Biodiversity of organotin resistant Pseudomonas from west coast of India

Abstract

Five bacterial isolates were screened for resistance to organotin compound, i.e. tributyltin chloride (TBTC) up to 2 mM. The optimum pH, temperature and salinity for the growth of the isolates were found to be 7, 28 degrees C and 2.5%, respectively. The isolates were tested for survival tolerance to heavy metals (mercury, cadmium and zinc) and co-resistance to antibiotics viz. ampicillin, kanamycin, rifampicin, streptomycin, penicillin, chloramphenicol, tetracycline, nalidixic acid and neomycin. Although our earlier study reported that these five bacterial strains are of different species of Pseudomonas, our present 16S rRNA gene sequence analysis revealed that all the strains are Pseudomonas aeruginosa. One of five isolates P. aeruginosa strain 25W could grow in mineral salt medium with 2 mM of TBTC as a sole source of carbon and survive up to 5 mM of TBTC. In presence of 2 mM of TBTC there was comparable up-regulation of 45 kDa protein in the cell extract of the 25W isolate was found indicating involvement of certain enzymes in TBTC resistance.

YgiW homologous gene from Pseudomonas aeruginosa 25W is responsible for tributyltin resistance

“…In a previous study, Pseudomonas aeruginosa 25W was isolated and characterized (Roy et al, 2004). This isolate was found to possess a robust resistance not only to TBT, but also to heavy metals (Hg, Cd, Zn) (Roy and Nair, 2007). This report characterizes a novel TBT resistance gene, PA0320, of P. aeruginosa 25W.…”

“…It is recognized that many TBT-resistant bacteria show a cross-resistance to heavy metals (Pain and Cooney, 1998;Suzuki et al, 1992;Wuertz et al, 1991), and that P. aeruginosa 25W also exhibited such a high resistance (Roy and Nair, 2007). To learn whether Values indicate fold change (the mean of triplicate samples) with SD in gene expression of PA0320, compared with transcription level in the absence of TBT.…”

Supramolecular homobimetallic bis-diorganotin(IV) complexes of ditopic oxygen nitrogen donor ligand: synthesis, spectroscopic characterization, crystal structure and biological screening

“…The bacterial resistance to some Organotin(IV) compounds is reported in the literature. However, resistance mechanism is yet to be fully explored [44,45]. Compounds may exhibit bactericidal or bacteriostatic effects but the inhibitory action of organotin(IV) compounds may be due to their ability to inhibit ATP synthesis and can be justified through chelation theory.…”

Isolation and identification of potent tributyltin chloride utilizing Pseudomonas sp. strain VBAK from Vishakhapatnam shipping harbor sediments

“…M-l, was the first record of its kind [12]. Several reports have evidenced the studies on the isolation and characterization of TBT resistant bacteria from Indian coastal shore waters [13][14][15]; however, only a few reports revealed the detoxification of TBT mediated by microorganisms [1,12,14]. On the contrary, the reports are also meager on TBT-utilizing bacteria.…”