Biofilm formation and microscopic analysis of biofilms formed by Listeria monocytogenes in a food processing context

Kocot, Aleksandra Maria; Olszewska, Magdalena A.

doi:10.1016/j.lwt.2017.05.042

Cited by 54 publications

(39 citation statements)

References 120 publications

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“…Listeria sp. are microorganisms of particular interest in this topic because their great ability to produce biofilms and protect themselves from the action of disinfectants [16,49].…”

Section: Discussionmentioning

confidence: 99%

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

et al. 2019

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The Brazilian state of Mato Grosso is the largest producer and exporter of beef in the country, but few studies of relevance have been conducted to evaluate the microbiological safety of its products. This study aimed to estimate the prevalence of Listeria monocytogenes (LM) in export-approved beef from Mato Grosso and to characterize the isolates in terms of molecular properties and antimicrobial resistance. From a total of 50 samples analyzed, Listeria sp. was isolated in 18 (36% prevalence). Listeria monocytogenes was confirmed in 6 (12% prevalence). Among the serotype groups assessed by multiplex PCR, serotype 4 (4b, 4d or 4e) was the most prevalent. Although antibiotic resistance was not an issue, two strains isolated from different plants showed high resistance to sodium hypochlorite. Overall, this scenario causes concern because it puts at risk not only the Brazilian customer, but also the population of countries that import beef from Mato Grosso.

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“…Listeria sp. are microorganisms of particular interest in this topic because their great ability to produce biofilms and protect themselves from the action of disinfectants [16,49].…”

Section: Discussionmentioning

confidence: 99%

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

et al. 2019

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“…Listeria is known to produce thin biofilms by itself, although very different structures have been described depending on the growing conditions ( Rieu et al, 2008 ; Cherifi et al, 2017 ; Kocot and Olszewska, 2017 ). However, in the food industry, as in real environments in general, multispecies biofilms are prevalent ( Elias and Banin, 2012 ; Burmølle et al, 2014 ; Jahid and Ha, 2014 ; Giaouris et al, 2015 ; Sanchez-Vizuete et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Listeria monocytogenes Colonizes Pseudomonas fluorescens Biofilms and Induces Matrix Over-Production

et al. 2018

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In food facilities, biofilms or their debris might act as helpers for attracting free floating microorganisms. In this sense, Pseudomonas fluorescens, a dense biofilm producer frequently isolated from food contact surfaces, could be a good candidate for sheltering other microorganisms, such as Listeria monocytogenes. The main objective of this work was to evaluate the ability of L. monocytogenes to colonize pre-established Pseudomonas biofilms. For this, the movement throughout mature Pseudomonas biofilms of a green fluorescent protein (GFP) – tagged strain of L. monocytogenes was tracked for 24 h by confocal laser scanning microscopy (CLSM). Moreover, in order to check the effect of the incorporation of Listeria on the overall matrix production, attached populations of both microorganisms and total biomass (cells + matrix) of the resulting biofilms were measured over time. Planktonic cells of L. monocytogenes efficiently migrated to preformed P. fluorescens biofilms. Moreover, they moved preferentially toward the bottom layers of these structures, suggesting some kind of tropism. When preformed P. fluorescens biofilms were conditioning the surfaces, the L. monocytogenes attached population was on average, 1–2 Log higher than when this organism grew on bare coupons. Furthermore, the arrival of L. monocytogenes to the already established P. fluorescens biofilms led to a matrix over-production. Indeed, biomass values [optical density (OD595 nm)] of the resulting biofilms were double those of the ordinary L. monocytogenes–P. fluorescens mixed biofilms (1.40 vs. 0.6). The fact that L. monocytogenes cells accumulate in the bottom layers of preformed biofilms provides this microorganism an extra protection toward physical–chemical damages. This might partly explain why this microorganism can persist in food industry environments.

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“…L. monocytogenes is able to attach to food processing surfaces and multiply to form biofilms in inaccessible locations in processing facilities [9]. Biofilms protect the bacterium against antimicrobial action, enabling it to colonize food processing equipment, conveyor belts, pipes, floors and drainage systems and to persist for months or even years, cross-contaminating different surfaces in food processing plants [26].…”

Section: The Occurrence Of L Monocytogenes In the Food Processing Enmentioning

confidence: 99%

“…Hand tearing or manual slicing with a razor blade reduced the survival and growth of E. coli and L. innocua, probably because of less damage to the vegetable tissues and minimal leakage of nutrients from damaged plant tissues [8]. L. monocytogenes has the ability to attach itself to food preparation contact surfaces and grow to form protective biofilms, which generally protect the bacterial cells from antimicrobial action during cleaning and sterilisation processes [9]. However, low concentrations (<10 μg/mL) of paenibacterin have been found to suppress the growth of L. monocytogenes within the biofilm matrix as well as to down-regulate the genes involved in biofilm formation [10].…”

Section: Introductionmentioning

confidence: 99%

Contamination, Prevention and Control of Listeria monocytogenes in Food Processing and Food Service Environments

Tabit¹

2018

Listeria Monocytogenes

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This chapter reviews issues related to the occurrence and growth of Listeria monocytogenes in food processing and food service environments. L. monocytogenes is a food-borne pathogen with the capacity to contaminate raw or minimally processed foods such as chilled ready-to-eat (RTE) foods. The consumption of food contaminated with L. monocytogenes can result in a disease known as listeriosis among vulnerable groups of people such as pregnant women and fetuses, newborns, adults between the ages of 65 and 75, and people with weakened immune systems. L. monocytogenes is ubiquitous and has been isolated from soil, vegetation, sewage, water, animal feed, fresh and frozen meat including poultry, slaughterhouse wastes and the feces of healthy animals and humans. The bacterium is both acid tolerant and salt tolerant. It is able to grow at refrigerator temperature, and is therefore often associated with the consumption of raw or minimally processed and often chilled RTE foods. L. monocytogenes is able to form biofilms on food processing and preparation surfaces, which protects it from antimicrobial action. Continuous education of vulnerable groups regarding food safety will increase their awareness of the importance of practicing safer food handling practices such as hand washing and safe storage of RTE foods as a means to prevent listeriosis.

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Biofilm formation and microscopic analysis of biofilms formed by Listeria monocytogenes in a food processing context

Cited by 54 publications

References 120 publications

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

Listeria monocytogenes Colonizes Pseudomonas fluorescens Biofilms and Induces Matrix Over-Production

Contamination, Prevention and Control of Listeria monocytogenes in Food Processing and Food Service Environments

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