Biodegradation of 3-chlorobenzoate and 3-hydroxybenzoate by polyurethane foam immobilized cells of Bacillus sp. OS13

Mulla, Sikandar I.; Manjunatha, Bangeppagari; Mahadevan, G.; Eqani, Syed Ali Musstjab Akber Shah; Sajjan, Dayanand B.; Tallur, Preeti N.; Megadi, Veena B.

doi:10.1016/j.jece.2016.02.027

Cited by 13 publications

(6 citation statements)

References 42 publications

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“…colonization and CP removal; therefore, PF was selected for further removal studies using a CSTR. The use of PF as a support for cell immobilization has proven effective in the removal of different contaminants such as petroleum oil, chloroaromatic compounds and OP pesticides (Yadav et al 2014;Mulla et al 2016;Alessandrello et al 2017).…”

Section: Removal Of Pesticides By Immobilized Streptomyces Spp Mixedmentioning

confidence: 99%

Performance of a continuous stirred tank bioreactor employing an immobilized actinobacteria mixed culture for the removal of organophosphorus pesticides

et al. 2020

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In this study, we evaluated polyurethane foam (PF), volcanic rock (VR), and a modified plastic cap (MPC) as supports for the immobilization of organophosphorus (OP) pesticide-degrading actinobacterial strains. The colonization and activity of four streptomycetes were favoured by PF, which was selected as the carrier to use in a continuous stirred tank bioreactor (CSTR) that can be operated at increasing inflows of a pesticide mixture that contains the insecticides chlorpyrifos (CP) and diazinon (DZ). Our results demonstrate that the CSTR can be operated at flow rates of 10 and 40 mL h −1 with greater than 85% removal of the pesticides in the short term. A significant decrease in the efficiency of CP removal was observed at the highest inflows into the reactor. The CP and DZ loading rates in the bioreactor ranged from 0.44 to 1.68 mg L −1 h −1 and from 0.50 to 2.17 mg L −1 h −1 , respectively. Although the treated wastewater exhibited moderate toxicity for Raphanus sativus, a bioreactor inoculated with a mixed culture formed by Streptomyces spp. strains AC5, AC9, GA11 and ISP13 may provide an effective biotechnological strategy for the reduction of OP pesticide residues produced during agronomic and manufacturing practices and therefore prevent environmental pesticidal pollution.

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Section: Removal Of Pesticides By Immobilized Streptomyces Spp Mixedmentioning

confidence: 99%

Performance of a continuous stirred tank bioreactor employing an immobilized actinobacteria mixed culture for the removal of organophosphorus pesticides

et al. 2020

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“…L6 and Bacillus sp. OS13) have been predicted to metabolize 3-CBA via PC and/or GS rather than via CC [ 30 , 31 , 32 , 34 ]. In these pathways, the intermediate compounds include 4-hydroxybenzoate (4-HB), 3-hydroxybenzoate (3-HB), protocatechuate (PC) and gentisate (GS).…”

Section: Resultsmentioning

confidence: 99%

“…Alternatively, based on analyses of the intermediate compounds of 3-CBA degradation, it has been predicted that 3-CBA can also be degraded via 4-hydroxybenzoate (4-HB) or 3-hydroxybenzoate (3-HB) [ 30 , 31 , 32 , 33 , 34 ]. These compounds are converted to protocatechuate (PC) or gentisate (GS) and, therefore, these pathways are known as the PC and GS pathways, respectively [ 30 , 31 , 32 , 33 , 34 ]. The initial enzyme responsible for the conversion of 3-CBA to 4-HB or 3-HB has not yet been identified [ 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…These compounds are converted to protocatechuate (PC) or gentisate (GS) and, therefore, these pathways are known as the PC and GS pathways, respectively [ 30 , 31 , 32 , 33 , 34 ]. The initial enzyme responsible for the conversion of 3-CBA to 4-HB or 3-HB has not yet been identified [ 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…L6 GS and PC [ 32 ] Bacillus sp. OS13 PC [ 30 ] Pseudomonas GS [ 31 ] Abbreviations: 3-CBA, 3-chlorobenzoate; CC, chlorocatechol; GS, gentisate; PC, protocatechuate. …”

Section: Introductionmentioning

confidence: 99%

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Isolation and Genomic Analysis of 3-Chlorobenzoate-Degrading Bacteria from Soil

et al. 2023

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The compound 3-chlorobenzoate (3-CBA) is a hazardous industrial waste product that can harm human health and the environment. This study investigates the physiological and genetic potential for 3-chlorobenzoate (3-CBA) degradation. Six 3-CBA Gram-negative degraders with different degradation properties belonging to the genera Caballeronia, Paraburkholderia and Cupriavidus were isolated from the soil. The representative strains Caballeronia 19CS4-2 and Paraburkholderia 19CS9-1 showed higher maximum specific growth rates (µmax, h−1) than Cupriavidus 19C6 and degraded 5 mM 3-CBA within 20–28 h. Two degradation products, chloro-cis,cis-muconate and maleylacetate, were detected in all isolates using high-performance liquid chromatography and mass spectrometry. Genomic analyses revealed the presence of cbe and tfd gene clusters in strains 19CS4-2 and 19CS9-1, indicating that they probably metabolized the 3-CBA via the chlorocatechol ortho-cleavage pathway. Strain 19C6 possessed cbe genes, but not tfd genes, suggesting it might have a different chlorocatechol degradation pathway. Putative genes for the metabolism of 3-hydroxybenzoate via gentisate were found only in 19C6, which utilized the compound as a sole carbon source. 19C6 exhibited distinct characteristics from strains 19CS4-2 and 19CS9-1. The results confirm that bacteria can degrade 3-CBA and improve our understanding of how they contribute to environmental 3-CBA biodegradation.

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