Biodegradation of the major color containing compounds in distillery wastewater by an aerobic bacterial culture and characterization of their metabolites

Abstract

This study deals the biodegradation of the major color containing compounds extracted from distillery wastewater (DWW) by an aerobic bacterial consortium comprising Bacillus licheniformis (DQ79010), Bacillus sp. (DQ779011) and Alcaligenes sp. (DQ779012) and characterization of metabolic products. The degradation of color containing compounds by bacteria was studied by using the different carbon and nitrogen sources at different environmental conditions. Results revealed that the bacterial consortium was efficient for 70% color removal in presence of glucose (1.0%) and peptone (0.1%) at pH 7.0 and temperature 37 degrees C. The HPLC analysis of control and bacterial degraded samples has shown the reduction in peak area as well as shifting of peaks compared to control indicating the bacterial degradation as well as transformation of color containing compounds from DWW. The comparative LC-MS-MS and other spectrophotometric analysis has shown the presence of dihydroxyconiferyl alcohol, 2, 2'-bifuran-5-carboxylic acid, 2-nitroacetophenone, p-chloroanisol, 2, 3-dimethyl-pyrazine, 2-methylhexane, methylbenzene, 2, 3-dihydro-5-methylfuran, 3-pyrroline, and acetic acid in control samples that were biodegraded and biotransformed into 2-nitroacetophenone, p-chloroanisol, 2, 2'-bifuran, indole, 2-methylhexane, and 2, 3-dihydro-5-methylfuran by bacterial consortium. In this study, it was observed that most of the compounds detected in control samples were diminished from the bacterial degraded samples and compounds 2, 2'-bifuran and indole with molecular weight 134 and 117 were produced as new metabolites during the bacterial degradation of color containing compounds from DWW.

Decolourization of Sugar Beet Molasses Vinasse by Lactic Acid Bacteria – the Effect of Yeast Extract Dosage

“…(DQ779011), and Alcaligenes sp. (DQ779012) used for decolourization by Bharagava and Chandra [28], the use of the additive of organic nitrogen source (peptone) in an amount greater than 1 g dm -3 , as in our studies after increasing the dose (more than 2.24 g dm -3 ), contributed to a reduction in the degree of removal of colour. Yadav and Chandra [20] also reported that doses of more than 1 g dm -3 of organic nitrogen source showed an inhibitory effect on the decolourization process.…”

Characterisation of manganese peroxidase and laccase producing bacteria capable for degradation of sucrose glutamic acid-Maillard reaction products at different nutritional and environmental conditions

“…Melanoidins are complex, dark coloured, amino-carbonyl polymeric compounds with high molecular weight, formed by non-enzymatic browning Maillard reaction (MR) during several industrial processes (Hayase 2000 ; Echavarría et al 2014 ; Bharagava and Chandra 2010 ). The major sources of melanoidins in environment are sugarcane-molasses based distilleries and fermentation industries all over the world.…”

Color removal of melanoidin-rich industrial effluent by natural manganese oxides

“…Various methods have been tried to remove melanoidin color and recalcitrant COD. Biological processes employing bacteria [9], fungi [10], and enzyme [11] have been tried before to treat melanoidin-rich wastewater. Though biological processes are generally less costly than chemical or physical treatment methods, none of the reported methods can effectively remove the contaminants in melanoidin-rich wastewaters.…”