Biodehalogenation: The metabolism of vinyl chloride by <i>Methylosinus trichosporium</i> OB‐3b. A sequential oxidative and reductive pathway through chloroethylene oxide

Castro, C. E.; Riebeth, D.M.; Belser, N. O.

doi:10.1002/etc.5620110604

Cited by 25 publications

(1 citation statement)

References 15 publications

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“…First, methane generated in the anaerobic source area that migrates with (or somewhat ahead of) the daughter products can be used as a primary substrate for the cometabolism of VC and cDCE under aerobic conditions downgradient. Both VC and cDCE are readily consumed by methanotrophs (6)(7)(8)(9)(10)(11)(12)(13). Second, ethene and ethane formed from reductive dechlorination of VC in the anaerobic zone can be used as primary substrates for the cometabolism of cDCE (14) and VC (14)(15)(16)(17)(18) under aerobic conditions.…”

Section: Introductionmentioning

confidence: 99%

Cometabolism of cis-1,2-Dichloroethene by Aerobic Cultures Grown on Vinyl Chloride as the Primary Substrate

Verce

Gunsch

Danko

et al. 2002

Environ. Sci. Technol.

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An aerobic enrichment culture was grown on vinyl chloride (VC) as the sole source of carbon and energy. In the absence of VC, the enrichment culture cometabolized cis-1,2-dichloroethene (cDCE) and, to a lesser extent, trans1,2-dichloroethene (tDCE), beginning with oxidation to the corresponding DCE-epoxides. When provided with VC (1.3 mM) and cDCE (0.2-0.3 mM), the enrichment culture cometabolized repeated additions of cDCE for over 85 days. Cometabolism of repeated additions of tDCE was also demonstrated but at a lower ratio of nongrowth substrate to VC. VC-grown Pseudomonas aeruginosa MF1 (previously isolated from the enrichment culture) also readily cometabolizes cDCE, with an observed transformation capacity (Tc,obs) of 0.82 micromol of cDCE/mg of total suspended solids (TSS). When provided with VC and cDCE, MF1 did not begin cometabolizing cDCE until nearly all of the VC was consumed. The presence of cDCE reduces the maximum specific rate of VC utilization. A kinetic model was developed that describes these phenomena via Monod parameters for substrate and nongrowth substrate, plus inactivation and inhibition coefficients. MF1 did not show any cometabolic activity on tDCE or trichloroethene and very limited activity on 1,1-DCE (Tc,obs = 2 x 10(-5) micromol/mg TSS). Above 40 microM, tDCE and TCE noticeably increased the maximum specific rate of VC utilization, even though neither compound was consumed during or after VC consumption. High concentrations of 1,1-DCE (950 microM) completely inhibited VC biodegradation. As there is currently no evidence for aerobic biodegradation of cDCE as a sole source of carbon and energy, the results of this study provide a potential explanation for in situ disappearance of cDCE when the only other significant substrate available is VC. It is fortuitous that the VC-grown cultures tested exhibit their highest cometabolic activity toward cDCE, because it is the predominant DCE isomer formed during anaerobic reductive dechlorination of trichloroethene and tetrachloroethene.

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Section: Introductionmentioning

confidence: 99%

Cometabolism of cis-1,2-Dichloroethene by Aerobic Cultures Grown on Vinyl Chloride as the Primary Substrate

Verce

Gunsch

Danko

et al. 2002

Environ. Sci. Technol.

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Modeling the kinetics of vinyl chloride cometabolism by an ethane-grownPseudomonas sp.

Verce

Freedman

2000

Biotechnol. Bioeng.

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Dechlorination of TetrachloroetheneInfluence of Tetrachloroethene and Methane in Batch Cultivation under Methanotrophic Conditions in the Presence of Oxygen

et al. 2001

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The feasibility of combining the reductive dechlorination and oxidative mineralization of tetrachloroethene (PCE) within one system was investigated in non-shaken methanotrophic batch cultivated cell suspensions. In the presence of up to 90% [v/v] methane and 10% [v/v] oxygen in the headspace, 200, 400 and 500 µmol/l PCE were completely dechlorinated over 100 days of incubation with 1% [v/v] activated sludge derived from a municipal wastewater treatment plant (equivalent to 6 mmol/l carbon). Meanwhile nearly 4 mol/l chloride were released to the medium for each mol PCE dechlorinated, indicating that the volatile chlorinated compound was completely dechlorinated in the cell suspensions. Irrespectively of the initial PCE concentration, 40 µmol/l trichloroethene were found temporarily. According to the initial PCE concentration, up to 160 µmol/l cis-dichloroethene were measured in the assays with 500 µmol/l PCE. In the presence of oxygen in the headspace, simultaneously with the dechlorination, methane was oxidized. At 7% [v/v] methane and 10% [v/v] oxygen, 500 µmol/l PCE were dechlorinated to cDCE within 145 days. At PCE concentrations of 600 µmol/l or above, substrate inhibition occurred, and then both the dechlorination and the oxidation of methane were inhibited.

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Biodehalogenation: The metabolism of vinyl chloride by Methylosinus trichosporium OB‐3b. A sequential oxidative and reductive pathway through chloroethylene oxide

Cited by 25 publications

References 15 publications

Cometabolism of cis-1,2-Dichloroethene by Aerobic Cultures Grown on Vinyl Chloride as the Primary Substrate

Cometabolism of cis-1,2-Dichloroethene by Aerobic Cultures Grown on Vinyl Chloride as the Primary Substrate

Modeling the kinetics of vinyl chloride cometabolism by an ethane-grownPseudomonas sp.

Dechlorination of TetrachloroetheneInfluence of Tetrachloroethene and Methane in Batch Cultivation under Methanotrophic Conditions in the Presence of Oxygen

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