Biodegradation of anthracene and phenanthrene by bacteria isolated from oil-contaminated soil of Bangladesh

Chowdhury, Rocky; Dhar, Kartik; Ahamed, Forkan; Alam, Shawkat; Uddin, Mohammad Helal

doi:10.1080/02757540.2017.1382481

Cited by 11 publications

(6 citation statements)

References 39 publications

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“…The ability of Stenotrophomonas sp. to degrade PAHs and other hydrocarbons has been demonstrated (Tiwari et al, 2016; Nowak and Mrozik, 2018) and in particular, S. maltophilia strains (Chowdhury et al, 2017; Kumari et al, 2018). The strain S. maltophilia AJH1 was able to degrade low and high molecular weight PAHs (up to 95 and 80%, respectively) in acidophilic medium at pH 2 (Arulazhagan et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

et al. 2019

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A PAHs-contaminated industrial soil was analyzed using PCR amplification of the gene 16S ribosomal RNA for the detection and identification of different isolated bacterial strains potentially capable of degrading PAHs. Novel degrader strains were isolated and identified as Achromobacter xylosoxidans 2BC8 and Stenotrophomonas maltophilia JR62, which were able to degrade PYR in solution, achieving a mineralization rate of about 1% day–1. A. xylosoxidans was also able to mineralize PYR in slurry systems using three selected soils, and the total extent of mineralization (once a plateau was reached) increased 4.5, 21, and 57.5% for soils LT, TM and CR, respectively, regarding the mineralization observed in the absence of the bacterial degrader. Soil TM contaminated with PYR was aged for 80 days and total extent of mineralization was reduced (from 46 to 35% after 180 days), and the acclimation period increased (from 49 to 79 days). Hydroxypropyl-ß-cyclodextrin (HPBCD) was used as a bioavailability enhancer of PYR in this aged soil, provoking a significant decrease in the acclimation period (from 79 to 54 days) due to an increase in PYR bioavailable fraction just from the beginning of the assay. However, a similar global extension of mineralization was obtained. A. xylosoxidans was then added together with HPBCD to this aged TM soil contaminated with PYR, and the total extent of mineralization decreased to 25% after 180 days, possibly due to the competitive effect of endogenous microbiota and the higher concentration of PYR in the soil solution provoked by the addition of HPBCD, which could have a toxic effect on the A. xylosoxidans strain.

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Section: Resultsmentioning

confidence: 99%

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

et al. 2019

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“…The ability of the isolates to utilize anthracene substrate can be measured by observing the disappearance of the blue color of the 2,6-DCPIP with time ( Karale et al, 2015 ). The 2,6-DCPIP assay was carried out according to the method of Chowdhury et al (2017) with modifications. The assay was carried out to evaluate the capability of single or combined isolates for the biodegradation of anthracene.…”

Section: Methodsmentioning

confidence: 99%

“…The equation Y = 501×−0.0071 was obtained from the curve, y represents the OD of DCPIP of each × indicates the corresponding concentration in mg/ml ( Supplementary Figure 1 ). The percentage of remaining DCPIP color intensity was calculated by comparing the change in color intensity after a given time with the initial value based on Chowdhury et al, 2017 equation:…”

Section: Methodsmentioning

confidence: 99%

High Counts and Anthracene Degradation Ability of Streptococcus mutans and Veillonella parvula Isolated From the Oral Cavity of Cigarette Smokers and Non-smokers

et al. 2021

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The composition and metabolic functions of oral microbiota are affected by many factors including smoking leading to several health problems. Cigarette smoking is associated with changes in oral microbiota composition and function. However, it is not known if the depletion of certain bacterial genera and species is due to specific toxins in cigarette smoke, or indirectly due to competition for colonization with smoking-enriched bacteria. Therefore, the aim of this study was to determine the effect of cigarette smoking on the microbial prevalence and polycyclic aromatic hydrocarbons (PAHs) biodegradation of selected enriched and depleted oral bacteria from oral microbiota of smokers compared to that in non-smokers. Samples of oral rinse from smokers and non-smokers were collected (n = 23, 12 smokers and 11 non-smokers) and screened for oral bacterial strains of Streptococcus mutans, Lactobacillus spp., and Veillonella spp. Comparing counts, S. mutans, V. tobetsuensis, and V. dispar showed higher counts in smokers compared to non-smokers while the Lactobacillus spp. were higher in non-smokers. Lactobacillus fermentum was prevalent in smokers, representing 91.67% of the total Lactobacillus spp. isolates. The biodegradation potential of anthracene; a representative of PAHs of collected isolates, in single and mixed cultures, was assayed with anthracene as the sole source of carbon using 2,6-dichlorophenol indophenol (2,6-DCPIP) as indicator. S. mutans isolates recovered from smokers showed higher degradation of anthracene compared to those recovered from non-smokers. The anaerobic anthracene biodegradation activity of V. parvula isolates from non-smokers was the highest among all isolates of the three recovered genera from the same subject. The anthracene biodegradation potential of Lactobacillus spp. was variable. Combinations of isolated bacteria in co-cultures showed that Lactobacillus spp. interfered with anthracene biodegradation ability along with the viable counts of S. mutans and Veillonella spp. In conclusion, oral dysbiosis due to cigarette smoking was observed not only due to changes in oral bacterial relative abundance but also extended to bacterial functions such as anthracene biodegradation tested in this study. Microbe–microbe interactions changed the anthracene biodegradation potential and growth of the microbial mixture compared to their corresponding single isolates, and these changes differ according to the constituting bacteria.

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“…Genus Pseudomonas is dominant in both contaminated and uncontaminated soil even though the present of Pseudomonas in contaminated clay was not indicated. Many reports indicated the ability of Pseudomonas aeruginosa and Pseudomonas fluorescens in degrading hydrocarbons including diesel oil and other PAHs such as anthracene and phenanthrene [7,8]. Gram-positive Staphylococcus was also one of hydrocarbon degraders that were vastly studied especially for Staphylococcus aureus [9].…”

Section: Bacterial Identificationmentioning

confidence: 99%

Bacterial Population Study in Oil-Contaminated and Uncontaminated Soils

Nordin¹,

Mahassan²,

Chowdhury³

2018

J. CleanWAS

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Soil provides a vital habitat primarily for bacteria to continue the process of biogeochemical cycle. The remarkable metabolic diversity and capacity of the soil bacteria is increasingly being harnessed for the benefit of humankind. One of the beneficial spin-offs from the understanding of the metabolism of soil microbes is the development of bioremediation for contaminated soils. Investigation on bacterial diversity was conducted on several types of samples for both contaminated and uncontaminated soils. The soil texture test determined that the soil samples obtained were sandy, silt and clay, respectively. The microbial population were enumerated using colony counting while bacteria characterisation and identification were performed using Gram staining, morphological and biochemical analysis. This study indicated that microbial population is higher in hydrocarbon-contaminated soils compared to uncontaminated soils. On the other hands, the bacterial diversity is lower in contaminated soils compared to uncontaminated soils. The study also portrays that Gram-positive bacteria, Staphylococcus spp was dominant in untreated soils while Gram negative bacteria, Pseudomonas spp was dominant genus in hydrocarbon contaminated soil.

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Biodegradation of anthracene and phenanthrene by bacteria isolated from oil-contaminated soil of Bangladesh

Cited by 11 publications

References 39 publications

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

High Counts and Anthracene Degradation Ability of Streptococcus mutans and Veillonella parvula Isolated From the Oral Cavity of Cigarette Smokers and Non-smokers

Bacterial Population Study in Oil-Contaminated and Uncontaminated Soils

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