Bioengineering of Male Sterility in Rice (<i>Oryza sativa</i> L.)

Tien, Dung Nguyen; Oo, Moe Moe; Soh, Moon-Soo; Park, Soon Ki

doi:10.9787/pbb.2013.1.3.218

Cited by 8 publications

(4 citation statements)

References 143 publications

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“…Coloring intensity represents the relative value, red shades represent accumulation, whereas blue shades represent relative content decrease. Metabolic pathway scheme according to: [ 45 , 49 , 50 ].…”

Section: Resultsmentioning

confidence: 99%

Changes in Endogenous Phytohormones of Gerbera jamesonii Axillary Shoots Multiplied under Different Light Emitting Diodes Light Quality

2022

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Light quality is essential in in vitro cultures for morphogenesis process. Light emitting diodes system (LED) allows adjustment as desired and the most appropriate light spectrum. The study analyzed the influence of different LED light quality on the balance of endogenous phytohormones and related compounds (PhRC) in in vitro multiplied axillary shoots of Gerbera jamesonii. Over a duration of 40 days, the shoots were exposed to 100% red light, 100% blue light, red and blue light at a 7:3 ratio with control fluorescent lamps. Every 10 days plant tissues were tested for their PhRC content with the use of an ultra-high performance liquid chromatography (UHPLC). Shoots’ morphometric features were analyzed after a multiplication cycle. We identified 35 PhRC including twelve cytokinins, seven auxins, nine gibberellins, and seven stress-related phytohormones. Compounds content varied from 0.00052 nmol/g to 168.15 nmol/g of dry weight (DW). The most abundant group were stress-related phytohormones (particularly benzoic and salicylic acids), and the least abundant were cytokinins (about 370 times smaller content). LED light did not disturb the endogenous phytohormone balance, and more effectively mitigated the stress experienced by in vitro grown plants than the fluorescent lamps. The stress was most effectively reduced under the red LED. Red and red:blue light lowered tissue auxin levels. Blue LED light lowered the shoot multiplication rate and their height, and induced the highest content of gibberellins at the last stage of the culture.

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Section: Resultsmentioning

confidence: 99%

Changes in Endogenous Phytohormones of Gerbera jamesonii Axillary Shoots Multiplied under Different Light Emitting Diodes Light Quality

2022

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“…In the GA biosynthetic pathway of higher plants, GA 3β-hydroxylase catalyzes the conversion of inactive gibberellin precursors (GA 20 , GA 5 and GA 9 ) to bioactive gibberellins (GA 1 , GA 3 and GA 4 ), respectively [38,39]. Bioactive GAs are synthesized by 3-beta-hydroxylation and catalyzed by GA 3β-hydroxylase enzyme in the presence of 2-oxoglutarate binding region, which is essential for its activity [40].…”

Section: Introductionmentioning

confidence: 99%

Molecular Mapping and Candidate Gene Analysis for GA3 Responsive Short Internode in Watermelon (Citrullus lanatus)

Gebremeskel

Dou

et al. 2019

IJMS

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Plants with shorter internodes are suitable for high-density planting, lodging resistance and the preservation of land resources by improving yield per unit area. In this study, we identified a locus controlling the short internode trait in watermelon using Zhengzhouzigua (long internode) and Duan125 (short internode) as mapping parents. Genetic analysis indicated that F1 plants were consistent with long internode plants, which indicates that the long internode was dominant over the short internode. The observed F2 and BC1 individuals fitted the expected phenotypic segregation ratios of 3:1 and 1:1, respectively. The locus was mapped on chromosome 9 using a bulked segregant analysis approach. The region was narrowed down to 8.525 kb having only one putative gene, Cla015407, flanking by CAPS90 and CAPS91 markers, which encodes gibberellin 3β-hydroxylase (GA 3β-hydroxylase). The sequence alignment of the candidate gene between both parents revealed a 13 bp deletion in the short internode parent, which resulted in a truncated protein. Before GA3 application, significantly lower GA3 content and shorter cell length were obtained in the short internode plants. However, the highest GA3 content and significant increase in cell length were observed in the short internode plants after exogenous GA3 application. In the short internode plants, the expression level of the Cla015407 was threefold lower than the long internode plants in the stem tissue. In general, our results suggested that Cla015407 might be the candidate gene responsible for the short internode phenotype in watermelon and the phenotype is responsive to exogenous GA3 application.

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“…Some TMS rice lines have been developed (Abe et al 2018, Akasaka et al 2018, Lu et al 2000, but their flowering habits are not suitable for RS-based breeding because of their delayed heading, flowering, and daily flowering time relative to the wild type (Abe et al 2018). Similar delays were observed in cytoplasmic male sterility (MS) in hybrid rice, which caused failure of opening of 20% of female parent spikelets (Yan and Li 1987), partial or full panicle enclosure (Singh and Shirisha 2003, Tien et al 2013, Yin et al 2007, and asynchronous flowering (Lou et al 2014). These features decrease outcrossing fertility.…”

Section: Construction Of Populations For Genetic Analysismentioning

confidence: 99%

Detection of dominant QTLs for stigma exsertion ratio in rice derived from <i>Oryza rufipogon</i> accession ‘W0120’

Bakti

Tanaka

2019

Breed. Sci.

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Stigma exsertion can enhance the outcrossing fertility in rice. Dominant genes for this trait are expected to be essential for the effective application of a novel outcrossing-based breeding system that uses male sterility in rice, which is normally autogamous. Because reduction of stigma exsertion is a domestication trait, we screened wild rice species as possible donors of genes or QTLs for stigma exsertion. We used in silico image-based screening and selected the Oryza rufipogon accession 'W0120'. A single F 1 individual derived from a cross between the japonica rice cultivar 'Akidawara' and 'W0120' was used to generate F 2 and BC 1 F 1 populations. QTL analysis performed using 114 F 2 individuals detected QTLs on chromosomes 2, 3, 4, 8, and 11. Only two major QTLs on chromosomes 3 and 8 showed higher degrees of dominance. On the other hand, there were no QTLs near GS3, which is well known as a gene for stigma exsertion. Validation of these QTLs using 188 BC 1 F 1 individuals provided clear evidence for their dominance. Genotypes of the markers nearest to the two QTLs were also related to grain length. We expect the genes responsible for these QTLs to be promising tools for improving outcrossing-based breeding in rice.

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Bioengineering of Male Sterility in Rice (Oryza sativa L.)

Cited by 8 publications

References 143 publications

Changes in Endogenous Phytohormones of Gerbera jamesonii Axillary Shoots Multiplied under Different Light Emitting Diodes Light Quality

Changes in Endogenous Phytohormones of Gerbera jamesonii Axillary Shoots Multiplied under Different Light Emitting Diodes Light Quality

Molecular Mapping and Candidate Gene Analysis for GA3 Responsive Short Internode in Watermelon (Citrullus lanatus)

Detection of dominant QTLs for stigma exsertion ratio in rice derived from <i>Oryza rufipogon</i> accession ‘W0120’

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