Biofilm as a virulence marker in Candida species in Nosocomial blood stream infection and its correlation with antifungal resistance

Bhatt, Majid Hassan; Sarangi, Gitanjali; Paty, BP; Mohapatra, Debahuti; Chayani, Nirupama; Mahapatra, Ashoka; Das, Padma; Sahoo, Debasish

doi:10.4103/0255-0857.150909

Cited by 31 publications

(27 citation statements)

References 14 publications

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“…In the last few years, occurrence of NAC species is steadily increasing. In the present study, NAC accounted for 70% similar to studies by Oberoi et al, [11] 83.2% and Bhatt et al, [12] 85.3%. Over the past 20 years, a shift toward NAC species has been reported previously from the USA, Europe, and Australia although the precise pattern of causative species varies across countries.…”

Section: Discussionsupporting

confidence: 91%

“…Secondary resistance in C. albicans is primarily due to a decrease in the activity of the uracil phosphoribosyltransferase. [27][28][29] Our study showed 81% sensitivity to flucytosine which was similar to 63% by Bhatt et al [12] and 88.5% by Vinodkumar et al [30] The incidence of amphotericin B-resistant Candida spp. in our study was 13% which is comparable to incidences reported in recent studies.…”

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

Prevalence of non-albicans candidemia in a tertiary care hospital in Northeast India

Sabhapandit¹,

Lyngdoh²,

Bora³

et al. 2017

Int J Med Sci Public Health

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 83%

Prevalence of non-albicans candidemia in a tertiary care hospital in Northeast India

Sabhapandit¹,

Lyngdoh²,

Bora³

et al. 2017

Int J Med Sci Public Health

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“…And as reported by other authors, the MIC obtained in this study regarding flucytosine in the 15 strains analyzed is high compared to that reported by Silva et al, [3] Miranda et al, [2] and Canton et al [17]. These reports were obtained in studies conducted in Europe, while in India, Bhatt M et al [22]obtained high rates of FLC resistance in C. parapsilosis isolates.…”

Section: Discussionsupporting

confidence: 71%

Molecular Characterization of Candida parapsilosis Species Complex in Niches of the Oral Cavity in a Cohort of Patients from Argentina with Different Oral and Dental Clinical Manifestations

Rodríguez¹

2016

J. Dent. Sci. Ther

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Candida parapsilosis is considered to be a complex of three species due to genetic heterogeneity. Currently, it is the second most isolated yeast in bloodstream infections and oral cavity sites with C. parapsilosis sensu stricto and C. metapsilosis being the species mostly recovered from oral niches in both healthy and immunecompromised patients respectively. Disparity in antifungal susceptibility has been reported among species of this complex, while no data on distribution and behavior in the oral cavity has been reported in Argentina.Objective: To molecularly characterize a collection of isolates from Argentina, phenotypically identified as C. parapsilosis from different oral niches, in a cohort of patients with different oral and dental clinical conditions. Methodology:This experimental study used 31 yeasts phenotypically recognized as C. parapsilosis and recovered for molecular characterization by end-point PCR using specific primers. The in vitro sensitivity of isolates against azoles, echinocandins, amphotericin and flucytosine was evaluated by automated Vitek2 system and MIC (Minimum Inhibitory Concentration) in line with CLSI (Clinical and Laboratory Standards Institute) M27-A3 guidelines.Results: All isolates, 100% (31/31), were positive for C. parapsilosis sensu stricto species. Of these, 77.4% were recovered in oral inflammatory conditions, and 94.7% of the strains analyzed for antifungal susceptibility profile showed favorable response to all drugs tested. Conclusions:C. parapsilosis sensu strictois a common colonizing of the oral mucosa under pathological conditions, and generally shows favorable response to antifungal agents commonly used in clinical practice, but with a tendency to lower responsiveness to azoles and echinocandins.

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“…However, Shin et al [23] reported C. tropicalis was most common isolates followed by C. parapsilosis, C. glabrata, and C. albicans. While, another study by Bhatt et al [29] reported C. parapsilosis and C. tropicalis were strong biofilm producers whereas C. albicans and C. krusei were identified as weak producers.…”

Section: Discussionmentioning

confidence: 95%

“…Yet, glucose is thought to serve as the carbohydrate energy source required by Candida for biofilm formation [25], perhaps necessary to produce the polysaccharide matrix [27], in which organized communities of yeast, hyphae, and pseudohyphae are enclosed [28]. In a study by Bhatt et al [29] they reported administration of broad spectrum antibiotics, indwelling catheter and patients on mechanically ventilator were the major risk factor.…”

Section: Discussionmentioning

confidence: 99%

Detection of biofilm production in Candida species isolates recovered from bloodstream patients

Pal¹,

Bisht²

2016

Int J of Biomed & Adv Res

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Introduction: Candida Bloodstream Infections (CBSIs) are the fourth most common infections among hospitalized patients. Candida biofilm results from an initial attachment of cells to glycoprotein-coated host cells and tissue or biomaterial surfaces. Material and Method: Candida spp. was identified by conventional method and biofilm formation was detected by tube method. Result: C. tropicalis was the most common species followed by C. haemulonii, C. albicans, C. parapsilosis, C. glabrata, C. pelliculosa, C. guilliermondii and C. krusei. 125 of the isolates were tested for biofilm formation of which 67(53.6%) were found to be capable of forming biofilms. Of these 55(82%) biofilm forming isolates were those recovered from patients with Catheter Related Candidemia (CRC) while 12 (17.9%) biofilm forming isolates were without CRC. Most common biofilm producing species were C.pelliculosa followed by C.tropicalis, C.haemulonii, C. parapsilosis, C.glabrata, C. albicans, C.krusei and C. guilliermondii. Conclusion: Our study found more biofilm production in Central Venous Catheter (CVC) Candidemia and most of the CVC related Candidemia were found in diabetic and neutropenic patients and hence care should be taken in those patients who are at risk of developing biofilm production before applying the external appliances.

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Biofilm as a virulence marker in Candida species in Nosocomial blood stream infection and its correlation with antifungal resistance

Cited by 31 publications

References 14 publications

Prevalence of non-albicans candidemia in a tertiary care hospital in Northeast India

Prevalence of non-albicans candidemia in a tertiary care hospital in Northeast India

Molecular Characterization of Candida parapsilosis Species Complex in Niches of the Oral Cavity in a Cohort of Patients from Argentina with Different Oral and Dental Clinical Manifestations

Detection of biofilm production in Candida species isolates recovered from bloodstream patients

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