Biodegradation of phenol in refinery wastewater by pure cultures of Pseudomonas aeruginosa NCIB 950 and Pseudomonas fluorescence NCIB 3756

Agarry, S. E.; Durojaiye, A.O.; Yusuf, Rafiu Olasunkanmi; Aremu, M. O.; Solomon, B. O.; Mojeed, O.

doi:10.1504/ijep.2008.016894

Cited by 36 publications

(14 citation statements)

References 21 publications

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“…The K I value (90.24 mg/l) obtained in present study is higher than the K I value obtained for Pseudomonas aeruginosa (29.7 mg/l) and Pseudomonas fluorescence (31.7 mg/l). In contrary to present study, Agarry et al (2008) added nutrient medium to refinery wastewater to maintain the nutritional requirements for biomass growth. The biokinetic parameters suggest that diatom BD1IITG could prove to be an efficient strain for phenol removal from refinery wastewater due to the following characteristics: (a) High l max (0.4 day -1 ) value obtained (in refinery wastewater) indicates potential of the diatom for efficient biomass growth in refinery wastewater.…”

Section: Biomass Growth Phenol Degradation and Growth Kinetic Modelingmentioning

confidence: 81%

“…The K s value (20.99 mg/l) in refinery wastewater (first row in Table 2) is lower than that in nutrient media (first row in Table 1). A similar study was carried out for degradation of phenol in (Agarry et al 2008). The K I value (90.24 mg/l) obtained in present study is higher than the K I value obtained for Pseudomonas aeruginosa (29.7 mg/l) and Pseudomonas fluorescence (31.7 mg/l).…”

Section: Biomass Growth Phenol Degradation and Growth Kinetic Modelingmentioning

confidence: 96%

“…Microbial biodegradation rate is dependent on biomass growth rate. A higher biomass growth rate results in increased biodegradation rate (Agarry et al 2008). Thus, evaluation of microbial growth kinetics will bring forward its potential for biodegradation of organic compounds.…”

Section: Biomass Growth Phenol Degradation and Growth Kinetic Modelingmentioning

confidence: 99%

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Biodegradation of phenol by a novel diatom BD1IITG-kinetics and biochemical studies

Das

Mandal

Patra

2015

Int. J. Environ. Sci. Technol.

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A phenol-degrading novel diatom BD1IITG was isolated from petroleum refinery wastewater and characterized (GenBank Accession No. KJOO2533). HPLC analysis showed the diatom could degrade phenol in the concentration range of 50-250 mg/l in Fog's media. The highest specific growth and degradation rate were achieved at 100 mg/l phenol. It could also mineralize phenol along with aliphatics in petroleum refinery wastewater. Growth kinetic modeling shows that Haldane model best represents the growth behavior of the diatom in nutrient media as well as refinery wastewater. Biokinetic parameters suggest that the diatom possesses higher maximum specific growth rate (l max = 0.4 day -1 ), better tolerance to toxicity (K I = 90.24 mg/l) and high phenol affinity (K s = 20.99 mg/l) in refinery wastewater as compared to Fog's media confirming practical applicability of the strain for wastewater treatment. FTIR fingerprinting of biomass indicates intercellular phenol uptake and breakdown into its intermediates via phenol degradation pathway. Pathway was elucidated using HPLC, LC-MS and UV-visible spectrophotometry confirming prominence of ortho-over meta pathway for phenol metabolism. The diatom produces biosurfactant with highest emulsifying activity at 100 mg/l phenol which may contribute to highest degradation rate at this concentration. Infrared analysis confirms increased biosynthesis of lipids and polysaccharides in phenol-degrading biomass, indicating its potential use as feedstock of clean ecofriendly energy sources as biodiesel or bioethanol. The phenol degradation capability coupled with potential applicability of the spent biomass as biofuel feedstock makes diatom BD1IITG a potential candidate for a clean environmentally sustainable process. Graphical Abstract

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Section: Biomass Growth Phenol Degradation and Growth Kinetic Modelingmentioning

confidence: 81%

Section: Biomass Growth Phenol Degradation and Growth Kinetic Modelingmentioning

confidence: 96%

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Biodegradation of phenol by a novel diatom BD1IITG-kinetics and biochemical studies

Das

Mandal

Patra

2015

Int. J. Environ. Sci. Technol.

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“…Bacteria belonging to the genus Pseudomonas are metabolically adaptable, and able to degrade aromatic hydrocarbons, oil, petroleum products and pesticides (Otenio et al 2005;Abo-amer 2007;Kalyani et al 2008;Agarry et al 2008;Cycon et al 2009;Gautam and Sumathi 2009;Sarkar et al 2009;Toyama et al 2010). Pseudomonads are a diverse group of bacteria that occur in large numbers in the soil, where they are active in the mineralization of organic matter.…”

Section: Isolation and Characterization Of Cadusafos-degrading Bacteriamentioning

confidence: 99%

Characterization of a strain of Pseudomonas putida isolated from agricultural soil that degrades cadusafos (an organophosphorus pesticide)

Abo‐Amer

2011

World J Microbiol Biotechnol

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Bacteria capable of degrading the pesticide, cadusafos, were isolated from agricultural soil using an enrichment method. In this way, five distinct cadusafos-degrading strains of Pseudomonas putidia were isolated, and were characterized using morphological and biochemical analysis, as well as 16S rRNA sequencing. Strain PC1 exhibited the greatest cadusafos degradation rate and was consequently selected for further investigation. Degradation of cadusafos by strain PC1 was rapid at 20 and 37°C, but was greatly reduced (~1.5-fold) by the presence of carbon sources. Strain PC1 was able to effectively degrade cadusafos in sterilized soil using low inoculum levels. The maximum degradation rate of cadusafos (V ( max )) was calculated as 1.1 mg l(-1 )day(-1), and its saturation constant (K ( s )) was determined as 2.5 mg l(-1). Bacteria such as strain PC1, that use cadusafos as a carbon source, could be employed for the bioremediation of sites contaminated with pesticides.

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“…Phenolic compounds concentrate at the apices of elongation-inhibited radicals (Nun, Dadon, and Mayer 2005). P. fluorescens NCIB 3756 and P. aeruginosa NCIB 950 are known to biodegrade phenols (Agarry et al 2008) suggesting a role in the ability of P. aeruginosa to promote plant growth (Mansoor, Sultana, and Haque 2007). Injured pea roots are prompted to produce two phenolic compounds, gallic acid and naringenin, which inhibit GR24 induced germination of O. crenata (Mabrouk et al 2007a,b).…”

mentioning

confidence: 99%

Bacterial inhibition ofOrobanche aegyptiacaandOrobanche cernuaradical elongation

Barghouthi

Salman

2010

Biocontrol Science and Technology

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The ability of different bacteria to inhibit Orobanche radical elongation was examined. Seeds of Orobanche aegyptiaca Pers. and O. cernua Loefl. were induced to germinate with the Growth Regulator GR24 in the presence or absence of test bacterium. Radical lengths were estimated microscopically on a scale relative to seed length (0Á5 seed lengths) after 5 days of incubation at 258C. The results indicated that Pseudomonas aeruginosa QUBC1, P. fluorescens QUBC3, Bacillus atrophaeus QUBC16, and B. subtilis QUBC18 significantly inhibited radical elongation (P50.01) of both O. aegyptiaca and O. cernua relative to control radicals, whereas Microbacterium hydrocarbonoxydans QUBC11 and Ochrobactrum anthropi QUBC13 showed less inhibitory effects. Other bacterial isolates had no inhibitory effects. Bacterial isolates were identified using the universal method in addition to morphological and biochemical features. The establishment of the inhibitory effect of the most promising isolates, B. atrophaeus QUBC16 and P. aeruginosa QUBC1 on radical elongation of both Orobanche spp. is a step towards utilizing such bacteria as biocontrol agents against O. aegyptiaca, O. cernua, and potentially other Orobanche species.

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Biodegradation of phenol in refinery wastewater by pure cultures of Pseudomonas aeruginosa NCIB 950 and Pseudomonas fluorescence NCIB 3756

Cited by 36 publications

References 21 publications

Biodegradation of phenol by a novel diatom BD1IITG-kinetics and biochemical studies

Biodegradation of phenol by a novel diatom BD1IITG-kinetics and biochemical studies

Characterization of a strain of Pseudomonas putida isolated from agricultural soil that degrades cadusafos (an organophosphorus pesticide)

Bacterial inhibition ofOrobanche aegyptiacaandOrobanche cernuaradical elongation

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