Biodetection and bioremediation of copper ions in environmental water samples using a temperature-controlled, dual-functional Escherichia coli cell

Development of a Sensitive Escherichia coli Bioreporter Without Antibiotic Markers for Detecting Bioavailable Copper in Water Environments

“…(A) The copAp::gfpmut2-pET28a/ cusA-copA-cueO (E. coli WMC-006) bioreporters induced by a series of copper concentration (numbers 2-8: 0, 1.0 × 10 −8 , 1.0 × 10 −8 , 1.0 × 10 −7 , 1.0 × 10 −6 , 1.0 × 10 −5 , 1.0 × 10 −4 , and 1.0 × 10 tested their sensitivity to copper ions. As shown in Figures 2C-E, 1 mM copper ions induced weak fluorescence in bioreporter based on wild type of E. coli MC4100, as previously reported (Kang et al, 2018;Wang et al, 2019). The IC values suggested that the effect of copper transport genes on the sensitivity of these whole-cell bioreporters was ranked: copA > cueO > cusA (Figures 2C,D).…”

“…coli copA (Figure 2E). Similarly, our previous work showed that the fluorescent signal generated by the copper-sensing element in E. coli copA cueO was significantly stronger than that of E. coli copA (Wang et al, 2019). Therefore, the mutant strain E. coli copA cueO cusA was selected as the host strain to construct a new bioreporter by inserting the copAp::gfpmut2 sensing element into its chromosome.…”

“…coli copA cueR bioreporter. Our previous work reported a dual-functional E. coli cell that could detect and remove copper ions, which selectively responded to copper ions with a linear detection range of 0.01-25 µM (Wang et al, 2019).…”

A simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease

“…Surface-anchoring motifs, including OmpA, ice nucleation protein (INP) (Li et al 2004), and auto-transporters (van Ulsen et al 2018), act as carriers to deliver the target proteins across the bacterial cell membrane. Among them, Lpp-OmpA was frequently used (Fasehee et al 2018;Wang et al 2019;Wei et al 2014) and was considered an ideal carrier protein (Lang 2000). For example, E. coli cells expressing Lpp-OmpAanchored EC20 (a metal-binding protein) accumulated substantially more mercury than did cells engineered with INP-fused EC20 (Bae et al 2002).…”

Preparation, Performances, and Mechanisms of Microbial Flocculants for Wastewater Treatment

“…When we enjoy the convenience they bring to the development of all walks of life, we must also pay attention to the environmental damage and the threat to the health of living organisms during the production process. Bioremediation is the use of living organisms, or part of it, or their metabolites, for the recovery or cleaning up of a contaminated medium such as soil [1,2], sediment [3,4], or liquid/water [5,6]. With the growth of population and the development of science and technology, more water is consumed in daily life and production, and more wastewater is generated.…”