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Public Reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. 44379.1-LSNeuropathy target esterase (NTE) is the target for neuropathic organophosphorus compounds (OPs) that produce delayed neurotoxicity (OPIDN). Inhibition/aging of brain NTE predicts the potential for OPIDN in animal models. Lymphocyte NTE has also found use as a biomarker of human exposure to neuropathic OPs. Recently, a sensitive NTE biosensor was developed using a tyrosinase carbon-paste electrode for amperometric (Amp) detection of phenol produced by hydrolysis of the substrate, phenyl valerate. The I50 (20 min at 37 °C) for N,N'-di-2 propylphosphorodiamidofluoridate (mipafox) against hen lymphocyte NTE was 6.94 ± 0.28 •uM (Amp) and 6.02 ± 0.71 uM colorimetrically (Col). For O,O-di-1-propyl O-2,2-dichlorvinyl phosphate (PrDChVP), the I50 against hen brain NTE was 39 ± 8 nM (Amp) and 42 ± 2 nM (Col). I50 values (Amp) for PrDChVP against hen and human blood NTE were 66 ± 3 and 70 ± 14 nM, respectively. NTE activities in brain, lymphocytes, and blood were measured 24 h after dosing hens with PrDChVP. NTE inhibition was highly correlated between brain and lymphocyte (r = 0.994) and brain and blood (r = 0.997). Biosensor NTE assay for whole blood could serve as a biomarker of exposure to neuropathic OPs.12 (excluding cover forms) Biosensor, Delayed neurotoxicity, Mipafox, Organophosphorus compounds (OPs), Neuropathy target esterase (NTE).