Biodegradation and bioresorption of poly(ɛ-caprolactone) nanocomposite scaffolds

Mkhabela, Vuyiswa J.; Ray, Suprakas Sinha

doi:10.1016/j.ijbiomac.2015.04.056

Cited by 30 publications

(9 citation statements)

References 31 publications

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“…Bio‐degradation is one of the most important and vital features of scaffolds to be utilized in biomedical purposes 58‐62 . In the tissue engineering context, the control and engineering of the degradation extent of fabricated scaffolds can be considered as an important issue 13,62,63 .…”

Section: Resultsmentioning

confidence: 99%

Tuning polylactic acid scaffolds for tissue engineering purposes by incorporating graphene oxide‐chitosan nano‐hybrids

Majidi

Babaei

Kazemi‐Pasarvi

et al. 2020

Polymers for Advanced Techs

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The main goal of this study was to take an effective approach to promote polylactic acid (PLA) bio‐applications. In this regard, graphene oxide (GO) and spherical structure of GO‐chitosan nano‐hybrids (GO‐CS) were successfully synthesized and characterized using transmission electron microscopy, atomic force microscopy, Fourier‐transform infrared, UV–Vis, and elemental analyses. Thereafter, PLA scaffolds containing 1 wt% and 2 wt% of synthesized nanomaterials were fabricated through the solvent casting/particulate leaching procedure. Then, the biodegradability, cell adhesion, and bio‐safety properties of the prepared scaffolds were investigated. The results of bio‐degradation analyses demonstrated that the used nanomaterials prevented PLA from rapid and excessive degradation. The cell viability and contact angle results revealed that the surface hydrophilicity of PLA scaffolds was noticeably enhanced using both GO and GO‐CS nanoparticles. Although scaffolds containing GO represented a more hydrophilic surface, their cell viability reduced as a consequence of GO cytotoxicity. It was also revealed that incorporation of GO‐CS nano‐hybrids into PLA scaffold tuned the cell viability through facilitating cell adhesion, growth, and proliferation processes.

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Section: Resultsmentioning

confidence: 99%

Tuning polylactic acid scaffolds for tissue engineering purposes by incorporating graphene oxide‐chitosan nano‐hybrids

Majidi

Babaei

Kazemi‐Pasarvi

et al. 2020

Polymers for Advanced Techs

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“…At 2 weeks the collagen tubes were still present, but electrospun fibres were not visible by light microscopy in cross‐sections. This was at first surprising because degradation experiments in buffer indicated that PCL is relatively stable, resulting in degradation rates from below 1% (Kim et al , ) to 20% (Mkhabela and Ray, ) within 2 weeks. Biological degradation, however, is much faster.…”

Section: Discussionmentioning

confidence: 99%

Cell-free artificial implants of electrospun fibres in a three-dimensional gelatin matrix support sciatic nerve regeneration in vivo

Kriebel

Hodde

Kuenzel

et al. 2017

J Tissue Eng Regen Med

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Surgical repair of larger peripheral nerve lesions requires the use of autologous nerve grafts. At present, clinical alternatives to avoid nerve transplantation consist of empty tubes, which are only suitable for the repair over short distances and have limited success. We developed a cell-free, three-dimensional scaffold for axonal guidance in long-distance nerve repair. Sub-micron scale fibres of biodegradable poly-ε-caprolactone (PCL) and collagen/PCL (c/PCL) blends were incorporated in a gelatin matrix and inserted in collagen tubes. The conduits were tested by replacing 15-mm-long segments of rat sciatic nerves in vivo. Biocompatibility of the implants and nerve regeneration were assessed histologically, with electromyography and with behavioural tests for motor functions. Functional repair was achieved in all animals with autologous transplants, in 12 of 13 rats that received artificial implants with an internal structure and in half of the animals with empty nerve conduits. In rats with implants containing c/PCL fibres, the extent of recovery (compound muscle action potentials, motor functions of the hind limbs) was superior to animals that had received empty implants, but not as good as with autologous nerve transplantation. Schwann cell migration and axonal regeneration were observed in all artificial implants, and muscular atrophy was reduced in comparison with animals that had received no implants. The present design represents a significant step towards cell-free, artificial nerve bridges that can replace autologous nerve transplants in the clinic. Copyright © 2017 John Wiley & Sons, Ltd.

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“…To ensure the possibility of chemical modification and high multifunctionality, the preference in the development of scaffolds is given to artificial materials, one of which is polycaprolactone (PCL) [3]. This polymer is capable of biodegradation in vivo with formation of the products without toxic effect on surrounding tissues and the whole body [4].…”

Section: Introductionmentioning

confidence: 99%

Evaluating biocompatibility of vaterite-mineralized polycaprolactone matrices in subcutaneous implantation tests on white rats

et al. 2020

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Objective: to estimate biocompatibility of matrices manufactured from polycaprolactone (PCL) and mineralized by vaterite (CaCO3) via studying local and systemic manifestations of inflammatory reaction in subcutaneous implantation tests on white rats. Material and Methods. The experiment was conducted on 40 rats divided into four groups of equal sizes: control, comparison (rats with imitation of implantation), negative control (rats with implanted non-biocompatible matrices) and experimental group, comprised of animals with implanted PCL/CaCO3-matrices. Local inflammatory manifestations were analyzed by morphological investigation of implantation area tissues. Systemic inflammatory manifestations were estimated via TNF-α and interleukin-1β (IL-1) concentrations in blood serum by ELISA. Results. The changes in cellular population content demonstrated that, on day 21 after the implantation, the PCL/CaCO3-matrice was evenly colonized by fibroblast cells and afterwards vascularized. Such matrices did not cause intense inflammatory reaction observed in negative control animals. It was accompanied by systemic manifestations, such as statistically significant increase in TNF-α and IL-1 concentrations. Conclusion. Our data confirmed the biocompatibility of PLC/CaCO3-scaffolds, thus experimentally substantiating the potential for their use in tissue engineering.

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Biodegradation and bioresorption of poly(ɛ-caprolactone) nanocomposite scaffolds

Cited by 30 publications

References 31 publications

Tuning polylactic acid scaffolds for tissue engineering purposes by incorporating graphene oxide‐chitosan nano‐hybrids

Tuning polylactic acid scaffolds for tissue engineering purposes by incorporating graphene oxide‐chitosan nano‐hybrids

Cell-free artificial implants of electrospun fibres in a three-dimensional gelatin matrix support sciatic nerve regeneration in vivo

Evaluating biocompatibility of vaterite-mineralized polycaprolactone matrices in subcutaneous implantation tests on white rats

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