Biodegradation of reactive textile dye Novacron Super Black G by free cells of newly isolated Alcaligenes faecalis AZ26 and Bacillus spp obtained from textile effluents

Abstract

Bacteria were isolated from effluents of textile industries and screened by their capability to decolorize at least one of eight reactive dyes used in the textile industries. Three isolates having the capability to decolorize the highest number of dyes with more than 25% of decolorization were identified as Alcaligenes faecalis AZ26, Bacillus cereus AZ27 and Bacillus sp. AZ28 based on morphological, cultural, biochemical characteristics, and 16S rDNA sequence analysis. The decolorization capability of these three bacterial isolates was optimized under different physicochemical conditions by using Novacron Super Black G (NSB-G), one of the eight reactive dyes commonly used in textile industries. These bacterial isolates grew well in the presence of up to 500 mg L −1 of NSB-G and showed decolorization of approximately 90% at 200 mg L −1 of NSB-G after 96 h of cultivation at 37 °C and pH 8.0 under static condition. Decolorization of NSB-G by the bacterial isolates was investigated using UV-VIS spectrophotometry and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The UV-visible absorbance spectra and the FTIR spectrum of the decolorized NSB-G significantly differed from those of the parent dye, indicating that the NSB-G was degraded by the bacterial isolates. High decolorization extent supports the notion that the bacterial isolates reported herein might have potential in the biological treatment of dyeing mill effluents.

Isolation, characterization, and decolorization of Disperse Blue 60 by newly isolated bacterial strains from Kashan textile wastewater

“…Gulati and Jha isolated two bacterial strains from dye effluent contaminated the soil with degrading ability of Reactive Blue 19 dye, up to 95% after 72 hr in optimum conditions (Gulati & Jha, ). Hossen et al, have similar results and indicated that the decolorization of NSB‐G dye by the three bacterial isolates was almost saturated (∼90%) after 72 hr of incubation (Hossen et al, ).…”

Current Trends on Role of Biological Treatment in Integrated Treatment Technologies of Textile Wastewater

“…Although good results are achieved in terms of reducing the chemical oxygen demand (COD) and decolorization, usually these values are for dye concentration that does not exceed the 200 mg L –1 , and as was shown earlier dye concentration in real textile wastewater can reach until 800 mg L –1 . In addition, it should be noted that reactor volumes in many cases are small ( Valli Nachiyar et al, 2016 ; Abd El-Rahim et al, 2017 ; Hossen et al, 2019 ; Zahran et al, 2019 ). As an example, Hossen et al (2019) studied the decolorization efficiency of different strains in simulated effluents, some of which reached efficiencies of even 90%.…”

Textiles wastewater treatment technology: A review

“…The 16S rDNA sequencing analysis has been used, and the authors concluded bacterial isolate TS20 was the most predominant bacteria species for color removal. Hossen et al (2019) investigated the remediation of Novacron Super Black G dye (NSB-G) using three bacteria isolates (Bacillus sp. AZ28, Bacillus cereus AZ27, and Alcaligenes faecalis AZ26) from textile effluent.…”

“…Hossen et al (2019) investigated the remediation of Novacron Super Black G dye (NSB‐G) using three bacteria isolates ( Bacillus sp. AZ28, Bacillus cereus AZ27, and Alcaligenes faecalis AZ26) from textile effluent.…”