Biodegradation of mixed polycyclic aromatic hydrocarbons by pure and mixed cultures of biosurfactant producing thermophilic and thermo-tolerant bacteria

Mehetre, Gajanan T.; Dastager, Syed G.; Dharne, Mahesh

doi:10.1016/j.scitotenv.2019.04.376

Cited by 106 publications

(28 citation statements)

References 73 publications

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“…Genetic and biochemical analyses about PAH-degrader of Gram-positive bacteria were also reported including thermophilic microorganisms [20,[26][27][28][29][30][31][32][33]. These facts imply that Gram-positive bacteria can play more important roles in the degradation of high molecular PAHs including biphenyl [34][35][36][37][38][39][40][41]. Further investigation including molecular analysis will provide us more clear information on naphthalene degradation including a lower pathway of thermophilic bacteria.…”

Section: Discussionmentioning

confidence: 94%

Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8

et al. 2019

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Geobacillus sp. JF8 is a thermophilic biphenyl and naphthalene degrader. To identify the naphthalene degradation genes, cis-naphthalene dihydrodiol dehydrogenase was purified from naphthalene-grown cells, and its N-terminal amino acid sequence was determined. Using a DNA probe encoding the N-terminal region of the dehydrogenase, a 10-kb DNA fragment was isolated. Upstream of nahB, a gene for dehydrogenase, there were two open reading frames which were designated as nahAc and nahAd, respectively. The products of nahAc and nahAd were predicted to be alpha and beta subunit of ring-hydroxylating dioxygenases, respectively. Phylogenetic analysis of amino acid sequences of NahB indicated that it did not belong to the cis-dihydrodiol dehydrogenase group that includes those of classical naphthalene degradation pathways. Downstream of nahB, four open reading frames were found, and their products were predicted as meta-cleavage product hydrolase, monooxygenase, dehydrogenase, and gentisate 1,2-dioxygenase, respectively. A reverse transcriptase-PCR analysis showed that transcription of nahAcAd was induced by naphthalene. These findings indicate that we successfully identified genes involved in the upper pathway of naphthalene degradation from a thermophilic bacterium.

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Section: Discussionmentioning

confidence: 94%

Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8

et al. 2019

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“…extreme, hydrocarboncontaminated terrestrial or marine environment [7] for biosurfactants production by B.licheniformis, Aeribacillus sp. Bacillus subtilis [13,27]. However, no previous report highlighted related to biosurfactant production by Bacillus nealsonii.…”

Section: Critical Micelles Concentration (Cmc) Determinationmentioning

confidence: 99%

“…The above analyses revealed that the biosurfactant produced by Bacillus nealsonii S2MT strain is a lipopeptide in nature having the highest resemblance with the surfactin family. In general, surfactin like biosurfactants relating to the family of cyclic lipopeptide and mostly produced by Bacillus spp [27]…”

mentioning

confidence: 99%

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Phulpoto

et al. 2020

Preprint

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Background Biosurfactants, being highly biodegradable, ecofriendly and multifunctional compounds have wide applications in various industrial sectors including environmental bioremediation. Surfactin a member of lipopeptide family which considered as one of the most powerful biosurfactant due to its environmental applications, emulsification activities as well as therapeutic properties. Therefore, the aim of this study to investigate the surfactin like biosurfactants produced by newly strain S2MT and their potential applications for soil remediation. Results In this study, a novel biosurfactant producing strain Bacillus nealsonii S2MT was investigated from the lake sediment that reduced the surface tension 34.15± 0.6 mN/m -1 with excellent emulsifying potential 55± 0.3% in kerosene oil. Additionally, the highest biosurfactant product 1300 mg/L -1 was achieved during which the composition of the culture medium was optimized through a response surface methodology (RSM). Results showed that 2% glycerol and 0.1% NH 4 NO 3 were the best carbon/nitrogen substrates for biosurfactant production. The most significant parameters such as temperature (30 °C), pH (8), agitation (100 rpm), NH 4 NO 3 (0.1%), yeast extract (0%) and NaCl (0.5%) contributed to the surface tension reduction and therefore enhancing the biosurfactant yield. Moreover, the obtained product was found to be highly stable at environmental factors such as salinity, pH and temperature variations. In addition, the biosurfactant product was chemically characterized as cyclic lipopeptide relating to surfactin-like isoforms (C 13 -C 15 ) with a thin-layer chromatography (TLC) and liquid chromatography and mass spectroscopy (LC/MS) respectively. The corresponding biosurfactant displayed 43.6± 0.08 and 46.7± 0.01% remediation of heavy engine-oil contaminated soil at 10 and 40 mg/L concentrations respectively. Conclusion This study, therefore, confirmed that the strain S2MT was not only a potential biosurfactant producer but also an efficient and environmentally acceptable candidate for soil remediation compared to synthetic compounds.

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“…9 This is particularly important because hydrocarbon-degrading extremophiles can tolerate a wide range of conditions and could be used for bioremediation of polluted extreme habitats. 10,11 There are four oxidation pathways of n-alkane degradation: (i) the monoterminal/terminal pathway where terminal methyl produces the primary alcohols further oxidized to fatty acid; 12 (ii) the biterminal pathway in which the termini of the n-alkane undergo oxidation to the corresponding fatty acids; 12 (iii) the subterminal pathway where the subterminal carbon is oxidized to an ester, 13 and; (iv) a unique pathway postulated for Acinetobacter sp. where n-alkanes are oxidized to fatty acids through n-alkyl hydroperoxides and aldehydes.…”

Section: Introductionmentioning

confidence: 99%

Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity

et al. 2020

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Biodegradation of mixed polycyclic aromatic hydrocarbons by pure and mixed cultures of biosurfactant producing thermophilic and thermo-tolerant bacteria

Cited by 106 publications

References 73 publications

Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8

Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity

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