Bioengineered skin constructs

Falanga, Vincent

doi:10.1016/b978-0-12-818422-6.00073-3

Cited by 8 publications

(7 citation statements)

References 94 publications

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“…At the same time, inflammatory cytokines that control blood flow to the wound bed are released, and lymphocytes and macrophages are recruited to fight infection. Subsequent to the primary inflammatory response, the proliferation phase takes place with angiogenesis, fibroblast proliferation, and collagen deposition [ 4 ]. Later, granulation tissue, a tissue with a high content of cells and blood capillaries, is formed.…”

Section: Introductionmentioning

confidence: 99%

“…Subsequently, these abnormalities may be associated with a loss of tissue viability, suboptimal local tissue permeability, and an elevated and sustained inflammatory response [ 4 ]. One of the key differences between acute and chronic wounds is that the ordered phases associated with acute wound healing are not identical to the asynchrony of the healing process occurring in chronic human wounds [ 5 ]. Within the chronic wound, several phases of the wound repair process may be occurring simultaneously or out of sequence [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…One of the key differences between acute and chronic wounds is that the ordered phases associated with acute wound healing are not identical to the asynchrony of the healing process occurring in chronic human wounds [ 5 ]. Within the chronic wound, several phases of the wound repair process may be occurring simultaneously or out of sequence [ 5 ]. Nonhealing wounds are plagued by bacterial and, in rare circumstances, fungal colonization or infection [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Within the chronic wound, several phases of the wound repair process may be occurring simultaneously or out of sequence [ 5 ]. Nonhealing wounds are plagued by bacterial and, in rare circumstances, fungal colonization or infection [ 5 ]. These features include a lack of epithelium and its barrier capabilities, continuous wound exudation caused by bacterial products and inflammation, inadequate blood flow, and hypoxia [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Structural and biological engineering of 3D hydrogels for wound healing

Norahan

Pedroza-González

Sánchez-Salazar

et al. 2023

Bioactive Materials

118

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Structural and biological engineering of 3D hydrogels for wound healing

Norahan

Pedroza-González

Sánchez-Salazar

et al. 2023

Bioactive Materials

118

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“…Re-epithelialization is characterized by forming a new epithelial layer and cellular migration. The epithelial barrier between the wound and the environment prevents tissue water loss and pathogen contamination. , Figure d demonstrates an increase in re-epithelialization at day 6 post-nanofiber treatment. The minimum rate of re-epithelialization was seen in the control group, while the peptide-loaded group exhibited the highest re-epithelialization rate after 6 days (Figure f,d).…”

Section: Resultsmentioning

confidence: 99%

Cross-Linked Electrospun pH-Sensitive Nanofibers Adsorbed with Temporin-Ra for Promoting Wound Healing

Koohzad

Asoodeh

2023

ACS Appl. Mater. Interfaces

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Bioresponsive nanodrug delivery systems have excellent potential in tissue engineering applications. Poly-anionic and poly-cationic biopolymers have provided a superior platform for designing pH-sensitive drug delivery systems. In this regard, hyaluronic acid–chitosan–polyvinyl alcohol complex nanofibers with high quality and reproducibility were produced by optimizing the solution preparation process. In addition, the synthesized composite nanofiber, with 66.82 kN/mm toughness, 200% swelling ratio, and 60% porosity, exhibited excellent properties to meet the requirements of the ideal wound dressing. Green cross-linking with citric acid prevented the destruction of the nanofiber even after prolonged immersion in biological solutions. ζ potential studies demonstrated that the synthesized nanofiber has a negative surface charge (∼−30) at physiological pH. The pK a of the temporin-Ra peptide is about 10, and as a result the peptide molecules have a net positive charge in physiological conditions. Therefore, peptide molecules immobilized on the synthesized scaffold based on surface adsorption. In vivo evaluation has proven that the wound bed has an alkaline environment, facilitating peptide release from the nanofiber scaffold. Electrospun nanofibers can imitate the architecture of the extracellular matrix for accelerating wound healing. In vitro investigation showed better adhesion, proliferation, migration, and fibroblast cell growth on peptide-loaded nanofiber samples than other groups. In vivo studies on full-thickness wounds in the mouse model indicated that the designed nanofiber was gradually absorbed without causing dryness or infection. On day 6, the peptide-loaded nanofiber revealed 60% wound closure compared to the control group (17%). In addition, based on histological studies, the composite nanofiber demonstrated excellent tissue repair ability, hence these active nanofiber mats can be a good alternative to existing wound dressings. Gene expression studies show that the antimicrobial peptide promotes the inflammatory phase of wound healing in a shorter time frame by accelerating the tumor necrosis factor-α cytokine response.

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