Electrospun PCL/PEO coaxial fibers for basic fibroblast growth factor delivery

Rubert, Marina; Dehli, Jeppe; Li, Yanfang; Taskin, Mehmet Berat; Xu, Rui; Besenbacher, Flemming; Chen, Menglin

doi:10.1039/c4tb01258e

Cited by 57 publications

(41 citation statements)

References 37 publications

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“…Likewise, Heybeli et al previously demonstrated that the effect of bFGF on collagen formation was not dose‐dependent in vivo . Indeed, there is a striking discrepancy between the results and our hypothesis based on the in vitro study …”

Section: Discussioncontrasting

confidence: 99%

“…The release kinetics of CTGF from PCL hydrogel and bFGF from hollow fibers have been characterized previously by Xu et al . and Rubert et al ,. respectively.…”

Section: Methodsmentioning

confidence: 95%

“…Approximately 90% CTGF was remained inside the hydrogel to maturize fibrogenic differentiation of the stem cells. On the other hand, 10.6% bFGF was burst released from the hollow fibers during the first hour of incubation and 52.9% was slowly released from day 1 to 6, which was followed by a further slowed down of 8.5% release up to 9 days …”

Section: Methodsmentioning

confidence: 98%

“…Xu et al revealed that PCL fibers containing connective tissue growth factor (CTGF) directed the differentiation of rMSC into fibroblasts in vitro, indicating that the differentiation of rMSC is highly dependent on and controlled by the local microenvironment . In addition, Rubert et al demonstrated that bFGF‐loaded PCL scaffolds increased fibroblast proliferation significantly in vitro . The purpose of this study was to investigate the in vivo effects of six biodegradable PCL meshes delivering either bFGF or CTGF and rMSC on collagen formation in a rat abdominal defect model.…”

Section: Introductionmentioning

confidence: 96%

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Electrospun nanofiber mesh with fibroblast growth factor and stem cells for pelvic floor repair

et al. 2019

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Surgical outcome following pelvic organ prolapse (POP) repair needs improvement. We suggest a new approach based on a tissue‐engineering strategy. In vivo, the regenerative potential of an electrospun biodegradable polycaprolactone (PCL) mesh was studied. Six different biodegradable PCL meshes were evaluated in a full‐thickness abdominal wall defect model in 84 rats. The rats were assigned into three groups: (1) hollow fiber PCL meshes delivering two dosages of basic fibroblast growth factor (bFGF), (2) solid fiber PCL meshes with and without bFGF, and (3) solid fiber PCL meshes delivering connective tissue growth factor (CTGF) and rat mesenchymal stem cells (rMSC). After 8 and 24 weeks, we performed a histological evaluation, quantitative analysis of protein content, and the gene expression of collagen‐I and collagen‐III, and an assessment of the biomechanical properties of the explanted meshes. Multiple complications were observed except from the solid PCL‐CTGF mesh delivering rMSC. Hollow PCL meshes were completely degraded after 24 weeks resulting in herniation of the mesh area, whereas the solid fiber meshes were intact and provided biomechanical reinforcement to the weakened abdominal wall. The solid PCL‐CTGF mesh delivering rMSC demonstrated improved biomechanical properties after 8 and 24 weeks compared to muscle fascia. These meshes enhanced biomechanical and biochemical properties, demonstrating a great potential of combining tissue engineering with stem cells as a new therapeutic strategy for POP repair. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:48–55, 2020.

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Section: Discussioncontrasting

confidence: 99%

“…The release kinetics of CTGF from PCL hydrogel and bFGF from hollow fibers have been characterized previously by Xu et al . and Rubert et al ,. respectively.…”

Section: Methodsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 96%

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Electrospun nanofiber mesh with fibroblast growth factor and stem cells for pelvic floor repair

et al. 2019

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“…So far, in vitro studies show that a polycaprolactone (PCL)/poly ethylene oxide (PEO) mesh with bFGF increases fibroblast cell proliferation with 331% after 9 days of culture, where 72% of the bFGF is released. Furthermore, the PCL/PEO fibers without bFGF have no toxic effect in vitro and added bFGF increases cell viability . The results of the former in vitro study indicated that a PCL mesh with bFGF could be a potential treatment for POP and was chosen to test in vivo .…”

Section: Introductionmentioning

confidence: 99%

Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair?

et al. 2017

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Half of the female population over age 50 years will experience pelvic organ prolapse. We suggest a new approach based on tissue engineering principles to functionally reconstruct the anatomical structures of the pelvic floor. The aim of this study is to investigate the mechanical performance and effect on collagen and elastin production of a degradable mesh releasing basic fibroblast growth factor (bFGF). Implantation of biodegradable mesh with or without bFGF in their core has been conducted in 40 rats in an abdominal wall defect model. Samples were explanted after 4, 8, and 24 weeks, and tested for mechanical properties and the composition of connective tissue. The study showed an increase in mRNA expression for collagen-I (p = 0.0060) and collagen-III (p = 0.0086) in the 4 weeks group with bFGF. The difference was equalized at 8 and 24 weeks. No difference was found at any time for protein amount for collagen-I, collagen-III, and fibronectin. The amount of collagen decreased from 4 to 24 weeks but the fraction of collagen increased. The maximal load of the newly formed tissue showed no effect of bFGF at any time. Exclusively, histology showed a limited ingrowth of collagen fibers after 4 weeks with bFGF but signs of elastin fibers were seen at 24 weeks. The investigation showed that a biodegradable mesh promotes tissue formation with a promising strength. The mesh with bFGF did not represent any advantage on either long or short term in comparison to the mesh without bFGF. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 680-688, 2018.

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Delivery of TGFβ3 from Magnetically Responsive Coaxial Fibers Reduces Spinal Cord Astrocyte Reactivity In Vitro

Funnell,

Fougere,

Zahn

et al. 2024

Advanced Biology

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A spinal cord injury (SCI) compresses the spinal cord, killing neurons and glia at the injury site and resulting in prolonged inflammation and scarring that prevents regeneration. Astrocytes, the main glia in the spinal cord, become reactive following SCI and contribute to adverse outcomes. The anti‐inflammatory cytokine transforming growth factor beta 3 (TGFβ3) has been shown to mitigate astrocyte reactivity; however, the effects of prolonged TGFβ3 exposure on reactive astrocyte phenotype have not yet been explored. This study investigates whether magnetic core‐shell electrospun fibers can be used to alter the release rate of TGFβ3 using externally applied magnetic fields, with the eventual application of tailored drug delivery based on SCI severity. Magnetic core‐shell fibers are fabricated by incorporating superparamagnetic iron oxide nanoparticles (SPIONs) into the shell and TGFβ3 into the core solution for coaxial electrospinning. Magnetic field stimulation increased the release rate of TGFβ3 from the fibers by 25% over 7 days and released TGFβ3 reduced gene expression of key astrocyte reactivity markers by at least twofold. This is the first study to magnetically deliver bioactive proteins from magnetic fibers and to assess the effect of sustained release of TGFβ3 on reactive astrocyte phenotype.

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Electrospun PCL/PEO coaxial fibers for basic fibroblast growth factor delivery

Cited by 57 publications

References 37 publications

Electrospun nanofiber mesh with fibroblast growth factor and stem cells for pelvic floor repair

Electrospun nanofiber mesh with fibroblast growth factor and stem cells for pelvic floor repair

Electrospun biodegradable microfibers induce new collagen formation in a rat abdominal wall defect model: A possible treatment for pelvic floor repair?

Delivery of TGFβ3 from Magnetically Responsive Coaxial Fibers Reduces Spinal Cord Astrocyte Reactivity In Vitro

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