Bioencapsulation of Somatic Embryos in Woody Plants

Bapat, V. A.; Mhatre, Minal

doi:10.1007/1-4020-2985-3_42

Cited by 20 publications

(7 citation statements)

References 19 publications

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“…Currently used broader definition of artificial seed is 'an artificially encapsulated somatic embryo, shoot or any other meristematic tissue which can develop into a plant under in vitro or in vivo conditions' (Bapat and Mhatre 2005). Artificial seed production is a potential technique for plant multiplication and preservation, especially as it has been considered to be promising for propagation of no-seed producing plants, transgenic plants and other plants that need to keep superior traits by means of asexual propagation (Saiprasad 2001).…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Islam

Bari

2014

J bio-sci.

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Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion:The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations

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Section: Introductionmentioning

confidence: 99%

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Islam

Bari

2014

J bio-sci.

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“…Automation of synthetic seed production is the final goal of commercial seed industries. The combination of a number of factors including advanced tissue culture technology, bioreactors, somatic embryo encapsulation, and development of appropriate synthetic seed coating material requires further study to meet the goal of producing millions of synthetic seeds in a short time and cutting the cost of seed production ( Ara et al, 2000 ; Bapat and Mhatre, 2005 ; Sharma et al, 2013 ; Gantait et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…These agents include sodium alginate, potassium alginate, sodium pectate, and carrageenan among others, with sodium alginate being the most common ( Malabadi and Staden, 2005 ; Pintos et al, 2008 ; Gantait et al, 2015 ). Carbon sources, plant growth regulators, and antimicrobial agents were also added to the hydrogel to facilitate growth and increase the survival rate of the encapsulated propagules ( Bapat and Mhatre, 2005 ; Rai et al, 2008b ). Finally, the encapsulated propagules were cultured on media or in the field for plantlet conversion.…”

Section: Application Of Somatic Embryogenesis In Woody Plantsmentioning

confidence: 99%

Application of Somatic Embryogenesis in Woody Plants.

et al. 2016

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Somatic embryogenesis is a developmental process where a plant somatic cell can dedifferentiate to a totipotent embryonic stem cell that has the ability to give rise to an embryo under appropriate conditions. This new embryo can further develop into a whole plant. In woody plants, somatic embryogenesis plays a critical role in clonal propagation and is a powerful tool for synthetic seed production, germplasm conservation, and cryopreservation. A key step in somatic embryogenesis is the transition of cell fate from a somatic cell to embryo cell. Although somatic embryogenesis has already been widely used in a number of woody species, propagating adult woody plants remains difficult. In this review, we focus on molecular mechanisms of somatic embryogenesis and its practical applications in economic woody plants. Furthermore, we propose a strategy to improve the process of somatic embryogenesis using molecular means.

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“…Year-around production, ease of handling, and direct delivery of genetically uniform germplasm to fi eld or greenhouse are the main advantages of encapsulation technology. The direct delivery of encapsulated material will save many subcultures to obtain plantlets (Maruyama et al 1997 ;Bapat and Mhatre 2005 ). Rajkumar and Rajanna ( 2011 ) provide d a report on ex situ conservation of climbing plants at the University of Agricultural Sciences, Bangalore, Karnataka.…”

Section: Synseed Technologymentioning

confidence: 98%

Biodiversity Conservation with Special Reference to Medicinal Climbers: Present Scenario, Challenges, Strategies, and Policies

Sharma

Arya

2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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Climbing plants typically contribute 2-15 % of the leaf biomass and about 5 % of the wood biomass to forests. In India, they are widely distributed from Nilgiris to Himalayas to Andaman Nicobar Islands. Phylogenetically, climbers are found in over 125 families of fl owering plants as well as among several fern groups and even in one signifi cant gymnosperm genus, Gnetum . They are largely used by all divisions of the population either directly as folk medications or indirectly in the preparation of recent pharmaceuticals. Climbers also play a vital role in horticulture and agriculture. Thus, people depend on climbers for several purposes like for medications, non-timber forest products, food, etc. Nowadays, plant-based pharmaceutical industries have been increased due to various advantages of plant-based medicine over the synthetic ones. Wild plants are the good source to provide the raw materials for such industries. Unfortunately, a huge number of medicinal climbers have been listed in Red Data Book and are on the verge of extinction due to unsustainable collection of plants, habitat loss, climate change, and industrialization coupled with urbanization. Thus, biodiversity conservation is an utmost concern globally. Various strategies are in use for biodiversity conservation such as in situ strategy, ex situ strategy, reduction of anthropogenic pressures, and rehabilitation of threatened species. Protection and preservation of germplasm within national parks, biosphere reserves, and sanctuaries are the examples of in situ conservation. However, it is not an effective means of conservation. In this regard, biotechnological approach like plant tissue culture technique proved to be fruitful. Plant tissue culture can be applied to the rapid propagation and ex situ conservation of rare, endemic, and endangered medicinal plants as explained by several authorities.

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Bioencapsulation of Somatic Embryos in Woody Plants

Cited by 20 publications

References 19 publications

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Application of Somatic Embryogenesis in Woody Plants.

Biodiversity Conservation with Special Reference to Medicinal Climbers: Present Scenario, Challenges, Strategies, and Policies

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