Nosocomial blood stream infections (BSI) due to fungi especially Candida is increasing steadily. A two year prospective study was conducted in the S.C.B. Medical College with an aim to evaluate the species distribution, antifungal susceptibility and biofilm formation of Candida spp. isolated from nosocomial BSIs. 34 Candida spp. were isolated from 359 blood cultures. Antifungal susceptibility was performed by microbroth dilution technique and both visual and spectrophotometric method were used for biofilm detection. C. tropicalis was the common spp. isolated followed by C. parapsilosis and others. Most (92%) of the isolates were susceptible to Amphoterecin-B and highest resistance was observed against Flucytosine (37%) and Fluconazole(35%). Biofilm production and antifungal resistance was observed more in nonalbicans Candida spp.

Present study was conducted to find out the pathogenicity of three species of fungi,viz. Aspergillus niger, Aphanomyces laevis and Saprolegnia parasitica isolated from Gold fish (Carrasius auratus) collected from pet shops. Experiment was conducted for 10 days period with concentration of 8x10 3 conidia/ml (A.niger), 125 zoospores/ml (A.laevis) and 2x10 3 zoospores /ml (S.parasitica). All the tested fungi were found pathogenic to fish. Among the three species of fungi tested, A.laevis and S.parasitica showed 100% mortality in fish while A.niger showed 75% mortality. S.parasitica was found most virulent causing mortality within six days of experiment. Histopathological examination of fishes showed inflammation of epidermis , loss of epidermis and necrotized hypodermis. Degenerative changes were observed in musculature. Ulcerated skin showed mycotic granulommas.