Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco

Abstract

Introduction: Infections involving methicillin-resistant Staphylococcus aureus (MRSA) remain a serious threat to hospitalized patients worldwide. MRSA is characterized by recalcitrance to antimicrobial therapy, which is a function not only of widespread antimicrobial resistance, but also the capacity to form biofilms. The present study evaluated the presence of genes encoding adhesion factors and the biofilmforming capacity in MRSA. Methodology: In this study, 53 isolates of MRSA, recovered from December 2010 to May 2014 in a mother and child hospital, CHU Mohamed VI in Marrakech, Morocco, were screened for the presence of bap and ica genes associated with biofilm formation, and for bbp, cna, ebpS, eno, fib, fnbA, fnbB, clfA, and clfB genes that encode microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). The biofilm formation assay was performed in 96-well microtiter polystyrene plates. The presence of genes was determined by polymerase chain reaction (PCR). Results: An association was found between icaD gene detection and biofilm formation; 100% of the strains harbored icaD and produced biofilm. None of the isolates harbored bap or bbp. Furthermore, 96.23% isolates were positive for fnbA, 60.37% for eno, 43.39% for clfA and clfB, 11.32% for cna, 9.34% for ebpS, 5.66% for fib, and 1.89% for fnbA. Conclusions: Our findings showed that the MRSA carriage in Marrakech children was high. The genetic variations of adhesion genes require further investigation.

Molecular analysis, biofilm formation, and susceptibility of methicillin-resistant Staphylococcus aureus strains causing community- and health care-associated infections in central venous catheters

“…A study conducted in Morocco revealed adhesion genes prevalence rates of 96% fnbA, 60% eno, 43% clfA, 43% clfB, 11% cna, 6% fib, and 2% fnbB in MRSA isolated from clinical specimens. These results contradicted our findings because these studies used different specimens to isolate the pathogens 58,60 . The results of the prevalence of adhesion genes among MRSA agrees with those of previous studies 61,62 .…”

“…Interestingly, a significant difference was observed between the strong biofilm producers CA-MRSA and HA-MRSA because of the adhesion genes evaluated in this study (p < 0.01). Twenty-five isolates contained all of the adhesion genes, suggesting that adhesion genes were exclusively involved in biofilm formation 58 . No gene or set of genes can function as a sole indicator of biofilm formation potential; this outcome supports previous findings 59 .…”

icaA/D Genes and Biofilm Formation of Methicillin-Resistant Staphylococcus aureus in Dr. Soetomo Hospital, Surabaya

“…The expression of the ica gene is also regulated by various genes such as sigma B (sigB), staphylococcal accessory regulator A (SarA), and accessory gene regulator (agr). These genes interact through complex signaling mechanisms for biofilm formation (Serray et al 2016).…”

Verificação Da Temperatura De Distribuição De Refeições Quentes Ofertadas Em Uma Instituição De Longa Permanência Para Idosos E a Correlação Com O Crescimento Microbiológico

“…A TED só foi identificada em 2002 em uma amostra clínica de S. aureus(OLIVEIRA, D.;BORGES;SIMOES, 2018). Estudos indicam que a formação do biofilme é mediada pelo operon icaADBC, presente na maioria das linhagens de MRSA e MSSA(MONTEIRO et al, 2019;SERRAY et al, 2016). Este locus está envolvido com a produção da adesina intercelular polissacarídica (PIA) presente na matriz extracelular de S. aureus.…”