Selective preference of engineered peptides for aluminum alloy

Adams, Bryn L.; Stratis‐Cullum, Dimitra N.

doi:10.1680/emr.15.00028

Cited by 3 publications

(3 citation statements)

References 40 publications

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“…26,57,58,67,78 It is clear from Fig. 3 that all three peptides are lacking in helical propensity and avoid any overwhelming structural characteristics which could interfere in the binding process.…”

Section: Comparison Of Peptide Binding To Goldmentioning

confidence: 99%

“…23 It was also used to demonstrate the selectivity of an aluminum alloybinding peptide developed from the eCPX library, for its target alloy over glass, copper or brass. 67 Regardless of the peptide display library type or host organisms, the key advantage to all biocombinatorial approaches is that the peptide's identity is encoded by the organism's genetic material. Therefore, a direct physical linkage exists between the displayed peptide and the host's DNA, allowing the amino acid composition to be derived through DNA sequencing analysis.…”

Section: Surface Display Scaffoldsmentioning

confidence: 99%

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Functional and Selective Bacterial Interfaces Using Cross-Scaffold Gold Binding Peptides

Adams

Hurley

Jahnke

et al. 2015

JOM

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We investigated the functional and selective activity of three phage-derived gold-binding peptides on the Escherichia coli (E. coli) bacterial cell surface display scaffold (eCPX) for the first time. Gold-binding peptides, p3-Au12 (LKAHLPPSRLPS), p8#9 (VSGSSPDS), and Midas-2 (TGTSVLIATPYV), were compared side-by-side through experiment and simulation. All exhibited strong binding to an evaporated gold film, with approximately a 4-log difference in binding between each peptide and the control sample. The increased affinity for gold was also confirmed by direct visualization of samples using Scanning Electron Microscopy (SEM). Peptide dynamics in solution were performed to analyze innate structure, and all three were found to have a high degree of flexibility. Preferential binding to gold over silicon for all three peptides was demonstrated, with up to four orders of magnitude selectivity exhibited by p3-Au12. The selectivity was also clearly evident through SEM analysis of the boundary between the gold film and silicon substrate. Functional activity of bound E. coli cells was further demonstrated by stimulating filamentation and all three peptides were characterized as prolific relative to control samples. This work shows great promise towards functional and active bacterial-hybrid gold surfaces and the potential to enable the next generation living material interfaces.

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Section: Comparison Of Peptide Binding To Goldmentioning

confidence: 99%

Section: Surface Display Scaffoldsmentioning

confidence: 99%

Functional and Selective Bacterial Interfaces Using Cross-Scaffold Gold Binding Peptides

Adams

Hurley

Jahnke

et al. 2015

JOM

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“…The next paper 7 in this issue focuses on the 'Selective preference of engineered peptides for aluminum alloy'. In this paper by Adams and Stratis-Cullum of the US Army Research Laboratory, Adelphi, Maryland, USA, the authors discuss bacterial cell-surface display technology as a powerful tool for the discovery and study of peptide material interactions offering the potential toward tailoring the interaction and tuning the properties of multicomponent materials for future military and commercial systems.…”

mentioning

confidence: 99%

Editorial

Ravindra¹

2015

Emerging Materials Research

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“Living” dynamics of filamentous bacteria on an adherent surface under hydrodynamic exposure

et al. 2017

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The potential advantages of cell-based biohybrid devices over conventional nonliving systems drive the interest to control the behavior of the underlying biological cells in microdevices. Here, the authors studied how shear influenced the geometry and elongation of fimbriated filaments on affinity substrates. The cells were engineered to express FimH, which binds to mannose with a high affinity. A microfluidic channel was functionalized with RNAse B, which is rich in mannose residues, and the device was used to control the hydrodynamic force on live Escherichia coli under filamentous growth. It was discovered that filamentous E. coli cells adopt buckled geometry when the shear rate is low, but assume an extended geometry at high shear and align with the flow direction. The extension moves from bidirectional to preferentially downstream as the shear rate increases. Furthermore, living filaments slide easily on the substrate, and detach from the substrates at a rate nearly ten times greater than unfilamented live E. coli at high shear conditions (1000-4000 s). The hydrodynamic force and binding force experienced by the cells are further analyzed by COMSOL simulation and atomic force microscopy measurements, respectively, to explore the mechanism behind the living cell dynamics. Knowledge from this work helps guide design of interfacial properties and shear environments to control the geometry of living filamentous bacteria.

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Selective preference of engineered peptides for aluminum alloy

Cited by 3 publications

References 40 publications

Functional and Selective Bacterial Interfaces Using Cross-Scaffold Gold Binding Peptides

Functional and Selective Bacterial Interfaces Using Cross-Scaffold Gold Binding Peptides

Editorial

“Living” dynamics of filamentous bacteria on an adherent surface under hydrodynamic exposure

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