Biodegradation of phenol by using free and immobilized cells of Acinetobacter sp. BS8Y

Abstract

Strain BS8Y with high biodegradation activity and high tolerance of phenol was isolated from activated sludge in an insulating material plant of China. This strain was capable of removing 99.2% of the initial 600 mg/l phenol in liquid minimal medium within 24 h and tolerating phenol at concentrations of up to 1,200 mg/ml. DNA sequencing and homologous analysis of the 16S rRNA gene identified that the strain BS8Y belonged to an Acinetobacter species. Polyvinyl alcohol was used as gel matrix to immobilize the strain BS8Y. The factors affecting the phenol degradation by immobilized cells and the phenol removal efficiency of free and immobilized cells were investigated; the stability of the immobilized cells is also reported. The results show that the immobilized cells could tolerate a higher phenol level and protected the bacteria much more effectively against changes in temperature and pH. The phenol degradation efficiency was high at up to 96% within 30 h, with an initial concentration of 800 mg/l phenol, and the immobilized cells showed better performance than the suspended cells. Reusability tests revealed that the immobilized cells were stable enough even after reuse for ten times or storing at 4°C for 35 d. These results demonstrate that immobilized Acinetobacter sp. BS8Y possesses a good application potential in the treatment of phenol-containing wastewater.

Phenolic Based Pharmaceutical Contaminated Wastewater Treatment Kinetics by Activated Sludge Process

“…At the end of the experiment, phenol was removed for 100.00 and 7.30% from the tests at the phenol concentrations of 10 and 100 mg/L, respectively. Some previous works found that bacterial cultures could use phenol as a growth substrate even up to concentrations of 10,000 mg/L [6], [9], [10]. In this study, based on the result of the test at the phenol concentration of 10 mg/L, it is clear that AS could degrade and use phenol as a sole carbon source similar to the previous work [6], [9], [10].…”

“…Some previous works found that bacterial cultures could use phenol as a growth substrate even up to concentrations of 10,000 mg/L [6], [9], [10]. In this study, based on the result of the test at the phenol concentration of 10 mg/L, it is clear that AS could degrade and use phenol as a sole carbon source similar to the previous work [6], [9], [10]. Additionally, some previous works reported phenol adsorption ability of AS [11], [12].…”

“…The previous works mostly focused on investigation of microbial cultures or systems to remove the phenolic compounds [2], [3], [5], [6]. To the best of our knowledge, no published work has been emphasized on inhibition of existing typical municipal activated sludge (AS) wastewater treatment influenced by phenolic based pharmaceuticals.…”

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“…cell leakage and metabolite exchange. The increased production of Daptomycin in immobilized system over free cells is accounted due to changes in permeability of cell wall as well as reusability of the matrices 15 . Immobilization capacity, time for immobilization, Daptomycin production with immobilized biomass and finally mechanical stability of the carrier are the key factors to be noted.…”

Bioremediation of Phenol-Contaminated Industrial Wastewater Using a Bacterial Consortium—from Laboratory to Field

“…(Adav et al 2007;Jiang et al 2013), Bacillus spp. (Banerjee and Ghoshal 2010;Kuang et al 2013) and Pseudomonas spp.…”