Biodegradation of 2,4-dichlorophenol in a fluidized bed reactor with immobilized Phanerochaete chrysosporium

Abstract

The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP) from aqueous solution was investigated. The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP degradation was examined. Results showed that Lip and Mnp were not essential to 2,4-DCP degradation while their presence enhanced the degradation process and reaction rate. In sequential batch experiment, the bioactivity of immobilized cells was recovered and improved during the culture and the maximum degradation rate constant of 13.95 mg (Ld)⁻¹ could be reached. In continuous bioreactor test, the kinetic behavior of the Phanerochaete chrysosporium immobilized on loofa sponge was found to follow the Monod equation. The maximum reaction rate was 7.002 mg (Lh)⁻¹, and the saturation constant was 26.045 mg L⁻¹.

Optimization of White-Rot Fungi Mycelial Culture Components for Bioremediation of Pharmaceutical-Derived Pollutants

“…Li et al showed that a certain pre-exponential phase could be observed, with the duration time estimated to last 72 h [67], whereas Zhang et al reported a 24-hour-long exponential phase [69]. The duration of exponential growth and the inhibition growth phase (4 days, approximately) was confirmed mainly by previous reports, where it was equal to 5-6 days [66,67].…”

“…In contrast, the adaptation and acceleration phases were not very well pronounced, according to results for optimum-growing medium [66,67], cotton stalks [68], or corn-cob extract [66]. Li et al showed that a certain pre-exponential phase could be observed, with the duration time estimated to last 72 h [67], whereas Zhang et al reported a 24-hour-long exponential phase [69]. The duration of exponential growth and the inhibition growth phase (4 days, approximately) was confirmed mainly by previous reports, where it was equal to 5-6 days [66,67].…”

“…This species grew in the exponential phase practically from the beginning of cultivation. In contrast, the adaptation and acceleration phases were not very well pronounced, according to results for optimum-growing medium [66,67], cotton stalks [68], or corn-cob extract [66]. Li et al showed that a certain pre-exponential phase could be observed, with the duration time estimated to last 72 h [67], whereas Zhang et al reported a 24-hour-long exponential phase [69].…”

Fermentation: A Potential Strategy for Microbial Metabolite Production

“…It is a plug flow reactor that belongs to the group where back mixing is not present; but in this reactor small amount of back mixing occurs that affects the attributes of the fermentation (Figure 8) [43]. Fluidized-bed bioreactor (Source: [41]).…”

A review on sustainable iron oxide nanoparticles: syntheses and applications in organic catalysis and environmental remediation

“…[490][491][492] Another crucial arena is the immobilization of biomaterials onto a sturdier and porous support that has been significantly investigated for varied applications; examples can be found for fungi and microalgae and exploitation for hydrogen production. [493][494][495][496][497] Indeed, immobilized cells have garnered a huge attention since the 1970s, because of their clear merits over dispersed cells. 498 First, the immobilization of native biomaterial improves the physical properties and proposes advanced degree of action.…”