Bioelectrochemical Analysis of Neuropathy Target Esterase Activity in Blood

Sigolaeva, Larisa V.; Makower, Alexander; Eremenko, A. V.; Махаева, Галина Ф.; Malygin, V. V.; Kurochkin, Ilya N.; Scheller, Frieder W.

doi:10.1006/abio.2000.4822

Cited by 27 publications

(36 citation statements)

References 27 publications

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“…Thus, if the need were to arise to assess exposures of individuals to neuropathic OP compounds, it would be advantageous to be able to assay NTE in small volumes of whole blood (Sigolaeva et al, 2001). …”

Section: And Between Brain and Blood (R = 997) The Results Suggest mentioning

confidence: 99%

“…The absorbance maximum of the red phenol chromophore overlaps substantially with that of whole blood homogenates, and dilution of the blood to remove the interfering absorbance decreases NTE activity below the detection limit of the colorimetric assay (Sigolaeva et al, 2001). Thus, the colorimetric assay cannot be used to assay NTE in whole blood.…”

Section: And Between Brain and Blood (R = 997) The Results Suggest mentioning

confidence: 99%

“…Data showed that the tyrosinase carbon-paste electrode improved the sensitivity of the NTE assay 10-fold compared to the colorimetric method or an earlier amperometric technique. Moreover, the new electrode operates in a flow-injection mode that requires only 2 to 3 min per analysis (Sigolaeva et al, 1999(Sigolaeva et al, , 2000(Sigolaeva et al, , 2001.…”

Section: And Between Brain and Blood (R = 997) The Results Suggest mentioning

confidence: 99%

“…The tyrosinase electrode was prepared as described previously (Sigolaeva et al, 2001). All measurements were performed with an applied potential of −150 mV versus Ag/AgCl.…”

Section: Tyrosinase Carbon-paste Electrode and Biosensormentioning

confidence: 99%

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Biosensor Detection of Neuropathy Target Esterase in Whole Blood as a Biomarker of Exposure to Neuropathic Organophosphorus Compounds

Махаева¹,

Sigolaeva²,

Zhuravleva³

et al. 2003

Journal of Toxicology and Environmental Health Part A

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Public Reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. 44379.1-LSNeuropathy target esterase (NTE) is the target for neuropathic organophosphorus compounds (OPs) that produce delayed neurotoxicity (OPIDN). Inhibition/aging of brain NTE predicts the potential for OPIDN in animal models. Lymphocyte NTE has also found use as a biomarker of human exposure to neuropathic OPs. Recently, a sensitive NTE biosensor was developed using a tyrosinase carbon-paste electrode for amperometric (Amp) detection of phenol produced by hydrolysis of the substrate, phenyl valerate. The I50 (20 min at 37 °C) for N,N'-di-2 propylphosphorodiamidofluoridate (mipafox) against hen lymphocyte NTE was 6.94 ± 0.28 •uM (Amp) and 6.02 ± 0.71 uM colorimetrically (Col). For O,O-di-1-propyl O-2,2-dichlorvinyl phosphate (PrDChVP), the I50 against hen brain NTE was 39 ± 8 nM (Amp) and 42 ± 2 nM (Col). I50 values (Amp) for PrDChVP against hen and human blood NTE were 66 ± 3 and 70 ± 14 nM, respectively. NTE activities in brain, lymphocytes, and blood were measured 24 h after dosing hens with PrDChVP. NTE inhibition was highly correlated between brain and lymphocyte (r = 0.994) and brain and blood (r = 0.997). Biosensor NTE assay for whole blood could serve as a biomarker of exposure to neuropathic OPs.12 (excluding cover forms) Biosensor, Delayed neurotoxicity, Mipafox, Organophosphorus compounds (OPs), Neuropathy target esterase (NTE).

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Section: And Between Brain and Blood (R = 997) The Results Suggest mentioning

confidence: 99%

Section: And Between Brain and Blood (R = 997) The Results Suggest mentioning

confidence: 99%

Section: And Between Brain and Blood (R = 997) The Results Suggest mentioning

confidence: 99%

“…The tyrosinase electrode was prepared as described previously (Sigolaeva et al, 2001). All measurements were performed with an applied potential of −150 mV versus Ag/AgCl.…”

Section: Tyrosinase Carbon-paste Electrode and Biosensormentioning

confidence: 99%

See 2 more Smart Citations

Biosensor Detection of Neuropathy Target Esterase in Whole Blood as a Biomarker of Exposure to Neuropathic Organophosphorus Compounds

Махаева¹,

Sigolaeva²,

Zhuravleva³

et al. 2003

Journal of Toxicology and Environmental Health Part A

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show abstract

“…In the first step, a solution containing phenyl valerate is brought into contact with NEST or NTE protein solution, whose esterase activity reacts with a portion of the artificial substrate phenyl valerate to form phenol. In the second step, the concentration of phenol in the solution is determined either colorimetrically, in the presence of 4-amino antipyrine (Kayyali et al, 1991), or electrochemically, in the presence of tyrosinase enzyme (Sigolaeva et al, 2001;Sokolovskaya et al, 2005). Tyrosinase converts phenol first to catechol and then to o-quinone, which can be measured electrochemically at an electrode (Makhaeva et al, 2003).…”

Section: Introductionmentioning

confidence: 99%