Bioengineering functional copolymers. XII. Interaction of boron-containing and PEO branched derivatives of poly(MA-alt-MVE) with HeLa cells

Türk, Mustafa; Rzayev, Zakir M. O.; Kurucu, Gülcihan

doi:10.4236/health.2010.21009

Cited by 15 publications

(17 citation statements)

References 38 publications

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“…4, no toxic effects were found for any of the experimental groups studied, which is in agreement with the cytocompatibility properties exhibited by PCL 26,27 and PEO. 28 In addition, FGF-2 signicantly increased the cell viability aer 23 h of culture compared to the control ber (-), which could be attributed to the cell mediated survival role of FGF-2 previously described on neural, smooth muscle and endothelial cells. [29][30][31] Cellular attachment and inltration…”

Section: Biocompatibilitymentioning

confidence: 97%

Electrospun PCL/PEO coaxial fibers for basic fibroblast growth factor delivery

Rubert

Dehli

et al. 2014

J. Mater. Chem. B

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The poor innate healing capacity of fibroblastic tissues, such as pelvic floor fascia, is attributed to the scarcity of fibroblasts to produce collagen, as the main collagen producing cells. Coaxial electrospun PCL/PEO fibers containing basic fibroblast growth factor (FGF-2) were evaluated for the local and temporal delivery of FGF-2 for promoting fibroblast proliferation. PCL/PEO coaxial fibers with a highly porous surface were successfully developed using coaxial electrospinning. The diameter of the PCL/PEO microfibers produced by coaxial electrospinning could be tuned by the electrospinning parameters. XPS surface chemistry probing and CA wettability analysis confirmed that the outer surface of the coaxial fibers is PCL. The protein was successfully encapsulated and a sustained release was observed over more than 9 days. In vitro, PCL/PEO coaxial fibers supported fibroblast cell adhesion. In addition, PCL/PEO coaxial fibers containing FGF-2 significantly enhanced fibroblast cell viability and proliferation. Further, Coll-I expression was significantly expressed after day 1 while down-regulated after day 9 compared to the control group. These results indicate that coaxial polymeric fibers allow local and sustained growth factor delivery with prolonged efficacy and longevity for connective tissue regeneration.

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Section: Biocompatibilitymentioning

confidence: 97%

Electrospun PCL/PEO coaxial fibers for basic fibroblast growth factor delivery

Rubert

Dehli

et al. 2014

J. Mater. Chem. B

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“…The number of living and dead cells were counted with a haemacytometer (C.A. Hausse & Son Phluila, USA), using ligth microscope at ×200 magnification 27 . HeLa and fibroblast cells (20 × 10 3 cells per well) were also treated with different amount functional oligomers for 24 h, after removing the medium were stained with hematoxylen/eosin and then images of the cells were recorded by light inverted microscopy (Leica, Germany) 27 .…”

Section: Characterizationmentioning

confidence: 99%

Bioengineering functional copolymers. XV. Synthesis of organoboron amide-ester branched derivatives of oligo(maleic anhydride) and their interaction with HeLa and L929 fibroblast cells

Kahraman¹,

Türk²,

Rzayev³

et al. 2011

Collect. Czech. Chem. Commun.

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Novel bioengineering functional organoboron oligomers were synthesized by (i) amidolysis of oligo(maleic anhydride) (OMA) with 2-aminoethyldiphenylborinate (2-AEPB), (ii) esterification of organoboron oligomer (OMA-B) with α-hydroxy-ω-methoxypoly(ethylene oxide) (PEO) as a compatibilizer and (iii) conjugation of organoboron PEO branches (OMA-B-PEO) with folic acid as a taggering agent. Structure and composition of the synthesized oligomers were characterized by FTIR-ART and 1 H ( 13 C) NMR spectroscopy, chemical and physical analysis methods. Interaction of functional oligomers and oligomer···FA complex (OMA-B-PEO-F) with HeLa and L929 fibroblast cells were investigated by using different biochemical methods such as cytotoxicity, statistical, apoptotic and necrotic cell indexes, double staining and caspase-3 immunostaining, light and fluorescence inverted microscope analyses. It was found that citotoxisity and apoptotic/necrotic effects of oligomers significantly depend on the structure and composition of studied oligomers, and increase the following raw: OMA << OMA-B < OMA-B-PEO < OMA-B-PEO-F. A folic acid complex (MA-PEG-B-F) at 400 µg ml -1 (2.36 µmol ml -1 ) concentration as a therapeutic drug exhibits minimal toxcisity toward the fibroblast cells, but influential for HeLa cells.

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“…With the Hoechst dye, the nuclei of normal cells were stained with blue fluorescence of low intensity, but apoptotic cells were stained a stronger blue fluorescence. The apoptotic cells were also identified by morphological changes in the nucleus including nuclear fragmentation and chromatin condensation (van Engeland et al, 1998;Türk et al, 2010). Nuclei of necrotic cells were stained red by PI, as PI dye can cross the cell membrane of necrotic cells, which lack plasma membrane integrity.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%