similar size to the extracellular matrix structure, protein absorption, and cell attachment in tissue engineering applications. 1 As an effective and low-cost fabricating method, electrospinning has been applied to produce continuous fibers from the nanoscale to the microscale. 2 Tissue engineering scaffolds should essentially possess several properties such as desirable biocompatibility, simple fabrication, controllable degradation rate, and sufficient mechanical properties. Accordingly, diverse synthetic polymers have been electrospun and modified as well as natural polymersto perform in different tissues. 3 Among these, poly(ε-caprolactone) (PCL) as a synthetic and biodegradable polymer possesses the biocompatibility and rheological and viscoelastic properties, prepared as a fibrous structure via the electrospinning. 4-6 Hence, PCL fibers can be selected as an appropriate candidate for tissue engineering scaffolds, regenerating skin, cartilage, bone, and cardiac constructs. 7 PCL is a polyester with a semicrystalline structure and amorphous domains,

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Section: Resultsmentioning

confidence: 87%

The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering

Johari

Fathi

Fereshteh

et al. 2019

Polymers for Advanced Techs

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“…In addition to topical skin delivery, chitosan‐based systems have also been reported in oral and transmucosal delivery applications . Chitosan appears to boost immune responses by enhancing the uptake of antigen across nasal mucosa and as a result, it is a good candidate for effective mucosal vaccine delivery systems .…”

Section: Polysaccharidesmentioning

confidence: 99%

Biodegradable polymers as encapsulation materials for cosmetics and personal care markets

Ammala

2012

Intern J of Cosmetic Sci

160

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SynopsisThe topical and transdermal delivery of active cosmetic ingredients requires safe and non-toxic means of reaching the target sites without causing any irritation. Preservation of the active ingredients is also essential during formulation, storage and application of the final product. As many biologically active substances are not stable and sensitive to temperature, pH, light and oxidation, they require encapsulation to protect against unwanted degradation and also to target specific and controlled release of the active substance. The use of biodegradable polymers as encapsulation materials offers several advantages over other carrier materials. Encapsulation of active ingredients using biodegradable polymeric carriers can facilitate increased efficacy and bioavailability and they are also removed from the body via normal metabolic pathways. This article reviews current research on biodegradable polymers as carrier or encapsulation materials for cosmetic and personal care applications. Some of the challenges and limitations are also discussed. Examples of biodegradable polymers reviewed include polysaccharides, poly a-esters, polyalkylcyanoacrylates and polyamidoamine dendrimers. Ré suméL'administration topique et transdermique de principes actifs cosmétiques exige des moyens sû rs et non toxiques pour atteindre les sites cibles sans causer d' irritation. La préservation des ingrédients actifs est également essentielle au cours de la formulation, du stockage et de l'application du produit final. Étant donné que beaucoup de substances biologiquement actives ne sont pas stables et sensibles à la température, le pH, la lumière et l'oxydation, elles ont besoin d'encapsulation pour les protéger contre la dégradation indésirable et également de cibler la libération spécifique et contrôlée de la substance active. L'utilisation de polymères biodégrad-ables comme matériaux d'encapsulationoffre plusieurs avantages par rapport aux autres matériaux porteurs. L'encapsulation des ingrédients actifs à l'aide des supports polymères biodégradables peut faciliter l'augmentation de l'efficacité et de la biodisponibilité; ceux-cisont également éliminés de l'organisme par l'intermédiaire des voies métaboliques normales. Cet article passe en revue les recherches actuelles sur les polymères biodégradables comme matériaux de support ou d'encapsulation pour les cosmétiques et les applications de soins personnels. Les défis et les limites sont également discutés. Des exemples de polymères biodégradables passés en revue comprennent des polysaccharides, des poly-a-esters, des polyalkylcyanoacrylates et les polyamidoaminedendrimères.

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“…The development of a successful delivery system for protein therapeutic requires a thorough understanding of not only the physical and chemical behavior of the individual protein and the application of sophisticated analytical methods, but also the physiological characteristics of protein molecules, including their molecular size, biological halflife, immunogenicity, conformational stability, dose requirement, site and rate of administration, pharmacokinetics and pharmacodynamic behavior [87]. The therapeutic activity of proteins is highly dependent on their conformational structure.…”

Section: Physical and Chemical Compatibility Between Chitosan And Promentioning

confidence: 99%

Chitosan Formulations as Carriers for Therapeutic Proteins

Andrade

Antunes²,

Nascimento³

et al. 2011

CDDT

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Protein drugs represent a significant part of the new pharmaceuticals coming on the market every year and are now widely spread in therapy to treat or relief symptomatology related to many metabolic and oncologic diseases. The delivery of therapeutic proteins is still a major drawback against their maximum pharmacodynamic due to their physicochemical properties, poor stability, permeability and biodistribution. Despite the fact that the parenteral route remains the primary route of protein administration, research continues on non-parenteral delivery routes. However, the high molecular weight of proteins, combined with their hydrophilic and charged nature, renders transport through membranes very difficult. In this regard, the biopolymer chitosan exhibits several favorable biological properties, such as biocompatibility, biodegradability, low-toxicity and mucoadhesiveness, which made it a promising candidate for the formulation of protein drugs. The success of a protein formulation depends not only on the stability of the delivery system but also on their ability to maintain the native structure and activity of the protein during preparation and the delivery, as well as during longterm storage of the formulation. Chitosan-based delivery systems have been proposed as valid approaches to provide such protective conditions. The development of novel protein delivery systems based on chitosan is a rising subject irrespective of the intended route of administration. In this review, the different approaches recently exploited to formulate and deliver therapeutic proteins are underlined.

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Biodegradable Polymer Based Particulate Carrier(s) for the Delivery of Proteins and Peptides

Cited by 42 publications

References 84 publications

The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering

The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering

Biodegradable polymers as encapsulation materials for cosmetics and personal care markets

Chitosan Formulations as Carriers for Therapeutic Proteins

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