2015
DOI: 10.1016/j.actbio.2015.09.002
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Bioengineering vascularized tissue constructs using an injectable cell-laden enzymatically crosslinked collagen hydrogel derived from dermal extracellular matrix

Abstract: Tissue engineering promises to restore or replace diseased or damaged tissue by creating functional and transplantable artificial tissues. The development of artificial tissues with large dimensions that exceed the diffusion limitation will require nutrients and oxygen to be delivered via perfusion instead of diffusion alone over a short time period. One approach to perfusion is to vascularize engineered tissues, creating a de novo three-dimensional (3D) microvascular network within the tissue construct. This … Show more

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Cited by 89 publications
(56 citation statements)
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“…For tissue engineering application of large‐sized tissue‐like constructs, appropriate levels of oxygen and nutrients are needed to ensure cell survival. Otherwise, decrease in the cell viability can finally cause the failure of tissue regeneration or the necrosis of tissues . Porous 3D biomaterials for cell encapsulation have been developed to enhance the delivery of nutrients.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…For tissue engineering application of large‐sized tissue‐like constructs, appropriate levels of oxygen and nutrients are needed to ensure cell survival. Otherwise, decrease in the cell viability can finally cause the failure of tissue regeneration or the necrosis of tissues . Porous 3D biomaterials for cell encapsulation have been developed to enhance the delivery of nutrients.…”
Section: Resultsmentioning
confidence: 99%
“…Cell-laden hydrogels can be assembled into constructs mimicking natural tissue structures or directly injected into injured sites to fill the tissue defect with irregular shapes. 10,11 In neural tissue engineering, researchers have developed a number of hydrogels (e.g., alginate, 12 collagen, 13 gelatin, 9 and self-assembling peptides 14 ) to encapsulate and release different types of cells (e.g., neural stem cells, 14 mesenchymal stem cells, 15 olfactory ensheathing cells, 16 and Schwann cells 17 ). Cell-laden hydrogels can be injected to the injury epicenter to fill the cavity or combined with nerve conduit to bridge the lesion gap, resulting in axon regeneration and functional recovery.…”
Section: Introductionmentioning
confidence: 99%
“…Many types of cells are being administered via subcutaneous injection within different kinds of solutions composed of hyaluronic acid, PRP, collagen, chitosan, poly(ethylene glycol)‐poly( l ‐alanine), ECM or methylcellulose, among others . Moreover, cells can be applied mixed with injectable hydrogels that provide a scaffold for in situ tissue regrowth and regeneration, allowing surgeons to fill complex shapes with a minimal invasive procedure .…”
Section: Therapeutic Strategies For Skin Regenerationmentioning
confidence: 99%
“…Many types of cells are being administered via subcutaneous injection within different kinds of solutions composed of hyaluronic acid, PRP, collagen, chitosan, poly(ethylene glycol)poly(L-alanine), ECM or methylcellulose, among others. 50,[55][56][57][58][59] Moreover, cells can be applied mixed with injectable hydrogels that provide a scaffold for in situ tissue regrowth and regeneration, allowing surgeons to fill complex shapes with a minimal invasive procedure. 60,61 Several biomaterials have been employed for developing injectable hydrogels to mimic the biological cues of native ECM, however, they cannot reproduce the complex functions of natural ECM.…”
Section: Injectable Cell Solutionsmentioning
confidence: 99%
“…This significantly shortens the time of in vivo anastomosis, perfusion and graft integration with the host. 59 For decellularization various combinations of physical, chemical, and enzymatic treatments are carefully given to isolate ECM scaffold. These treatments help to maintain the structural and chemical integrity of the original tissue.…”
Section: De-cellularizationmentioning
confidence: 99%