Biodegradation of phenol by cold-adapted bacteria from Antarctic soils

Lee, Gillian Li Yin; Ahmad, Siti Aqlima; Yasid, Nur Adeela; Zulkharnain, Azham; Convey, Peter; Johari, Wan Lutfi Wan; Alias, Siti Aisyah; González-Rocha, Gerardo; Shukor, Mohd Yunus

doi:10.1007/s00300-017-2216-y

Cited by 48 publications

(62 citation statements)

References 56 publications

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“…The temperature optimization experiments carried out here confirmed that both strains were psychrotolerant, growing best at temperatures between 10°C and 15°C. This concurs with the findings of Shukor et al (2009), Margesin et al (2013) and Lee et al (2018).…”

Section: Discussionsupporting

confidence: 94%

“…Studies of non-polluted soils have also revealed psychrophilic or cold-tolerant members of the Arthrobacter genus to be important members of bacterial communities in Antarctic soils (Dsouza et al 2015). Margesin et al (2013) and Lee et al (2018) also reported Arthrobacter spp. strains capable of degrading petroleum hydrocarbons (n-alkanes and phenol) at low temperatures.…”

Section: Discussionmentioning

confidence: 99%

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Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

et al. 2020

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Bioremediation has been proposed as a means of dealing with oil spills on the continent. However, the introduction of non-native organisms, including microbes, even for this purpose would appear to breach the terms of the Environmental Protocol to the Antarctic Treaty. This study therefore aimed to optimize the growth conditions and diesel degradation activity of the Antarctic native bacteria Arthrobacter spp. strains AQ5-05 and AQ5-06 through the application of a one-factor-at-a-time (OFAT) approach. Both strains were psychrotolerant, with the optimum temperature supporting diesel degradation being 10–15°C. Both strains were also screened for biosurfactant production and biofilm formation. Their diesel degradation potential was assessed using Bushnell–Haas medium supplemented with 0.5% (v/v) diesel as the sole carbon source and determined using both gravimetric and gas chromatography and mass spectrophotometry analysis. Strain AQ5-06 achieved 37.5% diesel degradation, while strain AQ5-05 achieved 34.5% diesel degradation. Both strains produced biosurfactants and showed high biofilm adherence. Strains AQ5-05 and AQ5-06 showed high cellular hydrophobicity rates of 73.0% and 81.5%, respectively, in hexadecane, with somewhat lower values of 60.5% and 70.5%, respectively, in tetrahexadecane. Optimized conditions identified via OFAT increased diesel degradation to 41.0% and 47.5% for strains AQ5-05 and AQ5-06, respectively. Both strains also demonstrated the ability to degrade diesel in the presence of heavy metal co-pollutants. This study therefore confirms the potential use of these cold-tolerant bacterial strains in the biodegradation of diesel-polluted Antarctic soils at low environmental temperatures.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

et al. 2020

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“…Studies of non-polluted soils have also revealed psychrophilic or coldtolerant members of Arthrobacter to be important members of bacterial communities in Antarctic soils (Dsouza et al, 2015). Margesin et al (2013) and Lee et al (2018) also reported that Arthrobacter sp. strains are capable of degrading petroleum hydrocarbons (n-alkanes and phenol) at low temperature.…”

Section: Resultsmentioning

confidence: 99%

“…The isolates Arthrobacter sp. strains AQ5-05 and AQ5-06 used in this study were originally isolated from Antarctic soil obtained on King George Island, South Shetland Islands (Lee et al, 2018). The isolates were maintained in glycerol stock at a -80°C at Eco-Remediation Technology Laboratory, Department of Biochemistry, Faculty of Biotechnology and Science Biomolecules, Universiti Putra Malaysia until further use.…”

Section: Sample Collectionmentioning

confidence: 99%

Effects of heavy metals on diesel metabolism of psychrotolerant strains of Arthrobacter sp. from Antarctica

Abdulrasheed

Roslee

Zakaria

et al. 2020

JEB

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This present study aimed at examining the ability of cold-adapted Antarctic bacteria to tolerate and degrade diesel in the presence of different types of heavy metal co-pollutants. Arthrobacter sp. strains AQ5-05 and AQ5-06, originally isolated from Antarctic soils, were grown on Bushnell-Haas medium containing 1 ppm of heavy metal ions (As, Ag, Cd, Co, Cu, Cr, Hg, Ni, and Pb) supplemented with 3% (v/v) diesel. Diesel degradation was determined gravimetrically, while bacterial growth was evaluated by measuring the optical density of media (OD). 600 nm In the absence of heavy metal ions, strain AQ5-06 achieved 37.5% diesel mineralisation, while strain AQ5-05 achieved 34.5%. The diesel degrading abilities of both strains were significantly inhibited by exposure to < 1 ppm of Ag or Hg. In contrast, no change in degradation ability was observed using other tested heavy metals. The IC of Ag and Hg on 50 diesel degradation by the two strains were (0.2 and 0.4 ppm) and (0.3 and 0.2 ppm), respectively. Arthrobacter sp. Strains AQ5-05 and AQ5-06 may contain genes for alkane degradation and heavy metal resistance for remediating diesel-polluted soil in Antarctic and other cold regions. Antarctica, Arthrobacter sp., Biodegradation, Diesel, Heavy metals Effects of heavy metals on diesel metabolism of psychrotolerant strains of Arthrobacter sp. from Antarctica

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“…In this study, Rhodococcus erythropolis strain AQ5-07, a soil bacterium previously isolated from King George Island (South Shetland Islands, maritime Antarctic; [ 18 ]), was tested for its ability to degrade canola oil and produce biosurfactants, and its growth kinetics were studied and modelled. Canola oil is mostly used in research stations and is use in various cooking techniques, besides it degradation, Rhodococcus erythropolis strain AQ5-07 also could degrade phenol and diesel in addition to heavy metals resistance [ 3 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica

et al. 2020

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With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (qmax), concentration of substrate at the half maximum specific growth rate, Ks% (v/v) and the inhibition constant Ki% (v/v), with values of 0.142 h−1, 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions.

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Biodegradation of phenol by cold-adapted bacteria from Antarctic soils

Cited by 48 publications

References 56 publications

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

Effects of heavy metals on diesel metabolism of psychrotolerant strains of Arthrobacter sp. from Antarctica

Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica

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