Biofilm formation by coagulase-negative staphylococci: Impact on the efficacy of antimicrobials and disinfectants commonly used on dairy farms

Tremblay, Yannick D. N.; Caron, Vincent; Blondeau, Andréanne; Messier, Serge; Jacques, Mario

doi:10.1016/j.vetmic.2014.06.007

Cited by 45 publications

(34 citation statements)

References 27 publications

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“…CNS are commonly found in food, environment, and clinical setting (Huber et al 2011 ), and were recently recognized as etiologic agents of animal and human infectious diseases (Tremblay et al 2014 ). Antimicrobial resistance is an important virulence factor (Wang et al 2006 ), but little information is available on the prevalence of antimicrobial resistance in CNS from leaf vegetables.…”

Section: Resultsmentioning

confidence: 99%

Improved multiplex PCR primers for rapid identification of coagulase-negative staphylococci

et al. 2017

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Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified as S. capitis, S. caprae, S. epidermidis, S. haemolyticus, S. pasteuri, S. saprophyticus, S. warneri, and S. xylosus. Although 16S rRNA sequencing is universally accepted as a standard for bacterial identification, the method did not effectively discriminate closely related species, and additional DNA sequencing was required. The divergence of the sodA gene sequence is higher than that of 16S rRNA. To devise a rapid and accurate identification method, sodA-specific primers were designed to demonstrate that species-specific multiplex polymerase chain reaction (PCR) can be used for the identification of CNS species. The accuracy of this method was higher than that of phenotypic identification; the method is simple and less time-consuming than 16S rRNA sequencing. Of the 55 CNS isolates, 92.72% were resistant to at least one antibiotic, and 60% were resistant to three or more antibiotics. CNS isolates produced diverse virulence-associated enzymes, including hemolysin (produced by 69.09% of the isolates), protease (65.45%), lipase (54.54%), lecithinase (36.36%), and DNase (29.09%); all isolates could form a biofilm. Because of the increasing pathogenic significance of CNS, the efficient multiplex PCR detection method developed in this study may contribute to studies for human health.Electronic supplementary materialThe online version of this article (doi:10.1007/s00203-017-1415-9) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Improved multiplex PCR primers for rapid identification of coagulase-negative staphylococci

et al. 2017

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“…The plates were incubated overnight at 37°C, and colonies on the plates then were counted to determine the number of STEC cells present. In addition to CFU, the metabolic activity of the treated biofilms also was evaluated using the CellTiter-Blue reagent (Promega Corporation) as described previously (33). CellTiter-Blue reagent is a resazurin solution that is reduced by viable cells into resorufin, which emits fluorescence.…”

Section: Methodsmentioning

confidence: 99%

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Vogeleer

Tremblay

Jubelin³

et al. 2016

Appl Environ Microbiol

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bForming biofilms may be a survival strategy of Shiga toxin-producing Escherichia coli to enable it to persist in the environment and the food industry. Here, we evaluate and characterize the biofilm-forming ability of 39 isolates of Shiga toxin-producing Escherichia coli isolates recovered from human infection and belonging to seropathotypes A, B, or C. The presence and/or production of biofilm factors such as curli, cellulose, autotransporter, and fimbriae were investigated. The polymeric matrix of these biofilms was analyzed by confocal microscopy and by enzymatic digestion. Cell viability and matrix integrity were examined after sanitizer treatments. Isolates of the seropathotype A (O157:H7 and O157:NM), which have the highest relative incidence of human infection, had a greater ability to form biofilms than isolates of seropathotype B or C. Seropathotype A isolates were unique in their ability to produce cellulose and poly-N-acetylglucosamine. The integrity of the biofilms was dependent on proteins. Two autotransporter genes, ehaB and espP, and two fimbrial genes, z1538 and lpf2, were identified as potential genetic determinants for biofilm formation. Interestingly, the ability of several isolates from seropathotype A to form biofilms was associated with their ability to agglutinate yeast in a mannose-independent manner. We consider this an unidentified biofilmassociated factor produced by those isolates. Treatment with sanitizers reduced the viability of Shiga toxin-producing Escherichia coli but did not completely remove the biofilm matrix. Overall, our data indicate that biofilm formation could contribute to the persistence of Shiga toxin-producing Escherichia coli and specifically seropathotype A isolates in the environment. Shiga toxin-producing Escherichia coli (STEC) strains, including enterohemorrhagic E. coli (EHEC), are food-borne and waterborne human enteric pathogens responsible for infections. They are associated with important public health concerns in developed countries. Symptoms associated with STEC infections range from abdominal cramps and bloody diarrhea to postinfectious complications, such as hemolytic-uremic syndrome (HUS). HUS, a life-threatening complication of STEC infections, is a consequence of Shiga toxin production. According to the Public Health Agency of Canada (PHAC), more than 30,000 cases of STEC infections occur each year in Canada (1). Environmentally, the main reservoir for STEC is cattle. Transmission of STEC to humans typically is associated with the consumption of contaminated food such as undercooked beef, fresh produce, unpasteurized milk, or contaminated drinking water (2), but these pathogens also can be transmitted from person to person or via direct contact with animals or their feces.The 2003 Karmali seropathotype model classifies STEC into seropathotypes based on their reported incidence in human disease, outbreaks, and/or association with the development of severe symptoms in humans (3). Serotypes frequently responsible for hemorrhagic colitis (HC) and HUS, ...

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“…Biofilm formation. Biofilms have been associated with adherence to biotic and abiotic surfaces, tolerance to antimicrobials and persistence into the environment [55]. Thus, this analysis was carried out to understand if the recovered MRSA of meat origin were biofilm producers.…”

Section: Plasmid and Southern Blot Hybridization Analysismentioning

confidence: 99%

Prevalence and characteristics of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) isolated from chicken meat in the province of Quebec, Canada

et al. 2020

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This study was conducted to estimate the prevalence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) in retail chicken meat and broiler chickens from the Province of Quebec, Canada, and to characterize LA-MRSA isolates. A total of 309 chicken drumsticks and thighs were randomly selected in 2013 from 43 retail stores in the Monteregie. In addition, nasal swabs and caeca samples were collected in 2013-2014 from 200 broiler chickens of 38 different flocks. LA-MRSA was not detected in broiler chickens. Fifteen LA-MRSA isolates were recovered from four (1.3%) of the 309 chicken meat samples. Multi-Locus Sequence Typing (MLST) and SCCmec typing revealed two profiles (ST398-MRSA-V and ST8-MRSA-IVa), which were distinct using pulse-field gel electrophoresis (PFGE) and microarray (antimicrobial resistance and virulence genes) analyses. In addition to beta-lactam resistance, tetracycline and spectinomycin resistance was detected in all isolates from the 3 positive samples of the ST398 profile. Southern blot hybridization revealed that the resistance genes aad(D) and lnu(A), encoding resistances to aminoglycosides and lincosamides respectively, were located on plasmid. All isolates were able to produce biofilms, but biofilm production was not correlated with hld gene expression. Our results show the presence of two separate lineages of MRSA in retail chicken meat in Quebec, one of which is likely of human origin.

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Biofilm formation by coagulase-negative staphylococci: Impact on the efficacy of antimicrobials and disinfectants commonly used on dairy farms

Cited by 45 publications

References 27 publications

Improved multiplex PCR primers for rapid identification of coagulase-negative staphylococci

Improved multiplex PCR primers for rapid identification of coagulase-negative staphylococci

Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections

Prevalence and characteristics of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) isolated from chicken meat in the province of Quebec, Canada

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