Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring

Zubkovs, Vitālijs; Wang, Hanxuan; Schuergers, Nils; Weninger, Astrid; Glieder, Anton; Cattaneo, Stefano; Boghossian, Ardemis A.

doi:10.1039/d2na00092j

Cited by 12 publications

(10 citation statements)

References 57 publications

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“…The authors suggested direct reversible doping of nanotube surface defects, mediated by molecularly adsorbed oxygen, as a mechanism for the observed optical modulation. A subsequent study from the same group used bioconjugation reactions to outfit GOx with pyrene moieties that can anchor the enzyme to the nanotube surface through π-stacking interactions . Several recombinant GOx variants were anchored on nanotube surfaces and screened for their optical responses to glucose, which led to the identification of a variant that produced optical modulations with single milli-molar sensitivity, a feat the authors attribute to the oriented enzyme loading facilitated by pyrene binding.…”

Section: Cnm Fluorescent Probe Applicationsmentioning

confidence: 99%

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

Krasley,

Li,

Galeana

et al. 2024

Chem. Rev.

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Fluorescent carbon nanomaterials have broadly useful chemical and photophysical attributes that are conducive to applications in biology. In this review, we focus on materials whose photophysics allow for the use of these materials in biomedical and environmental applications, with emphasis on imaging, biosensing, and cargo delivery. The review focuses primarily on graphitic carbon nanomaterials including graphene and its derivatives, carbon nanotubes, as well as carbon dots and carbon nanohoops. Recent advances in and future prospects of these fields are discussed at depth, and where appropriate, references to reviews pertaining to older literature are provided.

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Section: Cnm Fluorescent Probe Applicationsmentioning

confidence: 99%

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

Krasley,

Li,

Galeana

et al. 2024

Chem. Rev.

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“…Enzymatic glucose sensors have garnered significant attentions due to their selectivity, simplicity, and sensitivity. In recent studies, novel metal nanoclusters have been developed for the detection of the oxidation product of glucose by the glucose oxidase enzyme (GOx) [ 67 , 68 ]. Bovine serum albumin stabilized Au nanoclusters (BSA-AuNCs) and 1,2-bis [4-(3-sulfopropoxy)phenyl]-1,2-diphenylethylene (BSPOTPE) sodium were employed as the fluorescence detection probe and reference probe, respectively [ 69 ].…”

Section: Enzyme-based Sensing Platformmentioning

confidence: 99%

Sensitive and specific detection of saccharide species based on fluorescence: update from 2016

Huang

Han

et al. 2023

Anal Bioanal Chem

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Increasing evidence supports the critical role of saccharides in various pathophysiological steps of tumor progression, where they regulate tumor proliferation, invasion, hematogenic metastasis, and angiogenesis. The identification and recognition of these saccharides provide a solid foundation for the development of targeted drug preparations, which are however not fully understood due to their complex and similar structures. In order to achieve fluorescence sensing of saccharides, extensive research has been conducted to design molecular probes and nanoparticles made of different materials. This paper aims to provide in-depth discussion of three main topics that cover the current status of the carbohydrate sensing based on the fluorescence sensing mechanism, including a phenylboronic acid-based sensing platform, non-boronic acid entities, as well as an enzyme-based sensing platform. It also highlights efforts made to understand the recognition mechanisms and improve the sensing properties of these systems. Finally, we present the challenge of achieving high selectivity and sensitivity recognition of saccharides, and suggest possible future avenues for exploration. Graphical Abstract

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“…[14] Taking advantage of these attractive properties of SWCNTs and specifically the glucose recognizability of GOx, several SWCNT-based glucose nanosensors have been reported, with challenges including low synthesis scalability, sub-par colloidal stability, and poor reversibility. [7,[15][16][17][18] The synthesis of SWCNT-based nanosensors via biomolecule-SWCNT conjugation is typically accomplished through ultrasonication of polymer-SWCNT conjugates or through dialysis-based ligand exchange of biomolecule-SWCNT conjugates. [5,19] While probe tip sonication is quick and high-yielding, this method is typically deemed unsuitable for protein-SWCNT conjugation due to the denaturation of proteins under the high shear stresses induced by probe tip sonication.…”

Section: Introductionmentioning

confidence: 99%

“…Owing to their substrate‐specific enzymatic activity, enzymes can be used to rationally design nanosensors for a specific analyte of interest [14] . Taking advantage of these attractive properties of SWCNTs and specifically the glucose recognizability of GOx, several SWCNT‐based glucose nanosensors have been reported, with challenges including low synthesis scalability, sub‐par colloidal stability, and poor reversibility [7,15–18] …”

Section: Introductionmentioning

confidence: 99%

Engineered Glucose Oxidase‐Carbon Nanotube Conjugates for Tissue‐Translatable Glucose Nanosensors

Nishitani,

Tran,

Puglise

et al. 2023

Angew Chem Int Ed

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Continuous and non‐invasive glucose monitoring and imaging is important for disease diagnosis, treatment, and management. However, glucose monitoring remains a technical challenge owing to the dearth of tissue‐transparent glucose sensors. In this study, we present the development of near‐infrared fluorescent single‐walled carbon nanotube (SWCNT) based nanosensors directly functionalized with glucose oxidase (GOx) capable of immediate and reversible glucose imaging in biological fluids and tissues. We prepared GOx‐SWCNT nanosensors by facile sonication of SWCNT with GOx in a manner that—surprisingly—does not compromise the ability of GOx to detect glucose. Importantly, we find by using denatured GOx that the fluorescence modulation of GOx‐SWCNT is not associated with the catalytic oxidation of glucose but rather triggered by glucose‐GOx binding. Leveraging the unique response mechanism of GOx‐SWCNT nanosensors, we developed catalytically inactive apo‐GOx‐SWCNT that enables both sensitive and reversible glucose imaging, exhibiting a ΔF/F0 of up to 40 % within 1 s of exposure to glucose without consuming the glucose analyte. We finally demonstrate the potential applicability of apo‐GOx‐SWCNT in biomedical applications by glucose quantification in human plasma and glucose imaging in mouse brain slices.

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Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring

Abstract: Single-walled carbon nanotubes (SWCNTs) emit photostable near-infrared fluorescence that is conducive for optical glucose monitoring. Such SWCNT-based optical sensors often require the immobilization of proteins that can confer glucose selectivity...

Cited by 12 publications

References 57 publications

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

Sensitive and specific detection of saccharide species based on fluorescence: update from 2016

Engineered Glucose Oxidase‐Carbon Nanotube Conjugates for Tissue‐Translatable Glucose Nanosensors

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