Biodiscovery of aluminum binding peptides

Abstract

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Selective preference of engineered peptides for aluminum alloy

“…[27][28][29][30] We have previously utilized this library to discover peptide binders for several protein targets, such as Anthrax toxin component protective antigen 30,31 and the Staphylococcal enterotoxin B, 32 as well as peptide binders for bulk aluminum. 10,33 We found that E. coli displaying peptide DBAD1 bound well to the surface of bulk aluminum, which is expected to possess a natural oxide (alumina layer). Moreover, through a combination of sequence analysis, modeling and simulation, we showed evidence that the DBAD1 peptide possesses a high helical propensity due to the presence of alanine residues which facilitate the presentation of hydroxyl-containing residues for interaction with the bulk aluminum surface.…”