Biodegradation time series characteristics and metabolic fate of different aromatic compounds in the biochemical treatment process of coal chemical wastewater

Yang, Lü; Liu, Yongjun; Li, Chen; Liu, Zhe; Liu, Xingshe; Wei, Chunhong; Yang, Zhuangzhuang; Zhang, Aining

doi:10.1016/j.biortech.2022.127688

Cited by 37 publications

(2 citation statements)

References 42 publications

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“…EPS can modulate cell surface charge and increase hydrophobicity (42) by virtue of the composition and abundance of proteins and lipids, and thus may increase the local benzene concentration in the surrounding environment of cell aggregates via diffusion or absorption (33,37). Yang et al (43) measured higher benzene concentrations in the EPS of biosludge which may be similar to our ORM2 cell aggregates. When bulk benzene aqueous concentrations are low, the higher local concentrations afforded by cell aggregation and EPS production could benefit the growth of ORM2.…”

Section: Microscopic Images Reveal Cell Aggregationsupporting

confidence: 65%

Visualization of syntrophic benzene-fermentingDesulfobacterotaORM2 in a methanogenic enrichment culture using fluorescencein situhybridization

Chen

Toth

Guo

et al. 2023

Preprint

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Although benzene degradation under strictly anoxic conditions was first reported over 25 years ago, the mechanism for benzene activation in the absence of oxygen is still elusive. A major limitation has been the difficulty to grow anaerobic benzene-degrading enrichment cultures. Our laboratory has maintained a methanogenic enrichment culture for decades, harboring a benzene fermenter referred to as Desulfobacterota ORM2. Recent genomic analyses indicate that ORM2 is not affiliated with any characterized genus, but it is phylogenetically similar to several other known and predicted benzene degraders. Desulfobacterota ORM2 has a doubling time of approximately 30 days and often enters a long lag or decay phase after inoculation into sterile pre-reduced anaerobic medium. A specific fluorescent in situ hybridization (FISH) probe was used to observe Desulfobacterota ORM2 cells during this decay phase, revealing a rod-shaped cell of variable length with a tendency to associate with other cells, particularly methanogens. Microscopic and genomic analyses indicate that Desulfobacterota ORM2 may produce extracellular polymeric substances (EPS) that likely contribute to cell aggregation. The production of EPS may consume a significant amount of energy, perhaps contributing to the lag time before onset of growth of Desulfobacterota ORM2 post-inoculation. We observed little cell aggregation in a culture amended with very high concentrations of benzene (90-120 mg/L). This study visualized the cells of a novel clade within the Desulfobacterota for the first time, enabling monitoring of spatial organization within a methanogenic consortium and provides hints to improve the growth rate of ORM2.

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Section: Microscopic Images Reveal Cell Aggregationsupporting

confidence: 65%

Visualization of syntrophic benzene-fermentingDesulfobacterotaORM2 in a methanogenic enrichment culture using fluorescencein situhybridization

Chen

Toth

Guo

et al. 2023

Preprint

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show abstract

“…The detection of 16 PAHs in 59 sediment samples was performed by gas chromatography (Agilent 7890a) according to the method of Yang et al (Yang et al, 2022). The HP-5 chromatographic column was selected for the PAHs.…”

Section: Extraction and Component Analysis Of Pahsmentioning

confidence: 99%

Distribution Characteristics, Source Analysis and Risk Assessment of Polycyclic Aromatic Hydrocarbons in Sediments of Kuye River: A River in a Typical Energy and Chemical Industry Zone

Liu

Zhang

Yang

et al. 2023

Preprint

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Distribution, sources, and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in Kaokaowusu river sediments near a coal industrial zone

Wang

Liu

Yang

et al. 2022

Environ Geochem Health

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Biodegradation time series characteristics and metabolic fate of different aromatic compounds in the biochemical treatment process of coal chemical wastewater

Cited by 37 publications

References 42 publications

Visualization of syntrophic benzene-fermentingDesulfobacterotaORM2 in a methanogenic enrichment culture using fluorescencein situhybridization

Visualization of syntrophic benzene-fermentingDesulfobacterotaORM2 in a methanogenic enrichment culture using fluorescencein situhybridization

Distribution Characteristics, Source Analysis and Risk Assessment of Polycyclic Aromatic Hydrocarbons in Sediments of Kuye River: A River in a Typical Energy and Chemical Industry Zone

Distribution, sources, and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in Kaokaowusu river sediments near a coal industrial zone

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