In around the world, mosquito control is considered a most important because of the incapable of synthetic insecticides and the ecological pollution about by them. In this manner, need the eco-friendly insecticides to efficient control the mosquito disease is the need of the hour. We synthesized the eco-friendly of zinc oxide nanoparticles (ZnO-NPs) using the
Knoxia sumatrensis
aqueous leaf extract (
Ks-
ALE) as a reducing and stabilizing agent. The synthesis of ZnO-NPs was confirmed by UV with an absorption peak at 354 nm. ZnO-NPs crystal structure was analyzed by X-ray diffraction (XRD). Fourier transform infrared spectroscopy (FT-IR) spectra revealed the chloride, cyclic alcohols, sulfonamies, carboxylic acids, oximes, phosphines, alkenes and alcohol & phenol. Field emission-scanning electron microscopy (FE-SEM) showed that the NP’s are rod shaped with 50–80 nm size and also energy dispersive spectra (EDaX) spectra showed presence of zinc. Antioxidant assay showed superior activity and evidenced by DPPH, ABTS and H
2
O
2
radical assays. Furthermore, the ZnO-NPs exhibited strong activity in MCF-7 cell line with IC
50
value is 58.87 μg/mL. Mosquito larvicidal activity of ZnO-NPs produced significant activity and excellent larvicidal activity was noticed in
Cx. quinquefasciatus
with LC
50
0.08, mg/mL and LC
90
19.46 mg/mL. This study suggests that synthesized ZnO-NPs using
Knoxia sumatrensis
leaf extract have good biological activities and it makes them an ideal candidate for pharmacological studies.