Biodegradation of bilge waste from Patagonia with an indigenous microbial community

Nievas, Marina L.; Commendatore, Marta; Olivera, Nelda Lila; Esteves, JoséL.; Bucalá, Verónica

doi:10.1016/j.biortech.2005.10.042

Cited by 45 publications

(38 citation statements)

References 38 publications

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“…Aliphatics was found to be 89 % and aromatics was found to be 11%. The obtained results are in agreement with the analysis of Nievas et al (2006Nievas et al ( , 2008 which higher concentration of aliphatics than aromatics in bilge oil waste. According to the observed kinetics of Nievas et al (2008), three main types of hydrocarbons i.e.…”

Section: Resultssupporting

confidence: 90%

“…Nievas et al (2006) quantified total hydrocarbons in Bilge waste oily phase (BWOP) by gas chromatographic analysis. The total hydrocarbons present were 542 g/kg BWOP which includes resolved total hydrocarbons as well as Unresolved complex mixture (UCM).…”

Section: Resultsmentioning

confidence: 99%

“…Illegal discharge of bilge oil into the sea accounts for about 10 % of the total oils that enter the sea and oil pollution is a serious threat to the ecosystem. Bilge wastewater generally includes lubricating oil, cleaning diesel oil, oily sludge, spills from engine room, water leaks from internal pipes and sea water filtrations (Nievas et al, 2006). Hydrocarbons, the main constituent of bilge oil waters are toxic in nature and they are of concern due to their long persistence in the environment.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of hydrocarbonoclastic bacteria from bilge oil contaminated water

Sivaraman

Ganguly

Nikolausz

et al. 2011

Int. J. Environ. Sci. Technol.

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Two bacterial strains, i.e. Pseudomonas mendocina and Ochrobactrum sp. were isolated from bilge oil contaminated water of Mormugao harbour, Goa, India and grown in a culture medium with hexadecane as the sole carbon source. Pseudomonas mendocina was used in further studies as it was the dominant strain. This strain effectively degraded tetradecane, hexadecane and octadecane leaving a residual concentration of about 73 %, 54 % and 40 % respectively in 120 h. Sequence analysis of the dominant bands from the denaturing gradient gel electrophoresis profiles revealed the differences between the genera of bilge oil contaminated sea water and its enrichment culture on hexadecane indicating a shift in community structure based on the type of substrate available. Pseudomonas mendocina amplified for the following catabolic genes namely C23O, nid and ndo. Based on the catabolic gene study the potential of the bacterial strain isolated, i.e. Pseudomonas mendocina seems to be interesting as it will be able to degrade polyaromatic hydrocarbons as well. Physicochemical properties of Pseudomonas mendocina indicates production of exopolysaccharides based on the value of its isoelectric point.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation of hydrocarbonoclastic bacteria from bilge oil contaminated water

Sivaraman

Ganguly

Nikolausz

et al. 2011

Int. J. Environ. Sci. Technol.

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“…The results clearly indicated that no significant difference was observed. Marquez-Rocha et al [57], Nievas et al [58] and Thavasi et al [59] have correspondingly reported up to 70, 68 and 58 percent petroleum hydrocarbon removal from contaminated environment. A full factorial experimental design performed by Pala et al [33] and Mohajeri et al [37] to respectively assess the effects of three and four variables on the bioremediation of petroleum-contaminated soil showed that the hydrocarbon removal rate was around 80 percent.…”

Section: Optimization and Validationmentioning

confidence: 98%

Factorial Designs Application to Study Enhanced Bioremediation of Soil Artificially Contaminated with Weathered Bonny Light Crude Oil through Biostimulation and Bioaugmentation Strategy

Agarry¹,

Ogunleye²

2012

JEP

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The objective of this study was designed to evaluate the effects of biostimulation and bioaugmentation amendment agents (NPK fertilizer, Tween 80 and mixed culture) on the bioremediation of tropical soil samples artificially contaminated with Weathered Bonny Light Crude Oil (WBLCO). Response Surface Methodology (RSM) with Box Behnken Design (BBD) was used with three levels and three factors of NPK fertilizer (2 -6 g), Tween 80 (5 -15 mg/l) and mixed culture (0.5 -1.5 g/l) as independent variables and WBLCO removal as dependent variable (response) in a six weeks remediation period. The results showed that the rate of WBLCO removal generally increased with increase in the amount of NPK fertilizer, Tween 80 and mixed culture (biomass), respectively. A statistically significant (P < 0.0001) second-order quadratic regression model for WBLCO removal (using design-expert statistical program (v. 6.0.8)) with a coefficient of determination, R (=0.9996) was obtained. Numerical optimization technique based on desirability function was carried out to optimize the bioremediation process. The optimum values for biostimulation and bioaugmentation amendment agents to achieve a predicted maximum WBLCO removal of 84.88 percent were found to be: NPK fertilizer, 4.25 g; Tween 80, 10.22 mg/l and mixed culture, 0.46 g/l. At this optimum point, the observed WBLCO removal was found to be 83.79 percent. The statistical analyses and the closeness of the experimental results and model predictions show the reliability of the regression model and thus, biostimulation and bioaugmentation of indigenous microbial density and activity can reduce remediation period of petroleum hydrocarbon contaminated environment and subsequently the cost of remediation.

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“…PAHs, petroleum-and combustion-derived pollutants released from natural (forest fires) and anthropogenic sources (traffic, industrial processes, domestic heating and oil spills), were extracted using this technique from matrices such as water [5,6], seawater [7], wastewater [8], landfill leachates [9], soils [10,11], sediments [12,13], air [14,15], bitumen fumes [16], gasoline soot [17], bilge waste [18], vegetable oils [19], seaweed [20], fish [21] and human samples like blood serum [22] or urine [23]. Headspace (HS) SPME was preferred to fiber immersion when dealing with "dirtier" (particularly solid) materials and gas chromatography with mass spectrometry detection (GC/MS) was predominant in the subsequent quantification step, although liquid chromatography (HPLC) was also used [4].…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted headspace solid-phase microextraction to quantify polycyclic aromatic hydrocarbons in pine trees

2012

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A methodology for the extraction and quantification of 16 polycyclic aromatic hydrocarbons (PAHs) based on microwave-assisted extraction coupled with headspace solid-phase microextraction followed by gas chromatography/mass spectroscopy was validated for needles and bark of two pine species (Pinus pinaster Ait. and Pinus pinea L.). The limits of detection were below 0.92 ng g(-1) (dry weight) for needles and below 0.43 ng g(-1) (dw) for bark. Recovery assays were performed with two sample masses spiked at three levels and the overall mean values were between 70 and 110 % for P. pinaster and 75 and 129 % for P. pinea. In the first species, the increase in sample mass lowered the recoveries slightly for most PAHs, whereas for the second, the recoveries were higher for the needles. Naturally contaminated samples from 4 sites were analysed, with higher levels for urban sites (1,320 and 942 ng g(-1) (dw) vs. 272 and 111 ng g(-1) (dw) for needles and 696 and 488 ng g(-1) (dw) vs. 270 and 103 ng g(-1) (dw) for bark) than for rural ones and also for P. pinaster samples over P. pinea. It is also shown that gas-phase PAHs are predominant in the needles (over 65 % of the total PAHs) and that the incidence for particulate material in bark, reaching 40 % as opposed to a maximum below 20 % for the needles. The method has proved to be fit and improved some of the existing approaches, on the assessment of particulate PAHs and bark levels.

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Biodegradation of bilge waste from Patagonia with an indigenous microbial community

Cited by 45 publications

References 38 publications

Isolation of hydrocarbonoclastic bacteria from bilge oil contaminated water

Isolation of hydrocarbonoclastic bacteria from bilge oil contaminated water

Factorial Designs Application to Study Enhanced Bioremediation of Soil Artificially Contaminated with Weathered Bonny Light Crude Oil through Biostimulation and Bioaugmentation Strategy

Microwave-assisted headspace solid-phase microextraction to quantify polycyclic aromatic hydrocarbons in pine trees

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