Biofilm-degrading enzymes from<i>Lysobacter gummosus</i>

Gökçen, Anke; Vilcinskas, Andreas; Wiesner, Jochen

doi:10.4161/viru.27919

Cited by 35 publications

(25 citation statements)

References 55 publications

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“…13 Lysobacter gummosus has also recently been reported to produce peptidases, which degrade biofilms formed by Staphylococcus epidermidis. 19 In this study, we examined the use of marine derived lipases as potential novel sources of antibiofilm agents and report on the characterization of a new halotolerant lipase isolated from Oceanobacillus sp. PUMB02.…”

Section: Introductionmentioning

confidence: 99%

A halotolerant thermostable lipase from the marine bacteriumOceanobacillussp. PUMB02 with an ability to disrupt bacterial biofilms

et al. 2014

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A halotolerant thermostable lipase was purified and characterized from the marine bacterium Oceanobacillus sp. PUMB02. This lipase displayed a high degree of stability over a wide range of conditions including pH, salinity, and temperature. It was optimally active at 30 °C and pH 8.0 respectively and was stable at higher temperatures (50-70 °C) and alkaline pH. The molecular mass of the lipase was approximately 31 kDa based on SDS-PAGE and MALDI-ToF fingerprint analysis. Conditions for enhanced production of lipase by Oceanobacillus sp. PUMB02 were attained in response surface method-guided optimization with factors such as olive oil, sucrose, potassium chromate, and NaCl being evaluated, resulting in levels of 58.84 U/ml being achieved. The biofilm disruption potential of the PUMB02 lipase was evaluated and compared with a marine sponge metagenome derived halotolerant lipase Lpc53E1. Good biofilm disruption activity was observed with both lipases against potential food pathogens such as Bacillus cereus MTCC1272, Listeria sp. MTCC1143, Serratia sp. MTCC4822, Escherichia coli MTCC443, Pseudomonas fluorescens MTCC1748, and Vibrio parahemolyticus MTCC459. Phase contrast microscopy, scanning electron microscopy, and confocal laser scanning microscopy showed very effective disruption of pathogenic biofilms. This study reveals that marine derived hydrolytic enzymes such as lipases may have potential utility in inhibiting biofilm formation in a food processing environment and is the first report of the potential application of lipases from the genus Oceanobacillus in biofilm disruption strategies.

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Section: Introductionmentioning

confidence: 99%

A halotolerant thermostable lipase from the marine bacteriumOceanobacillussp. PUMB02 with an ability to disrupt bacterial biofilms

et al. 2014

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“…Proteolytic activity is one of the characteristics shared by Lysobacter members that has received most attention, because of its possible application to research and industrial processes (Gökçen et al . ). As regards the control of phytopathogenic micro‐organisms, these enzymes may be exploited for the control of phytopathogenic bacteria such as Erwinia carotovora (Vasilyeva et al .…”

Section: Involvement Of Lytic Enzymes In the Biological Control Of Phmentioning

confidence: 97%

The impact of the omics era on the knowledge and use ofLysobacterspecies to control phytopathogenic micro-organisms

2017

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Omics technologies have had a tremendous impact on underinvestigated genera of plant disease biocontrol agents such as Lysobacter. Strong evidence of the association between Lysobacter spp. and the rhizosphere has been obtained through culture-independent methods, which has also contributed towards highlighting the relationship between Lysobacter abundance and soil suppressiveness. It is conceivable that the role played by Lysobacter spp. in soil suppressiveness is related to their ability to produce an impressive array of lytic enzymes and antibiotics. Indeed, genomics has revealed that biocontrol Lysobacter strains share a vast number of genes involved in antagonism activities, and the molecular pathways underlying how Lysobacter spp. interact with the environment and other micro-organisms have been depicted through transcriptomic analysis. Furthermore, omics technologies shed light on the regulatory pathways governing cell motility and the biosynthesis of antibiotics. Overall, the results achieved so far through omics technologies confirm that the genus Lysobacter is a valuable source of novel biocontrol agents, paving the way for studies aimed at making their application in field conditions more reliable.

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“…Inhibition and (or) dispersal of microbial biofilms could lead to improved antimicrobial efficacy. Genomic technologies augment biofilm research via improved understanding of biofilm complexity and community composition (Sauer 2000), and can assist in the identification of therapeutic agents that degrade (Gökçen et al 2014) or inhibit the formation of biofilms. One strategy to mitigate biofilms has been to utilize antibodies raised against sequence-identified proteins involved in biofilm formation.…”

Section: Biofilm Inhibition and Dispersalmentioning

confidence: 99%

Genomic approaches to characterizing and reducing antimicrobial resistance in beef cattle production systems

Klima

Cameron²,

Javed³

et al. 2017

Can. J. Anim. Sci.

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Antimicrobial resistance (AMR) is a global health threat, and a standstill in the discovery and design of new antibiotics has been linked to the growing number of human deaths attributed to AMR infections. Intensive beef production utilizes antimicrobials to promote health and growth efficiency. To understand the magnitude and risk of AMR in beef production, it is important to assess the prevalence and diversity of antimicrobial resistant genes (ARGs) within microbial populations. Antimicrobial resistant bacteria are traditionally identified by isolation and growth in the presence of selective antibiotics. Whole-genome, metagenomic, and RNA sequencing provide new avenues to detect and identify novel ARGs in both culturable and unculturable bacterial communities. Some of these approaches place ARGs within the context of mobile genetic elements, gauging their likelihood of transfer across genomes. Genomics can also mitigate AMR, contributing to rational drug design or the development of alternatives to antimicrobials such as vaccines and probiotics. RNA-seq-based transcriptomics and Tn-seq may provide new ways to examine mechanisms that promote or prevent AMR. Finally, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas gene editing could directly reduce AMR by killing AMR-resistant bacteria without harming beneficial bacteria. Together, these technologies may provide new opportunities to identify, quantify, and mitigate AMR while developing alternatives to antimicrobials for beef production.

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Biofilm-degrading enzymes fromLysobacter gummosus

Cited by 35 publications

References 55 publications

A halotolerant thermostable lipase from the marine bacteriumOceanobacillussp. PUMB02 with an ability to disrupt bacterial biofilms

A halotolerant thermostable lipase from the marine bacteriumOceanobacillussp. PUMB02 with an ability to disrupt bacterial biofilms

The impact of the omics era on the knowledge and use ofLysobacterspecies to control phytopathogenic micro-organisms

Genomic approaches to characterizing and reducing antimicrobial resistance in beef cattle production systems

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