Biofilm formation by bacterial isolates from patients on indwelling medical devices

Gogoi, M.P.; Sharma, Akhilesh; Hazarika, Naba Kumar

doi:10.4103/0255-0857.154896

Cited by 12 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…evaluated of late by other authors, which is time consuming and expensive. 21,22 A study by Mathur et al 23 the TCP assay, only 7(4.6 %) of 152 tested Staphylococcus spp isolates displayed a biofilm positive phenotype, in contrast to our study where 49(61.25%) isolates were biofilm positive. The TM correlates well with the TCP test for strongly biofilm producing isolates but it was difficult to discriminate between weak and biofilm negative isolates due to the variability in observed results by different observers.…”

Section: Biofilm Formation Tcp (%) Tm (%) Cra (%)contrasting

confidence: 88%

Analysing Three Different Screening Methods for Biofilm Formation in Clinical Isolates of Candida

Khatri¹,

Sumana²,

Mahale³

et al. 2015

jemds

View full text Add to dashboard Cite

Biofilm formation helps the microbes to escape host defenses and develop resistance against antimicrobial agents. Early detection of slime production by the Candida species may direct the clinical management. The study was taken with the aim to estimate biofilm formation by Candida species in indwelling catheters and to compare three methods i.e. Congo red agar (CRA) method, Tissue culture plate (TCP) method, and tube method (TM). In this study, we evaluated the reliability of these methods in order to determine most suitable screening method and noted the sensitivity pattern of these isolates with the help of Vitek-2. Our data indicates that the TCP is an accurate and reproducible method for screening and can serve as a reliable quantitative tool for determining bio film formation by clinical isolates of Candida species along with susceptibility testing to reduce resistance pattern.

show abstract

Section: Biofilm Formation Tcp (%) Tm (%) Cra (%)contrasting

confidence: 88%

Analysing Three Different Screening Methods for Biofilm Formation in Clinical Isolates of Candida

Khatri¹,

Sumana²,

Mahale³

et al. 2015

jemds

View full text Add to dashboard Cite

show abstract

“…As such, inhibition of biofilm formation has a great impact on reduction of bacterial resistance [2,11,14]. P. aeruginosa is known to establish persistent biofilms on indwelling medical devices [15]. It employs an efficient collection of enzymes and other virulence factors to attack and infect a wide diversity of human tissues [16].…”

Section: Introductionmentioning

confidence: 99%

Tackling Virulence of Pseudomonas aeruginosa by the Natural Furanone Sotolon

et al. 2021

View full text Add to dashboard Cite

The bacterial resistance development due to the incessant administration of antibiotics has led to difficulty in their treatment. Natural adjuvant compounds can be co-administered to hinder the pathogenesis of resistant bacteria. Sotolon is the prevailing aromatic compound that gives fenugreek its typical smell. In the current work, the anti-virulence activities of sotolon on Pseudomonas aeruginosa have been evaluated. P. aeruginosa has been treated with sotolon at sub-minimum inhibitory concentration (MIC), and production of biofilm and other virulence factors were assessed. Moreover, the anti-quorum sensing (QS) activity of sotolon was in-silico evaluated by evaluating the affinity of sotolon to bind to QS receptors, and the expression of QS genes was measured in the presence of sotolon sub-MIC. Furthermore, the sotolon in-vivo capability to protect mice against P. aeruginosa was assessed. Significantly, sotolon decreased the production of bacterial biofilm and virulence factors, the expression of QS genes, and protected mice from P. aeruginosa. Conclusively, the plant natural substance sotolon attenuated the pathogenicity of P. aeruginosa, locating it as a plausible potential therapeutic agent for the treatment of its infections. Sotolon can be used in the treatment of bacterial infections as an alternative or adjuvant to antibiotics to combat their high resistance to antibiotics.

show abstract

“…Karen smith et al, (2008) has documented biofilm production to be 53.8% among the MRSA. (2) Gogoi et al,(2015) has documented 50% of MRSA as biofilm producers.…”

Section: Discussionmentioning

confidence: 99%

“…www.ijbio.com 4994 (17) MRSA are multidrug resistant isolates and along with biofilm producing phenotype becomes a greater therapeutic challenge. Dadri kaur et al, (2014) has documented 70 (82.3%) as biofilm producers among the MRSA.…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation and Antibiotic susceptibility pattern among Staphylococcus aureus in a tertiary care hospital in Kanchipuram: An Evaluation of screening methods for biofilm formation

Jayachandran¹,

Sarasa²,

Doris³

et al. 2016

IJB

View full text Add to dashboard Cite

The ability of Staphylococcus aureus to form biofilms is of significant clinical interest, as biofilm development impacts the efficacy of antimicrobial therapy and the subsequent outcome of an infection. The present study is undertaken to detect the biofilm production and to determine the antibiotic susceptibility pattern among the Staphylococcus aureus isolates. A total of 100 Staphylococcus aureus isolated for the first time from pus, blood, catheter, IV cannulas were included in the study. Biofilm detection was done by tube method and Microtitre plate method. Antibiotic susceptibility was done by Kirby bauer disc diffusion method. Methicillin resistance was detected by Cefoxitin disc diffusion method. By tube method and Microtitre plate method 26% and 46% of the isolates were identified as biofilm producers. By Microtitre plate method, BHI broth (Brain heart infusion broth) and BHI broth with sucrose was used and the difference in the biofilm forming ability was compared. When BHI broth with sucrose was used 69% showed biofilm formation whereas when tested with BHI broth, only 46% were identified as biofilm producers. Good sensitivity was observed for Amikacin (88%) and cefotaxime (82%). MRSA (Methicillin resistant Staphylococcus aureus) was detected among 19% of the isolates. Among the biofilm producers if there are drug resistant bacteria like MRSA the problem becomes challenging and requires combination of several antibiotics. Hence Screening for biofilm production by bacterial isolates should be performed. Infection control program should address the effective execution of disinfection procedures.

show abstract

Biofilm formation by bacterial isolates from patients on indwelling medical devices

Cited by 12 publications

References 5 publications

Analysing Three Different Screening Methods for Biofilm Formation in Clinical Isolates of Candida

Analysing Three Different Screening Methods for Biofilm Formation in Clinical Isolates of Candida

Tackling Virulence of Pseudomonas aeruginosa by the Natural Furanone Sotolon

Biofilm formation and Antibiotic susceptibility pattern among Staphylococcus aureus in a tertiary care hospital in Kanchipuram: An Evaluation of screening methods for biofilm formation

Contact Info

Product

Resources

About