Biodistribution of ultra small superparamagnetic iron oxide nanoparticles in BALB mice

Shanehsazzadeh, Saeed; Oghabian, Mohammad Ali; Daha, Fariba Johari; Amanlou, Massoud; Allen, Barry J.

doi:10.1007/s10967-012-2173-4

Cited by 34 publications

(40 citation statements)

References 29 publications

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“…As anticipated from previous studies on USPIO biodistribution, there was a strong uptake of FLUSPIO by organs of the reticuloendothelial systems, in particular the liver and spleen [33,34]. Further, its accumulation in the lung can be explained by macrophage uptake.…”

Section: Bio-distribution Of Fluspiosupporting

confidence: 60%

In vivo evaluation of riboflavin receptor targeted fluorescent USPIO in mice with prostate cancer xenografts

et al. 2016

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Riboflavin (Rf) receptors bind and translocate Rf and its phosphorylated forms (e.g. flavin mononucleotide, FMN) into cells where they mediate various cellular metabolic pathways. Previously, we showed that FMN-coated ultrasmall superparamagnetic iron oxide (FLUSPIO) nanoparticles are suitable for labeling metabolically active cancer and endothelial cells in vitro. In this study, we focused on the in vivo application of FLUSPIO using prostate cancer xenografts. Size, charge, and chemical composition of FLUSPIO were evaluated. We explored the in vitro specificity of FLUSPIO for its cellular receptors using magnetic resonance imaging (MRI) and Prussian blue staining. Competitive binding experiments were performed in vivo by injecting free FMN in excess. Bio-distribution of FLUSPIO was determined by estimating iron content in organs and tumors using a colorimetric assay. AFM analysis and zeta potential measurements revealed a particulate morphology approximately 20-40 nm in size and a negative zeta potential (-24.23 ± 0.15 mV) in water. X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry data confirmed FMN present on the USPIO nanoparticle surface. FLUSPIO uptake in prostate cancer cells and human umbilical vein endothelial cells was significantly higher than that of control USPIO, while addition of excess of free FMN reduced accumulation. Similarly, in vivo MRI and histology showed specific FLUSPIO uptake by prostate cancer cells, tumor endothelial cells, and tumor-associated macrophages. Besides prominent tumor accumulation, FLUSPIO accumulated in the liver, spleen, lung, and skin. Hence, our data strengthen our hypothesis that targeting riboflavin receptors is an efficient approach to accumulate nanomedicines in tumors opening perspectives for the development of diagnostic and therapeutic systems.

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Section: Bio-distribution Of Fluspiosupporting

confidence: 60%

In vivo evaluation of riboflavin receptor targeted fluorescent USPIO in mice with prostate cancer xenografts

et al. 2016

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“…We could deliver much higher absorbed doses in the liver and spleen and beside that due Decay corrected data are presented as the mean values as the percentage of administered activity per gram (mean ± SD, n = 5) J Radioanal Nucl Chem to short blood half-life of 166 Ho-DTPA-SPIONs which was in accordance with our previous study [21,40].…”

Section: Discussionsupporting

confidence: 88%

“…In this study, the tracer was mostly accumulated in the liver and spleen, which were the major organs of accumulation throughout the study as shown in Table 1. This is in agreement with the reported literatures [21,40]. In 1988 Dr. Weissleder et al used the (AMI-25) which was SPIO with 80 nm mean size and they reported that after 1 h post i.v.…”

Section: Discussionsupporting

confidence: 87%

Biodistribution evaluation of 166Ho–DTPA–SPION in normal rats

Nosrati

Shanehsazzadeh

Yousefnia

et al. 2015

J Radioanal Nucl Chem

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Super paramagnetic iron oxide nanoparticles (SPION) with a cross-linked dextran coating can be used as a negative contrast agent in magnetic resonance imaging agent. This study describes the preparation, biodistribution of 166 Ho-DTPA-SPIONs after intravenous injection in normal Wistar rats. The biodistribution of 166 Ho-DTPASPIONs showed dramatic uptake in reticuloendothelial system (i.e., more than 60 and 15 % of injected dose was in liver and spleen at 30 min post-injection, respectively) and the clearance of the radiotracer is so fast in other organs. These results suggest that 166 Ho-DTPA-SPIONs could be good candidate for theranostic purposes in for liver and spleen malignancies.

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“…The microscopic size examination showed a change in the particles size post-centrifuge, and the biodistribution data reveal the efficiency and benefits of centrifuge in terms of preventing the uptake by liver and bone marrow [37,38].…”

Section: Discussionmentioning

confidence: 91%

“…The decay corrected acquisition protocol was set to 15 min per bed position. Figure 3 shows the distribution of 68 Ga-MAA in the first rat via PET/CT and PET maximum intensity projection (MIP) images [37]. In all of the acquisitions, the rats were placed in supine position and CT scans performed for anatomical reference and attenuation correction (spatial resolution 1.25 mm, 80 kV, 150 mAs) with a total CT scanning time of 20 s.…”

Section: Pet Scan Imagingmentioning

confidence: 99%