Biodegradation Kinetics of Tetrahydrofuran, Benzene, Toluene, and Ethylbenzene as Multi-substrate by Pseudomonas oleovorans DT4

Abstract

The biodegradation kinetics of tetrahydrofuran, benzene (B), toluene (T), and ethylbenzene (E) were systematically investigated individually and as mixtures by a series of aerobic batch degradation experiments initiated by Pseudomonas oleovorans DT4. The Andrews model parameters, e.g., maximum specific growth rates (μmax), half saturation, and substrate inhibition constant, were obtained from single-substrate experiments. The interaction parameters in the sum kinetics model (SKIP) were obtained from the dual substrates. The μmax value of 1.01 for tetrahydrofuran indicated that cell growth using tetrahydrofuran as carbon source was faster than the growth on B (μmax, B = 0.39) or T (μmax, T = 0.39). The interactions in the dual-substrate experiments, including genhancement, inhibition, and co-metabolism, in the mixtures of tetrahydrofuran with B or T or E were identified. The degradation of the four compounds existing simultaneously could be predicted by the combination of SKIP and co-metabolism models. This study is the first to quantify the interactions between tetrahydrofuran and BTE.

Biodegradation kinetics and interactions of styrene and ethylbenzene as single and dual substrates for a mixed bacterial culture

“…The inhibition coefficients K I for ethylbenzene was estimated to be 62.62 mg/L. Compared with previous studies, the relatively high value of K I for ethylbenzene indicated that the mix culture was less sensitive to substrate inhibition [ 38 ].…”

Isolation of Pseudomonas oleovorans Carrying Multidrug Resistance Proteins MdtA and MdtB from Wastewater

“…Previous studies showed that Pseudomonas oleovorans , which was isolated from estuarine sediment, can degrade polycyclic aromatic hydrocarbons [ 17 , 18 ]. A series of aerobic batch degradation experiments showed that Pseudomonas oleovorans can biodegrade tetrahydrofuran, benzene, toluene, and ethylbenzene [ 19 , 20 , 21 ]. However, the physicochemical properties and drug resistance of Pseudomonas oleovorans are still unclear.…”

Stability Analysis of a Chemostat Model for Phenol and Sodium Salicylate Mixture Biodegradation

“…The following models are widely used in the analysis and control of the wastewater treatment processes involving mixtures of pollutant components: sum kinetics with interaction parameters (SKIP), self-inhibition EC-SKIP (SIEC-SKIP), elimination capacity-sum kinetics with interaction parameter (EC-SKIP), etc. [22][23][24][25]. The SKIP models describe well the biodegradation by different microorganisms of various mixtures of interacting phenolic pollutants in wastewater: phenol and p-cresol or phenol and resorcinol by Gliomastix indicus MTCC 3869 [12]; phenol and SA by Pseudomonas putida [14][15][16]; 4-bromophenol and 4-chlorophenol by Arthrobacter chlorophenolicus A6 [26].…”