Biofilm detection and the clinical significance ofStaphylococcus epidermidis isolates

Růžička, Filip; Holá, Veronika; Votava, Miroslav; Tejkalová, Renata; Horvat, Rebecca T.; Heroldová, Monika Dvořáková; Woznicová, Vladana

doi:10.1007/bf02931540

Cited by 58 publications

(27 citation statements)

References 19 publications

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“…On the other hand, many experiments have shown the low usefulness of the CRA method for biofilm production analysis and this was also confirmed in the presented work where such a coincidence was not observed. Phenotypic methods for biofilm formation detection are among the easy and cheap techniques available for routine laboratory use, but may cause some difficulties in result interpretation since they can be influenced by variations in medium composition and cultivation conditions and are prone to subjective errors [36]. …”

Section: Resultsmentioning

confidence: 99%

Biofilm Formation and Antimicrobial Susceptibility of Staphylococcus epidermidis Strains from a Hospital Environment

Wojtyczka

Orlewska

Kępa

et al. 2014

IJERPH

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The hospital environment microflora comprise a wide variety of microorganisms which are more or less pathogenic and where staphylococci are one of the most common types. The aim of the presented study was to evaluate the prevalence of the biofilm forming coagulase-negative staphylococci (CoNS) in a hospital environment as a risk factor for nosocomial infections. Among 122 isolated and tested strains of CoNS the most frequent were: S. epidermidis—32 strains, S. haemolyticus—31 strains, S. capitis subsp. capitis—21 strains, S. hominis—11 strains, S. cohnii subsp. cohnii—nine strains. In case of CoNS, the main molecule responsible for intercellular adhesion is a polysaccharide intercellular adhesin (PIA), encoded on the ica gene operon. The analysis revealed the presence of the icaADBC operon genes in 46.88% of S. epidermidis isolates. IcaA and icaD were present in 34.38% and 28.13% of strains respectively while IcaC gene was present in 37.50% of strains. IcaB gene was found in 21.88% of S. epidermidis strains. In 15 (63%) strains all icaADBC operon genes were observed. The assessment of antibacterial drugs susceptibility demonstrated that analyzed CoNS strains were highly resistant to macrolides and lincosamides and more sensitive to rifampicin and linezolid. Our data indicates that the hospital environment can be colonized by biofilm forming coagulase-negative staphylococci and transmission of these strains can cause an increased risk of serious nosocomial infections.

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Section: Resultsmentioning

confidence: 99%

Biofilm Formation and Antimicrobial Susceptibility of Staphylococcus epidermidis Strains from a Hospital Environment

Wojtyczka

Orlewska

Kępa

et al. 2014

IJERPH

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“…However, some studies found no association between icaD detection and biofilm formation; the presence of icaA/D genes was not always associated with in vitro formation of biofilm [7]. Ruzicka et al demonstrated that 20% of strains with ica genes did not express phenotype [21]. The lack of biofilm, despite the presence of ica, could be due to several reasons, such as the inactivation of the ica operon by the action of the icaR repressor [22], or the post-transcriptional regulation [23].…”

Section: Discussionmentioning

confidence: 99%

Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco

Serray

Oufrid

Hannaoui

et al. 2016

J Infect Dev Ctries

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Introduction: Infections involving methicillin-resistant Staphylococcus aureus (MRSA) remain a serious threat to hospitalized patients worldwide. MRSA is characterized by recalcitrance to antimicrobial therapy, which is a function not only of widespread antimicrobial resistance, but also the capacity to form biofilms. The present study evaluated the presence of genes encoding adhesion factors and the biofilmforming capacity in MRSA. Methodology: In this study, 53 isolates of MRSA, recovered from December 2010 to May 2014 in a mother and child hospital, CHU Mohamed VI in Marrakech, Morocco, were screened for the presence of bap and ica genes associated with biofilm formation, and for bbp, cna, ebpS, eno, fib, fnbA, fnbB, clfA, and clfB genes that encode microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). The biofilm formation assay was performed in 96-well microtiter polystyrene plates. The presence of genes was determined by polymerase chain reaction (PCR). Results: An association was found between icaD gene detection and biofilm formation; 100% of the strains harbored icaD and produced biofilm. None of the isolates harbored bap or bbp. Furthermore, 96.23% isolates were positive for fnbA, 60.37% for eno, 43.39% for clfA and clfB, 11.32% for cna, 9.34% for ebpS, 5.66% for fib, and 1.89% for fnbA. Conclusions: Our findings showed that the MRSA carriage in Marrakech children was high. The genetic variations of adhesion genes require further investigation.

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“…Thus, the prevention of biofilm formation by such natural lactobacilli-derived agents is one possible approach, which seems to be a very attractive idea of novel therapy currently tested (Singh and Cameotra 2004;Rodrigues et al 2006b). Biofilms are involved in the pathogenesis of various infections, and staphylococci are an example of bacteria which are very potent biofilm-producers (Goetz 2002;Růžička et al 2004). …”

Section: Abbreviationsmentioning

confidence: 99%

The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation

et al. 2008

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The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and image PHLIP analysis. The number of adhering S. aureus and S. epidermidis cells after a 3-h co-incubation with biosurfactants was reduced by 5-56 % in a strain-and dose-dependent manner. S. epidermidis-and, to a lower extent, in S. aureus-biofilm formation was also inhibited in the presence of the tested surfactants. The addition of surfactants to preformed mature biofilms accelerated their dispersal, and changed the parameters of biofilm morphology. The L. acidophilus-derived surfactants inhibit bacterial deposition rate and biofilm development (and also its maturation) without affecting cell growth probably due to the influence on the cell-surface hydrophobicity of staphylococci.

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Biofilm detection and the clinical significance ofStaphylococcus epidermidis isolates

Cited by 58 publications

References 19 publications

Biofilm Formation and Antimicrobial Susceptibility of Staphylococcus epidermidis Strains from a Hospital Environment

Biofilm Formation and Antimicrobial Susceptibility of Staphylococcus epidermidis Strains from a Hospital Environment

Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco

The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation

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