Biodegradation of waste cooking oil and simultaneous production of rhamnolipid biosurfactant by Pseudomonas aeruginosa P7815 in batch and fed-batch bioreactor

Sharma, Swati; Verma, Rajan; Dhull, Sahil; Maiti, Soumen K.; Pandey, Lalit M.

doi:10.1007/s00449-021-02661-0

Cited by 24 publications

(4 citation statements)

References 44 publications

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“…Usually, complex or multicomponent media is used to produce biosurfactants [ 17 , 18 ], but in the present study, a simple media of peptone water was the base for biosurfactant production. Peptone was reported to be a suitable organic nitrogen source for biosurfactant since the amino acids present buffer the pH and therefore maintain the lipase activity which was reported to aid in emulsification of oil throughout the production process [ 7 ]. Carbon source and inoculum size were chosen as two key factors that control Serratia marcescens N2 biosurfactant production.…”

Section: Discussionmentioning

confidence: 99%

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Valorization of frying oil waste for biodetergent production using Serratia marcescens N2 and gamma irradiation assisted biorecovery

Elkenawy

Gomaa

2022

Microb Cell Fact

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Background The complexity, toxicity and abundance of frying oil waste (FOW) render it difficult to be degraded biologically. The aim of the present work was to valorize FOW and investigate the potential use of the produced biosurfactant by Serratia marcescens N2 (Whole Genome sequencing accession ID SPSG00000000) as a biodetergent. Results Serratia marcescens N2 demonstrated efficient valorization of FOW, using 1% peptone, 20% FOW and 8% inoculum size. Gene annotation showed the presence of serrawettin synthetase indicating that the produced biosurfactant was serrawettin. Zeta potential and Fourier Transform Infrared (FTIR) spectroscopy indicate that the biosurfactant produced was a negatively charged lipopeptide. The biosurfactant reduced the surface tension of water from 72 to 25.7 mN/m; its emulsification index was 90%. The valorization started after 1 h of incubation and reached a maximum of 83.3%. Gamma radiation was used to increase the biosurfactant yield from 9.4 to 19.2 g/L for non-irradiated and 1000 Gy irradiated cultures, respectively. It was noted that the biorecovery took place immediately as opposed to overnight storage required in conventional biosurfactant recovery. Both chemical and functional characteristics of the radiation induced biosurfactant did not change at low doses. The produced biosurfactant was used to wash oil stain; the highest detergency reached was 87% at 60 °C under stirring conditions for 500 Gy gamma assisted biorecovery. Skin irritation tests performed on experimental mice showed no inflammation. Conclusion This study was able to obtain a skin friendly effective biodetergent from low worth FOW using Serratia marcescens N2 with 83% efficient valorization using only peptone in the growth media unlike previous studies using complex media. Gamma radiation was for the first time experimented to assist biosurfactant recovery and doubling the yield without affecting the efficiency.

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Section: Discussionmentioning

confidence: 99%

“…FOW is commonly recycled into other value-added products such as soap, a very simple transformation that is attracting the attention of households and start-up companies in Egypt. Using microorganisms to utilize and transform FOW and convert it to value-added product such as biosurfactant is another attractive solution [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Valorization of frying oil waste for biodetergent production using Serratia marcescens N2 and gamma irradiation assisted biorecovery

Elkenawy

Gomaa

2022

Microb Cell Fact

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“…Furthermore, negligible effects on Escherichia coli MTCC723 of 3.6% and 4.4%, respectively, were observed for both sophorolipids. According to [ 99 ], rhamnolipids present a minimum inhibitory concentration ranging 50–1600 µg mL −1 . Higher biosurfactant concentrations are needed against gram-negative bacteria because of their trick lipopolysaccharide outer membrane.…”

Section: Environmental Uses Of Biosurfactantsmentioning

confidence: 99%

Microbial biosurfactants: a review of recent environmental applications

et al. 2022

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Microbial biosurfactants are low-molecular-weight surface-active compounds of high industrial interest owing to their chemical properties and stability under several environmental conditions. The chemistry of a biosurfactant and its production cost are defined by the selection of the producer microorganism, type of substrate, and purification strategy. Recently, biosurfactants have been applied to solve or contribute to solving some environmental problems, with this being their main field of application. The most referenced studies are based on the bioremediation of contaminated soils with recalcitrant pollutants, such as hydrocarbons or heavy metals. In the case of heavy metals, biosurfactants function as chelating agents owing to their binding capacity. However, the mechanism by which biosurfactants typically act in an environmental field is focused on their ability to reduce the surface tension, thus facilitating the emulsification and solubilization of certain pollutants ( in-situ biostimulation and/or bioaugmentation). Moreover, despite the low toxicity of biosurfactants, they can also act as biocidal agents at certain doses, mainly at higher concentrations than their critical micellar concentration. More recently, biosurfactant production using alternative substrates, such as several types of organic waste and solid-state fermentation, has increased its applicability and research interest in a circular economy context. In this review, the most recent research publications on the use of biosurfactants in environmental applications as an alternative to conventional chemical surfactants are summarized and analyzed. Novel strategies using biosurfactants as agricultural and biocidal agents are also presented in this paper.

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“…Therefore, the reclaim and reuse of WCO can effectively address the scarcity of petroleum resources, reduce environmental pollution, and safeguard public health. [1][2][3] Although there are numerous ways to repurpose WCO and it can produce a variety of products like biofuels (biodiesel, 4-7 bioethanol [8][9][10] and biogas 11,12 ), biolubricants, 13-15 biosurfactants, [16][17][18] asphalt modier, [19][20][21][22] washing product, 23,24 etc., traditional products based on WCO oen exhibit quite a low-prot margin (oen <20%) and necessitate large-scale production lines and signicant investments. For instance, to produce biodiesel from WCO, rst a stable and optimal supply chain of raw materials must be established, requiring strong policy and nancial support.…”

Section: Introductionmentioning

confidence: 99%

Fatty acid wax from epoxidation and hydrolysis treatments of waste cooking oil: synthesis and properties

et al. 2022

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Biodegradation of waste cooking oil and simultaneous production of rhamnolipid biosurfactant by Pseudomonas aeruginosa P7815 in batch and fed-batch bioreactor

Cited by 24 publications

References 44 publications

Valorization of frying oil waste for biodetergent production using Serratia marcescens N2 and gamma irradiation assisted biorecovery

Valorization of frying oil waste for biodetergent production using Serratia marcescens N2 and gamma irradiation assisted biorecovery

Microbial biosurfactants: a review of recent environmental applications

Fatty acid wax from epoxidation and hydrolysis treatments of waste cooking oil: synthesis and properties

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