Biodiversity of rhizobia isolated from a wide range of forest legumes in Brazil

Moreira, F. M. S.; Haukka, Kaisa; Young, J. Peter W.

doi:10.1046/j.1365-294x.1998.00411.x

Cited by 108 publications

(79 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, in an analysis of a large collection of indigenous rhizobia in China, no B. elkanii strains were detected (Dr Wen-Xin Chen, personal communication). In conclusion, despite the low variability reported for the 16S rRNA region of Bradyrhizobium in some studies (So et al, 1994;Urtz & Elkan, 1996;Moreira et al, 1998;Molouba et al, 1999;van Berkum & Fuhrmann, 2000;Chen et al, 2000;Willems et al, 2001), and despite the coefficients used in some of the analyses being different, the strains from our study were joined at a very low level of similarity, 28 %, showing greater variability than any previous report. For example, an analysis of African indigenous soybean bradyrhizobia by RFLP-PCR with five restriction enzymes grouped the strains with a similarity of 70 % (Abaidoo et al, 2000), while in strains from native leguminous species of Senegal, the similarity with five restriction enzymes was approximately 74 %, decreasing to 55 % when a strain from Aeschynomene was included (Doignon-Bourcier et al, 1999).…”

Section: Discussioncontrasting

confidence: 83%

“…Partial or complete 16S rRNA gene sequences, which have also been used extensively for studying the phylogeny of rhizobia (e.g. Young et al, 1991;Oyaizu et al, 1992;Yanagi & Yamasato, 1993;van Rossum et al, 1995;Urtz & Elkan, 1996;Moreira et al, 1998;Vinuesa et al, 1998;Wang et al, 1999;Chen et al, 2000; JaraboLorenzo et al, 2000), have contributed to the recent descriptions of four new genera and several rhizobial species. However, there are reports showing that, despite a high level of diversity in morphological, physiological and genetic properties, diversity is low in the 16S rRNA gene sequences of strains of Bradyrhizobium investigated so far (So et al, 1994, Urtz & Elkan, 1996Molouba et al, 1999;Vinuesa et al, 1998;Chen et al, 2000;van Berkum & Fuhrmann, 2000;Willems et al, 2001;Qian et al, 2003).…”

mentioning

confidence: 99%

See 1 more Smart Citation

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

Germano

Menna

Mostasso

et al. 2006

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

Genetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N 2 -fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76 T ; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined. INTRODUCTIONThe Leguminosae (known as the Fabaceae in the USA) is one of the largest families of plants, with over 18 000 species classified into around 650 genera, representing approximately one-twelfth of all known flowering plants and occupying nearly all terrestrial biomes (Polhill & Raven, 1981). Many species within this family are capable of establishing symbioses with a group of bacteria collectively called rhizobia, of which the most important feature is the capacity...

show abstract

Section: Discussioncontrasting

confidence: 83%

mentioning

confidence: 99%

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

Germano

Menna

Mostasso

et al. 2006

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

show abstract

“…The capacity to nodulate appears to be the rule for leguminous plants within the AR, since more than 80 % of species of the subfamilies Papilionoideae and Mimosoideae do it; however, only about 20 % of the Caesalpinioideae are capable of forming nodules (de Faria, et al, 1989(de Faria, et al, , 2010Moreira, et al, 1993). Legumes contribute significantly to the AR N-balance, with more than 1006Amazonian legumes; however, the diversity of the nodule bacteria remains poorly understood (Moreira et al, 1998). Recent studies, using soil-trapping experiments with promiscuous legumes, including Macroptilium atropurpureum and Vigna unguiculata, have shown that AR soils contain diverse rhizobia (Jesus et al, 2005;Chagas Junior et al, 2010;Guimarães et al, 2012;Silva et al, 2012).…”

mentioning

confidence: 99%

Bradyrhizobium manausense sp. nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils

Silva

Meyer

Simões‐Araújo³

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Root nodule bacteria were trapped within cowpea (Vigna unguiculata) in soils with different cultivation histories collected from the Amazonian rainforest in northern Brazil. Analysis of the 16S rRNA gene sequences of six strains (BR 3351 T , BR 3307, BR 3310, BR 3315, BR 3323 BR and BR 3361) isolated from cowpea nodules showed that they formed a distinct group within the genus Bradyrhizobium, which was separate from previously identified type strains. Phylogenetic analyses of three housekeeping genes (glnII, recA and rpoB) revealed that Bradyrhizobium huanghuaihaiense CCBAU 23303 T was the most closely related type strain (96 % sequence similarity or lower). Chemotaxonomic data, including fatty acid profiles (predominant fatty acids being C 16 : 0 and summed feature 8), the slow growth rate and carbon compound utilization patterns supported the assignment of the strains to the genus Bradyrhizobium. The results of DNA-DNA hybridizations, antibiotic resistance and physiological tests differentiated these novel strains from the most closely related species of the genus Bradyrhizobium with validly published names. Symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) grouped the novel strains of the genus Bradyrhizobium together with Bradyrhizobium iriomotense strain EK05 T , with 94 % and 96 % sequence similarity, respectively. Based on these data, these six strains represent a novel species for which the name Brabyrhizobium manausense sp. nov. (BR 3351 T 5HAMBI 3596 T ), is proposed.The family Leguminosae is one of the most abundant plant families in the Amazonian rainforest (AR) with more than 1240 species and 140 genera of all three subfamilies (Caesalpinioideae, Mimosoideae and Papilionoideae) catalogued (Ducke, 1949;Silva et al., 1988). This family is ecologically and economically important to the biome, because it contains pioneer species that revegetate bare soils, species that produce grain for food and species used for timber production. Many legume species are capable of forming root (or sometimes stem) nodules, which contain nitrogen-fixing bacterial symbionts, collectively known as 'rhizobia ' (Sprent, 1995). The capacity to nodulate appears to be the rule for leguminous plants within the AR, since more than 80 % of species of the subfamilies Papilionoideae and Mimosoideae do it; however, only about 20 % of the Caesalpinioideae are capable of forming nodules (de Faria, et al., 1989(de Faria, et al., , 2010Moreira, et al., 1993). Legumes contribute significantly to the AR N-balance, with more than 1006 10 8 kg of nitrogen being fixed annually (Salati et al., 1982;Martinelli et al., 1992;Gehring, et al., 2005).Bacteria of the genus Bradyrhizobium have been reported to comprise the majority of root nodule bacteria isolated from The GenBank/EMBL/DDBJ accession number for the 16S rRNA, glnII, recA, rpoB, dnaK, gyrB, nodC and nifH gene sequences of BR3351 T are HQ641226, KF785986, KF785992, KF785998, KF786000, KF786001, KF786002 and KF786003, respectively. Abbreviation: AR, Amazonian ra...

show abstract

“…A estirpe Br 4406, classificada como B. japonicum (Moreira et al, 1998), microssimbionte de Enterolobium contortisilyquum. (Vell) Morong (tamboril), vem-se destacando principalmente, pela tolerância à acidez (Ribeiro Jr. et al, 1986) e a metais pesados (Matsuda, 2000;Trannin et al, 2001).…”

Section: Estirpes Estudadasunclassified

Influência do pH do meio de cultivo e da turfa no comportamento de estirpes de Bradyrhizobium

Miguel

Moreira²

2001

Rev. Bras. Ciênc. Solo

Self Cite

View full text Add to dashboard Cite

RESUMOA acidez dos solos representa um problema que afeta grandes áreas agrícolas pelo mundo, principalmente nos trópicos, onde fósforo e nitrogênio também são limitantes. No caso do nitrogênio, a fixação biológica torna-se uma das alternativas mais viáveis do ponto de vista ecológico e econômico, por diminuir o uso e o impacto causado pelos fertilizantes nitrogenados. Neste trabalho, foram realizados dois experimentos in vitro e um em casa de vegetação com quatro estirpes de Bradyrhizobium (Br 4406, Br 29, SEMIA 587 e INPA 03-11B), no Departamento de Ciência do Solo (UFLA), de julho de 1998 a julho de 1999, para verificar o efeito de três valores de pH (5,0; 6,0; 6,9) no crescimento destas em meio de cultura YM, na sua simbiose com soja, assim como na sua sobrevivência em inoculantes produzidos com turfa. No primeiro experimento, as estirpes de Bradyrhizobium tiveram um comportamento diferenciado em meio líquido, obtendo melhor desempenho em pH 6,0, tanto em número de unidades formadoras de colônias quanto em produção de exopolissacarídeos. +No segundo experimento, o número de nódulos, a atividade da nitrogenase (Nase), as massas secas de nódulos, raízes e parte aérea de plantas de soja, de modo geral, não foram influenciados pelos valores de pH de cultivo das estirpes no inoculante. Entretanto, a estirpe INPA 03-11B mostrou-se efetiva, apresentando número de nódulos e atividade de Nase semelhantes aos de Br 29 e SEMIA 587, que são recomendadas como estirpes inoculantes, devendo, assim, ser indicada para testes de eficiência em campo. No terceiro experimento, com exceção da Br 29, que atingiu maior sobrevivência de células em pH 6,0, as outras estirpes tiveram sobrevivência semelhante neste valor de pH e em pH 6,9. O melhor desempenho das estirpes de Bradyrhizobium em pH 6,0 no meio de cultura e em turfa demonstrou a possibilidade do uso de inoculantes corrigidos para esse valor de pH, como modo de pré-adaptação às condições de acidez dos solos tropicais.Termos de indexação: fixação biológica do N 2 , soja, inoculante turfoso, tolerância à acidez, produção de exopolissacarídeos.

show abstract

Biodiversity of rhizobia isolated from a wide range of forest legumes in Brazil

Cited by 108 publications

References 37 publications

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

Bradyrhizobium manausense sp. nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils

Influência do pH do meio de cultivo e da turfa no comportamento de estirpes de Bradyrhizobium

Contact Info

Product

Resources

About