Biofilm formation and resistance to bactericides of Pseudomonas syringae pv. theae

Abstract

Biofilm-grown cells of Pseudomonas syringae pv. theae (P.s.theae) wild-type strain K9301 on abiotic surface had remarkable resistance to kasugamycin in comparison to planktonically grown cells; however, the biofilm-grown cells of K9301 had the same sensitivity to copper sulfate. Because both the lesser biofilm-forming strain K9301S3 and enhanced biofilm-forming strain K9301-6 also had remarkable biofilm resistance to kasugamycin just as K9301 did and because epigallocatechin gallate, which enhanced biofilm formation of P.s.theae, had no effect on biofilm resistance to kasugamaycin, the degree of biofilm formation was not correlated with the antibiotic susceptibilities. In addition, K9301 and K9301S3 had less sensitivity to kasugamycin but had high sensitivity to copper sulfate on nonwounded leaf surfaces. These results indicate a possibility that the mechanism of P.s.theae biofilm resistance to bactericide functions on both abiotic and nonwounded leaf surfaces.

Biofilm-Forming Ability of Phytopathogenic Bacteria: A Review of its Involvement in Plant Stress

“…Many phytopathogenic bacteria produce biofilm on the leaf surface, such as P. syringae pv. theae, which is able to survive drought by living within biofilms on tea leaves [105]. Other Pseudomonas spp., such as P. aeruginosa on Arabidopsis taliana and P. syringae pv.…”

Nanocomposite of Ag-Doped ZnO and AgO Nanocrystals as a Preventive Measure to Control Biofilm Formation in Eggshell and Salmonella spp. Entry Into Eggs

“…Biofilm is the aggregation of sessile microorganisms on a surface, followed by self-production of extracellular matrix, composed of protein and exopolysaccharides (Donlan and Costerton, 2002; Martínez and Vadyvaloo, 2014). This mechanism confers to the bacteria capacities of persistence and survival in adverse environments, and especially, resistance to the action of biocides, since this physical barrier hinders the action of the disinfectant agent (Nikolaev and Plakunov, 2007; Tomihama et al, 2007; Flemming and Wingender, 2010). The formation of biofilms is a great concern given their ability to remain in environments, resisting sanitizers and antimicrobials, and give rise to a constant source of microorganisms, favoring pathogen propagation in production chains and, consequently, in foods (Schlisselberg and Yaron, 2013).…”

Formação de biofilmes e produção de moléculas sinalizadoras de quorum sensing por cepas de Salmonellla spp. isoladas de processamento de frango

“…Células estruturadas em um biofilme apresentam maior resistência à ação de sanitizantes e antimicrobianos do que as células planctônicas em suspensão (NIKOLAEV, PLAKUNOV, 2007;TOMIHAMA, NISHI, ARAI, 2007). Esta resistência é devido à diminuição da difusão dos agentes sanitizantes através da complexa estrutura de EPS, presença de enzimas que degradam as substâncias antimicrobianas e alterações no meio (pH, presença de matéria orgânica) que afetam a atividade dos sanitizantes.…”

“…As fotomicrografias de superfícies de cupons de aço inoxidável, submetidas ao processo de adesão e formação de biofilme por sorotipos de Salmonella em As células de Vibrio harveyi BB170, utilizadas como biossensor neste trabalho, apresentam uma mutação no gene sensor para AI-1 (autoindutor 1) e produzem bioluminescência exclusivamente na presença de AI-2. As células de Vibrio harveyi BB170 são também capazes de produzir o autoindutor AI-2, pois o gene luxs continua intacto (TAGA, XAVIER, 2011). Durante os ensaios, à medida que a concentração de AI-2 exógeno, presente nos sobrenadante do cultivo de Salmonella diminui, devido à degradação das moléculas, a produção de bioluminescência também diminui, geralmente nas primeiras horas.…”

“…Considerando este fato de que as células de Vibrio harveyi BB170 também produzem AI-2, a detecção deste autoindutor exógeno deve ser registrada considerando o período imediatamente anterior à produção de AI-2 endógeno, pois mais tarde, geralmente após 7 ou 8 horas, todas as amostras apresentam bioluminescência (TAGA, XAVIER, 2011).…”