Biodegradation mechanism of chloramphenicol by Aeromonas media SZW3 and genome analysis

Tan, Zewen; Yang, Xiaoyan; Chen, Lian; Liu, Yiling; Xu, Huijuan; Li, Yongtao; Gong, Beini

doi:10.1016/j.biortech.2021.126280

Cited by 23 publications

(1 citation statement)

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“…A. media was first isolated from river freshwater and is often isolated from sewage water, activated sludge, drinking water, earthworm gut content, chicken, animals, and humans [ 32 , 33 ]. It is an opportunistic pathogen that causes serious diseases in aquatic organisms (fish and other cold-blooded species) and humans, particularly as foodborne pathogens [ 4 , 32 , 34 ]. Strain QST31 was isolated from diseased tadpoles and had an LC 50 of 2.69 × 10 7 CFU/mL from bathing infection, indicating that this strain was highly virulent to tadpoles.…”

Section: Discussionmentioning

confidence: 99%

A Novel Transposon Tn7709 Harbors Multidrug Resistance Genes in a Pathogenic Aeromonas media Strain QST31

Shang,

Li,

Zhang

et al. 2024

Microorganisms

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Pathogenic Aeromonas spp. are the etiological agents of Motile Aeromonas Septicemia (MAS). This study aimed to identify the pathogen of diseased tadpoles (Quasipaa spinosa) and the antibiotic-resistance characteristics of this bacterium. A Gram-negative bacterium, named strain QST31, was isolated from the ascites of diseased tadpoles and was identified as Aeromonas media based on physiological and biochemical tests, as well as molecular identification. Artificial infection experiments showed that strain QST31 was highly virulent to tadpoles, with an LC50 of 2.56 × 107 CFU/mL. The antimicrobial susceptibility of strain QST31 was evaluated using the disk diffusion method, and the results indicated that strain QST31 was resistant to 28 antibacterial agents. In addition, the whole genome of strain QST31 was sequenced, and the presence of antimicrobial resistance genes, integron, and transposon was investigated. Genes involved in adherence, hemolysis, type II secretion system (T2SS), T6SS, iron uptake system, and quorum sensing were identified in the genome of strain QST31. More than 12 antimicrobial resistance genes were predicted in the genome of strain QST31. Interestingly, a novel Tn7709 transposon harboring sul1, aadA16, catB3, blaOXA-21, aac(6′)-IIa, and tet(A) genes was identified. In conclusion, this is the first report on the isolation and identification of pathogenic A. media with multidrug resistance genes from diseased tadpoles. The results revealed that preventing and controlling aquatic animal diseases caused by multidrug resistance A. media will be a huge challenge in the future.

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