Bioengineered protein-based nanocage for drug delivery

Lee, Eun Jung; Lee, Na Kyeong; Kim, In San

doi:10.1016/j.addr.2016.03.002

Cited by 191 publications

(156 citation statements)

References 238 publications

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“…DOX loading via non-covalent or covalent attachments on various icosahedral (sphere-like) VNPs has been reported (reviewed in 52 ). For example, DOX has been covalently attached to 30 nm-sized CPMV (up to 270 DOX per VNP) or Qβ bacteriophage (up to 500 DOX per VNP).…”

Section: Discussionmentioning

confidence: 99%

Potato virus X, a filamentous plant viral nanoparticle for doxorubicin delivery in cancer therapy

et al. 2017

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Plant viral nanoparticles (VNPs) are a novel class of nanocarriers with implications for drug delivery in cancer therapy. VNPs are characterized by their highly symmetrical nanoscale structures. Furthermore, plant VNPs are biocompatible, biodegradable, and non-infectious in mammals. VNPs provide a proteinaceous platform technology that can be readily engineered to carry contrast agents and therapies using chemical and genetic modifications. Of particular interest are high aspect ratio, elongated filaments such as the ones formed by potato virus X (PVX, measuring 515 × 13 nm). PVX has demonstrated enhanced tumor homing and penetration properties compared to spherical counterparts. Here, we sought to investigate the potential of PVX as a drug carrier delivering doxorubicin (DOX), a commonly used cancer chemotherapy. We synthesized therapeutic PVX nanoparticles using a simple in-solution mixing protocol; after 5 days of mixing of DOX and PVX and ultra-centrifugal purification, ~1000 DOX per PVX were stably associated with the carrier, most likely based on hydrophobic interaction. Efficacy and drug activity of PVX-DOX were confirmed using a panel of cancer cell lines including ovarian cancer, breast cancer, and cervical cancer. Lastly, we demonstrated treatment of athymic mice bearing human MDA-MB-231 breast cancer xenografts: PVX-DOX treatment resulted in reduced tumor growth in this model. Our results open the door for further development of PVX and other high aspect ratio plant VNPs for applications in cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Potato virus X, a filamentous plant viral nanoparticle for doxorubicin delivery in cancer therapy

et al. 2017

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“…Natural structure-based nanocages (such as the ferritins, small heat shock proteins, and vaults) and most of the viruses (virus-like particles, cowpea chlorotic mottle virus, cowpea mosaic virus, bacteriophage, and drug loadings in protein-based nanocages) are all important examples. 103 A safe and smart pH-sensitive nanocompound drug delivery with high drug delivery ability was prepared by conjugating DOX to xyloglucan (XG) using acid-cleavable hydrazone bonds and, then, encapsulating DOX by the self-assembly of XG-DOX conjugates to increase drug delivery into the tumor cells. 104 The toxicity effects, such as the risk of bleeding due to heparin's anticoagulant activity, decreased using a novel combination nanosystem of low molecular weight heparin and ursolic acid (UA), which is also an angiogenesis inhibitor for tumor treatment.…”

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confidence: 99%

A review of drug delivery systems based on nanotechnology and green chemistry: green nanomedicine

Jahangirian

Lemraski

Webster

et al. 2017

IJN

449

216

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This review discusses the impact of green and environmentally safe chemistry on the field of nanotechnology-driven drug delivery in a new field termed “green nanomedicine”. Studies have shown that among many examples of green nanotechnology-driven drug delivery systems, those receiving the greatest amount of attention include nanometal particles, polymers, and biological materials. Furthermore, green nanodrug delivery systems based on environmentally safe chemical reactions or using natural biomaterials (such as plant extracts and microorganisms) are now producing innovative materials revolutionizing the field. In this review, the use of green chemistry design, synthesis, and application principles and eco-friendly synthesis techniques with low side effects are discussed. The review ends with a description of key future efforts that must ensue for this field to continue to grow.

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“…Hollow particles with sizes in the nanometer range (nanocages) have found widespread application in the medical field. For instance, the encapsulation of pharmaceutical drugs inside nanocages has tremendously increased the control over drug delivery on the molecular level . By modifying the exterior of the particles, targeting towards a desired tissue can be achieved, which reduces the side effects of the drug.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the encapsulation of pharmaceutical drugs inside nanocages has tremendously increased the control over drug delivery on the molecular level. [1,2] By modifying the exterior of the particles, targeting towards ad esired tissue can be achieved, which reduces the side effects of the drug.A tt he same time, the nanocage enhances the stabilityo ft he drug and increasesits half-life in vivo by shieldingi tf rom interfering external influences. The success of these types of designsisi llustrated by liposomal drug-delivery systems thata re now on the market or in clinical trials.…”

Section: Introductionmentioning

confidence: 99%

Self‐Assembly and Stabilization of Hybrid Cowpea Chlorotic Mottle Virus Particles under Nearly Physiological Conditions

Timmermans

Vervoort

Schoonen

et al. 2018

Chemistry An Asian Journal

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Capsids of the cowpea chlorotic mottle virus (CCMV) hold great promise for use as nanocarriers in vivo. A major drawback, however, is the lack of stability of the empty wild-type virus particles under physiological conditions. Herein, the assembly behavior and stability under nearly physiological conditions of protein-based block copolymers composed of the CCMV capsid protein and two hydrophobic elastin-like polypeptides are reported. UV/Vis spectroscopy studies, dynamic light-scattering analysis, and TEM measurements demonstrate that both hybrid variants form stable capsids at pH 7.5, physiological NaCl concentration, and 37 °C. The more hydrophobic variant also remains stable in a cell culture medium. These engineered, hybrid CCMV capsid particles can therefore be regarded as suitable candidates for in vivo applications.

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Bioengineered protein-based nanocage for drug delivery

Cited by 191 publications

References 238 publications

Potato virus X, a filamentous plant viral nanoparticle for doxorubicin delivery in cancer therapy

Potato virus X, a filamentous plant viral nanoparticle for doxorubicin delivery in cancer therapy

A review of drug delivery systems based on nanotechnology and green chemistry: green nanomedicine

Self‐Assembly and Stabilization of Hybrid Cowpea Chlorotic Mottle Virus Particles under Nearly Physiological Conditions

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