Biofabrication of Cell‐Loaded 3D Spider Silk Constructs

Schacht, Kristin; Jüngst, Tomasz; Schweinlin, Matthias; Ewald, Andrea; Groll, Jürgen; Scheibel, Thomas

doi:10.1002/anie.201409846

Cited by 218 publications

(210 citation statements)

References 43 publications

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“…The storage moduli (G′) exceeded the loss moduli (G″) over the whole angular frequency range (G′ > G″), and both moduli were slightly dependent on frequency, indicating a typical gel structure under these conditions. [30] Figure 4B illustrates the final storage modulus for the four bioink compositions. The 10% (w/v) bioink had the highest final storage modulus (258 ± 2.5 kPa) while the 2.5% (w/v) bioink had the lowest (196 ± 3.0 Pa), suggesting that storage modulus was highly silk concentration dependent, and high concentration silk/PEG bioinks would be more preferred for 3D bioprinting due to better self-standing features than low concentration bioinks.…”

Section: Rheological Propertiesmentioning

confidence: 99%

3D Bioprinting of Self‐Standing Silk‐Based Bioink

Zheng

et al. 2018

Adv Healthcare Materials

203

127

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Silk/polyethylene glycol (PEG) hydrogels are studied as self‐standing bioinks for 3D printing for tissue engineering. The two components of the bioink, silk fibroin protein (silk) and PEG, are both Food and Drug Administration approved materials in drug and medical device products. Mixing PEG with silk induces silk β‐sheet structure formation and thus gelation and water insolubility due to physical crosslinking. A variety of constructs with high resolution, high shape fidelity, and homogeneous gel matrices are printed. When human bone marrow mesenchymal stem cells are premixed with the silk solution prior to printing and the constructs are cultured in this medium, the cell‐loaded constructs maintain their shape over at least 12 weeks. Interestingly, the cells grow faster in the higher silk concentration (10%, w/v) gel than in lower ones (7.5 and 5%, w/v), likely due to the difference in material stiffness and the amount of residual PEG remaining in the gel related to material hydrophobicity. Subcutaneous implantation of 7.5% (w/v) bioink gels with and without printed fibroblast cells in mice reveals that the cells survive and proliferate in the gel matrix for at least 6 week postimplantation. The results suggest that these silk/PEG bioink gels may provide suitable scaffold environments for cell printing and function.

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Section: Rheological Propertiesmentioning

confidence: 99%

3D Bioprinting of Self‐Standing Silk‐Based Bioink

Zheng

et al. 2018

Adv Healthcare Materials

203

127

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“…21 Schacht et al developed shear-thinning bioink hydrogels based on recombinant spider silk proteins that do not require additional components or postprocessing to produce 3D-printed cell-laden constructs with a good printing fidelity and cytocompatibility. 22 The Burdick research group also proposed an interesting printing strategy based on hyaluronic acid (HA) bioink that crosslinks through supramolecular assembly of guest-host complexes. 23 HA was modified with either adamantane (Ad) or b-cyclodextrin (b-CD) pending moieties that result in shear-thinning and self-healing hydrogels on mixing.…”

Section: New Bioink Biomaterialsmentioning

confidence: 99%

Tissue Engineering and Regenerative Medicine: New Trends and Directions—A Year in Review

Gomes

Rodrigues

Domingues

et al. 2017

Tissue Engineering Part B: Reviews

151

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Tissue engineering (TE) is continuously evolving assimilating inputs from adjacent scientific areas and their technological advances, including nanotechnology developments that have been spawning the range of available options for the precise manipulation and control of cells and cellular environments. Simultaneously, with the maturation of the field, TE has a growing and marked impact in other fields, such as cancer and other diseases research, enabling tri-dimensional (3D) tumor/tissue models of increased complexity that more closely resemble living tissue dynamics, playing a decisive role in the development of new and improved therapies. Nevertheless, TE is still struggling with translational issues. On this matter, the advent of personalized and precision medicine has opened new perspectives, particularly with the striking evolutions enabled by 3D bioprinting technologies. Based on a modified methodology grounded in the past years' approach, we have identified and reviewed some of the most high-impact publications on the topics that are revolutionizing TE and helping to define the future directions of the field, namely: (1) New trends in TE: Personalized/precision regenerative medicine and 3D bioprinting, (2) Contributions of TE to other fields: microfabricated tissueengineered 3D models for cancer and other diseases research, and (3) Diagnostic and theranostic tools: monitoring and real-time control of TE systems.Keywords: 3D bioprinting, 3D disease models, nanotechnology, precision regenerative medicine, theranostic toolsThe Aim, Scope, and Methods of This Review S ince its origin, tissue engineering (TE) holds the promise of revolutionizing healthcare by providing artificially developed tissues and organs substitutes on demand. More recently, TE has expanded into different biomedical areas to feedback research and clinical needs, widening the range not only of possible successful therapies but also of diagnostic/screening tools. Considering the growing number of publications related with TE and approaching different scientific domains, such as cancer science or pharmaceutics research, the potential complementary role of the field in a multitude of clinical areas and therapies in the years to come is evident.Our review attempts to cover some of the most exciting research contributing to both regenerative medicine and in vitro research applications of engineered tissues, along with the novel technological approaches that are advancing the field. This is the fifth review of the kind in TE. The previous four articles [1][2][3][4] helped to establish the methodology adopted for selecting the areas of focus and the contributions to highlight. As in previous years, we started by searching the ISI Web of Knowledge database for articles in ''tissue engineering'' and ''regenerative medicine'' published during the period of September 2015 through December 2016, thus using a 3-month overlap with the previous review to not miss impactful articles in areas not addressed earlier.Similar to previous years, we also consid...

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“…Gelation can happen without any important secondary structural changes; intramolecular cross-linking between protein chains happens with the aid of electrostatic interaction, hydrogen bonds and hydrophobic interactions, forming strong β-sheets [26]. The gelation time can be shortened with the aid of physical changes as lowering the pH [27], increasing the Temperature [28], sonication [29] or by adding chemical crosslinking agents [30]. Silk is a good material for bioprinting, as it has good mechanical stability [25,31], and has shown to allow cells to attach and proliferate [32].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

“…Schacht et al have shown that recombinant silk can be used for 3D bioprinting by letting a silk-cell mixture gel overnight before using them with extrusion printing. By incorporating the cell adhesion peptide motif RGD they were able to increase cell adhesion and proliferation [28]. The same group showed that this silk biomaterial has enough mechanical strength to print larger, more intricate structures [34].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Current developments in 3D bioprinting for tissue engineering

Cornelissen

Faulkner-Jones

Shu

2017

Current Opinion in Biomedical Engineering

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This version is available at https://strathprints.strath.ac.uk/61660/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPTCurrent developments in 3D bioprinting for tissue engineering AbstractThe field of 3-dimensional (3D) bioprinting have enjoyed rapid development in the past few years for the applications in tissue engineering and regenerative medicine. In this review, we summarize the most updated developments in 3D bioprinting for the applications in the tissue engineering with a focus on the printable biomaterials used as bioinks. These developments include 1) novel printing regimes have been enabled by the use of fugitive inks for the creation of intricate structures e.g. vascularized tissue constructs; 2) mechanical strength of printed constructs can be enhanced by co-printing soft and hard biomaterials; 3) bioprinted in-vitro models for drug testing applications are closer to reality. We conclude that the research and application of new bioinks will remain the key highlights of the future developments in 3D bioprinting for tissue engineering.

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Biofabrication of Cell‐Loaded 3D Spider Silk Constructs

Cited by 218 publications

References 43 publications

3D Bioprinting of Self‐Standing Silk‐Based Bioink

3D Bioprinting of Self‐Standing Silk‐Based Bioink

Tissue Engineering and Regenerative Medicine: New Trends and Directions—A Year in Review

Current developments in 3D bioprinting for tissue engineering

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