Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp. strain Yw12

Abstract

Strain Yw12, isolated from activated sludge, could completely degrade and utilize methyl parathion as the sole carbon, phosphorus and energy sources for growth in the basic salt media. It could also completely degrade and utilize p-nitrophenol as the sole carbon and energy sources for growth in the minimal salt media. Phenotypic features, physiological and biochemical characteristics, and phylogenetic analysis of 16S rRNA sequence showed that this strain belongs to the genus of Agrobacterium sp. Response surface methodology was used to optimize degradation conditions. Under its optimal degradation conditions, 50 mg l(-1) MP was completely degraded within 2 h by strain Yw12 and the degradation product PNP was also completely degraded within 6 h. Furthermore, strain Yw12 could also degrade phoxim, methamidophos, chlorpyrifos, carbofuran, deltamethrin and atrazine when provided as the sole carbon and energy sources. Enzymatic analysis revealed that the MP degrading enzyme of strain Yw12 is an intracellular enzyme and is expressed constitutively. These results indicated that strain Yw12 might be used as a potential and effective organophosphate pesticides degrader for bioremediation of contaminated sites.

Hydrolysis of nicosulfuron under acidic environment caused by oxalate secretion of a novel Penicillium oxalicum strain YC-WM1

“…Sulfonylurea herbicides in natural environment can be removed via chemical hydrolysis 3–5 , microbial metabolism 6–8 , and photocatalytic degradation 9–18 . Microbes play an important role in the degradation of nicosufuron and numerous microbes isolated with the capacity of detoxifying have been proved to be one of the significant means to control environmental pollution.…”

Effects of Microcystin-LR on Metabolic Functions and Structure Succession of Sediment Bacterial Community under Anaerobic Conditions

“…Agrobacterium, is capable of degrading varieties of chemical substances, including organophosphorus herbicides and pesticides (atrazine, methyl parathion, etc. ), 3-hydroxypyridine, p-nitrophenol, and nicotine; and may be a potential MC-degrading bacterium [51][52][53][54].…”

Biodegradation of Chlorpyrifos (CP) by a Newly Isolated Naxibacter sp. Strain CY6 and Its Ability to Degrade CP in Soil

“…FDS-1, from the sludge of a wastewater treatment system in an OP manufacturing company. Recently, Wang et al (2012) isolated the MPT-degrading bacterium Agrobacgterium sp. Yw12 from activated sludge.…”

“…The CP or OP insecticides degrading bacteria was isolated previously (Sethunathan and Yoshida, 1973;Shelton, 1988;Rani and Lalitha-Kumari, 1994;Singh et al, 2004;Yang et al, 2005Yang et al, , 2006Zhang et al, 2005Zhang et al, , 2006Li et al, 2007;Xu et al, 2008;Anwar et al, 2009;Cho et al, 2009;Wang et al, 2012) but this is the first report of OP insecticides degradation by Naxibacter species. In addition, the several researchers has identified the various genes (opd) and enzymes (OP hydrolase) involved in degradation of OP insecticides (Fu et al, 2004;Liu et al, 2005;Zhang et al, 2006;Li et al, 2007;Yang et al, 2008;Islam et al, 2010).…”