Biodiversity and phylogeny of novel Trichoderma isolates from mangrove sediments and potential of biocontrol against Fusarium strains

Abstract

Background: Studies carried out with novel 13 strains of Trichoderma, isolated from mangrove sediments (PE, Brazil) using morphophysiological and molecular characterization, followed evaluation of biocontrol using Fusarium strains isolated from Caatinga soil (PE, Brazil). Trichoderma strains were characterized by polyphasic taxonomic approach, and the extracted DNA was amplified with primers ITS 1 and 4, and sequenced. The biocontrol evaluation was conducted at 24 and 48 h of growth intervals by Tukey test, with a significance of 5%. Antibiosis tests were assessed in vitro by dual plate and partition plate techniques against Fusarium strains. Results: Trichoderma molecular identification, sequences of 500 bp were amplified, deposited into GenBank, and used for phylogenetic analyses. The strains were identified as T. asperellum (10), as T. harzianum (2) and one as T. longibrachiatum. Growth rate presented an average of 0.1207 cm h −1 for Trichoderma and lower growth rate of 0.031 cm h −1 for Fusarium spp., respectively. Antibiosis tests presented the best antagonist level of efficiency for T. asperellum UCP 0149 against F. solani UCP 1395 (82.2%) and F. solani UCP 1075 (70.0%), followed by T. asperellum UCP 0319 against F. solani UCP1083 (73.4%) and T. asperellum UCP 0168 against F. solani UCP1098 (71.5%), respectively. Conclusions: The data obtained in this study as tool for identification of novel Trichoderma strains serve as basis for development of several sustainable use for biotechnological processes. Those Trichoderma strains found promising for the management antagonistic potential and interaction could aid the conduct of biotechnological biocontrol of contaminants, and improve environmental conditions for the health of plants.

Coating seeds with Trichoderma strains promotes plant growth and enhance the systemic resistance against Fusarium crown rot in durum wheat

“…1). The mycelium, initially of a white color, acquired green, yellow shades, or remained white, due to the abundant production of conidia (Siddiquee, 2017 andFilizola et al 2019), which in consistent with the characteristics previously described for this fungus (El-Sobky et al 2019). The preliminary identification of these strains, based on colony morphology observations, was insufficient to reliably determine species.…”

“…The strain S.INAT displayed the most promising antagonistic potential to prevent the growth of F. culmorum, which deserve attention and further analysis of their ability to control disease development in controlled conditions as well as field experiments. The observed variations regarding the degree of antagonism among the different isolates against the same Fusarium strain may be indicative of the existence of specificity between the antagonist and the potential phytopathogen, suggesting the involvement of various genes and genetic factors interacting with the environment, and highlight the fact that more than one mechanism of action might be simultaneously involved in antagonistic actions (Filizola et al 2019).…”

“…The great economic and industrial interest in Trichoderma spp. has resulted in formulations of regulated and commercialized products for agricultural use (Filizola et al 2019). Seed coating with Trichoderma spp.…”

Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

“…A plug of B. cinerea was then placed equidistant from the other side of the plate and cultured at 26°C for 20 days. The distance of hyphal extension for each strain was measured (Dubey et al, 2014;Tzelepis et al, 2015;Filizola et al, 2019).…”

Control biológico de la pudrición de mazorca en genotipos de maíz con especies de Trichoderma

“…Éstos mecanismos indirectos y directos pueden actuar de manera coordinada y su importancia en el proceso de biocontrol depende de la cepa de Trichoderma, el hongo antagonizado, la especie de planta y las condiciones ambientales, incluida la disponibilidad de nutrientes, pH, temperatura, entre otros (Filizola et al, 2019). La activación de cada mecanismo implica la producción de compuestos y metabolitos específicos, como los factores de crecimiento de las plantas, enzimas hidrolíticas, sideróforos, antibióticos y nitrógeno (Rubio et al, 2014).…”