Biodegradation of fenitrothion and fenitrooxon by the fungus Trichoderma viride

Baarschers, W. H.; Heitland, Holger S.

doi:10.1021/jf00070a029

Cited by 31 publications

(18 citation statements)

References 6 publications

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“…Studies of the biodegradation of organophosphorus insecticides by fungi include degradation of fenitrothion by Trichoderma viride, Mortierella isabellina and Saprolegnia parasitica (Baarschers and Heitland 1986) and chlorpyrifos, fonofos and terbufos by Phanerochaete chrysosporium (Bumpus et al 1993). However, the present study appears to be the first to report the integration and expression of a versatile bacterial degradation gene in fungi.…”

Section: Discussionmentioning

confidence: 75%

Expression of organophosphate hydrolase in the filamentous fungus Gliocladium virens

Dave

Lauriano

et al. 1994

Appl Microbiol Biotechnol

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The broad-spectrum organophosphate hydrolase (OPH; EC 3.1.8.1) encoded by the organophosphate-degrading gene (opd) from Pseudomonas diminuta MG and Flavobacterium sp. ATCC 27551 possesses capabilities of both P-O bond hydrolysis (e.g. paraoxon) and P-F bond hydrolysis [e.g. sarin and diisopropylfluorophosphate (DFP)]. In the present study a 9.4-kb plasmid, pCL1, was used to transform the saprophytic fungus Gliocladium virens. pCL1 was derived from pJS294 by placing the fungal promoter (prom1) from Cochliobolus heterostrophus upstream and the trpC terminator from Aspergillus nidulans down-stream of the opd gene. Southern analysis of restricted genomic DNA from various transformants indicated that integration occurred non-specifically at multiple sites. Western blot analysis of mycelial extracts from transformants confirmed the production of a processed form of the enzyme in the fungus. Maximal levels of OPH activity (rate of p-nitrophenol production from paraoxon) were observed after 168 h of culture and activity levels correlated with biomass production in mature vegetative growth.

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Section: Discussionmentioning

confidence: 75%

Expression of organophosphate hydrolase in the filamentous fungus Gliocladium virens

Dave

Lauriano

et al. 1994

Appl Microbiol Biotechnol

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“…Many studies concern pesticide degradation by Trichoderma spp., but the majority of them are conducted in culture media in laboratory conditions propitious for fungal growth. T. viride can degrade several organophosphorus (fenitrothion, fenitrooxon, parathion methyl), carbamate, and chlorinated hydrocarbon insecticides: DDT, endrin, aldrin [12,14,17,40]. In studies conducted by Katayama and Matsumura [13], T. harzianum, was found to degrade DDT, dieldrin, endosulfan, pentachloronitrobenzene, and pentachlorophenol, but not hexachlorocyclohexane.…”

Section: Discussionmentioning

confidence: 99%

Influence of a Commercial Biological Fungicide containing Trichoderma harzianum Rifai T-22 on Dissipation Kinetics and Degradation of Five Herbicides in Two Types of Soil

et al. 2020

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Biological crop protection is recommended to be applied alternately or together with chemical one, to protect human health from the excessive use of toxic pesticides. Presence of microorganisms can influence the concentration of chemical pollutants in soil. The aim of this study is to estimate the influence of a commercial biological fungicide containing Trichoderma harzianum Rifai T-22 on dissipation kinetics and degradation of five herbicides belonging to different chemical classes: clomazone, fluazifop-P-butyl, metribuzin, pendimethalin, and propyzamide, in two types of soil. Results of the study revealed that T. harzianum T-22 influences pesticide degradation and dissipation kinetics of the non-persistent herbicides: clomazone, fluazifop-P-butyl, and metribuzin. In soil with a higher content of nitrogen, phosphorus, and organic matter, degradation increased by up to 24.2%, 24.8%, and 23.5% for clomazone, fluazifop-P-butyl, and metribuzin, respectively. In soil with lower organic content, degradation was on a low level, of 16.1%, 17.7%, and 16.3% for clomazone, fluazifop-P-butyl, and metribuzin, respectively. In our study, the addition of the biological preparation shortened herbicide dissipation half-lives, from 0.3 days (2.9%) for fluazifop-P-butyl, to 18.4 days (25.1%) for clomazone. During the degradation study, no significant differences were noticed for pendimethalin, belonging to persistent substances. Biological protection of crops can modify pesticide concentrations and dissipation rates. On one hand, this may result in the reduced effectiveness of herbicide treatments, while on the other, it can become a tool for achieving cleaner environment.

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“…[24]Aspergillus niger JQ660373 [25], extracellular fungal hydrolases [26] has been proved efficient for the degradation of MCP. In addition to this other organophosphorus (OP) pesticides such as fenitrothion [phosphorothioic acid O,O-dimethyl-O-(3methyl-4-nitrophenyl) ester] has also been, known to be degraded by Trichoderma viride, MortierelIa isabeIIina, and Saprolegnia parasitica [27]. Bumpus et al, [28]had reported that P. chryososporium is able to degrade chlorpyrifos, fonofos, and terbufos to carbon dioxide.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Monocrotophos Hydrolyzing Soil Fungal Strain Penicillium aculeatum ITCC 7980.10

Jain¹,

Garg²

2015

IJEE

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Pesticides poses great threat to the environmental sustainability by affecting its microbial flora and fauna. Fungi are well known for the secretion of extracellular hydrolases for the degradation of these hazardous chemicals from its ambient environment. Hydrolase secreting soil fungi Penicillium aculeatum ITCC 7980.10 was selected for the study of its monocrotophos degrading efficiency under in vitro conditions in phosphorus free liquid culture medium. Fungal strain was molecularly characterized by 18S rDNA analysis as Penicillium aculeatum JQ660374. Monocrotophos degrading efficiency of the isolate was studied under optimum conditions each, at an interval of 5 days for 15 days incubation period. The isolated strain possessed meagre extracellular phosphatase activity 28.33 ± 0.40 U which resulted in the formation of 614.62 ± 0.38 µg ml-1 inorganic phosphates. Degradation process was studied by spectrophotometric analysis at 254nm, HPTLC and FTIR. Spectrophotometric analysis revealed 98.88% degradation of monocrotophos with a prominent decrease in the standard peak of monocrotophos (standard rf 0.19-0.21) within 15 days of incubation as evident by HPTLC chromatograms. Molecular mechanism as studied by FTIR analysis revealed hydrolytic cleavage of vinyl bond with the formation of PO-4. Degradation of monocrotophos followed first order kinetics with the rate constant (k deg) of 0.0115 day-1 and half life of 2.53 days. The results of the study conclude that Penicillium aculeatum JQ660374 could be used as an efficient candidate for the detoxification of monocrotophos contaminated sites.

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Biodegradation of fenitrothion and fenitrooxon by the fungus Trichoderma viride

Cited by 31 publications

References 6 publications

Expression of organophosphate hydrolase in the filamentous fungus Gliocladium virens

Expression of organophosphate hydrolase in the filamentous fungus Gliocladium virens

Influence of a Commercial Biological Fungicide containing Trichoderma harzianum Rifai T-22 on Dissipation Kinetics and Degradation of Five Herbicides in Two Types of Soil

Molecular Characterization of Monocrotophos Hydrolyzing Soil Fungal Strain Penicillium aculeatum ITCC 7980.10

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