Biofilm formation by<i>Candida albicans</i>and<i>Streptococcus mutans</i>in the presence of farnesol: a quantitative evaluation

Fernandes, Rosa Áurea Quintella; Monteiro, Douglas Roberto; Arias, Laís Salomão; Fernandes, Gabriela Lopes; Delbem, Alberto Carlos Botazzo; Barbosa, Débora de Barros

doi:10.1080/08927014.2016.1144053

Cited by 71 publications

(43 citation statements)

References 43 publications

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“…This molecule is a key regulator of C. albicans biofilm formation [63], and has also been shown to augment antimicrobial therapy and decrease bacterial biofilm formation [64,65]. These effects are also observed with S. mutans, with high concentrations of the compound inhibiting biofilm formation [66,67]. Yet, interestingly, when at lower concentrations (25-50 μM) that are representative within these dualspecies biofilms, then it can stimulate S. mutans microcolony development and enhance biofilm formation [67].…”

Section: Caries: Slimy Residentsmentioning

confidence: 96%

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Delaney

Kean

Short

et al. 2018

Curr Clin Micro Rpt

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Purpose of Review Over the last decade, microbiome studies have enhanced our knowledge and understanding of the polymicrobial nature of oral infections. Recently, profiling of the fungal microbiome has expanded our conventional understanding of oral ecology, revealing the critical importance of yeasts within this complex microbiome. This review aims to explore our current appreciation of interkingdom interactions in oral disease. Recent Findings There is a growing evidence base of interactions and pathogenic synergy and antagonism with bacterial species within oral disease. Recent studies have helped to develop our knowledge of how Candida albicans, alongside bacteria such as Porphyromonas gingivalis, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Lactobacillus species, influence overall pathogenicity. Summary Clinical and experimental evidence makes a compelling case for a role for C. albicans in a number of oral infections, though whether its role is an active accomplice or passive bystander remains to be determined.

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Section: Caries: Slimy Residentsmentioning

confidence: 96%

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Delaney

Kean

Short

et al. 2018

Curr Clin Micro Rpt

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“…Then, farnesol was diluted in AS and added to the wells at the following concentrations: 0.78 and 1.56 mM (corresponding to 1/8 and 1/4 of the minimum inhibitory concentration (MIC) determined for planktonic cells) for assessment of the effect on acid production and enzymatic activity; 3.12 mM, for the time-kill curve assay; 3.12 and 12.5 mM for evaluation of the effect on the extracellular matrix composition and the structure of the biofilms. Afterwards, the plates were stored at 37°C in 5% CO 2 for 48 h, and the media were renewed after 24 h. All farnesol concentrations were based on the Fernandes et al [22] study. Subinhibitory concentrations (0.78 and 1.56 mM) were used to ensure that the results were not influenced by the death of microorganisms, whereas the inhibitory concentrations (3.12 and 12.5 mM) were applied aiming for biofilm eradication.…”

Section: Single and Mixed Biofilm Formation In The Presence Of Farnesolmentioning

confidence: 99%

“…Absorbance values were correlated with the concentration of proteins and carbohydrates, and the results were expressed as a function of the biofilm dry weight (mg/g dry weight). CHG at 0.37 mM (509 C. albicans MIC) [22] was used as positive control, and AS devoid of farnesol was used as negative control.…”

Section: Effect Of Farnesol On Matrix Composition Of Biofilmsmentioning

confidence: 99%

Virulence Factors in Candida albicans and Streptococcus mutans Biofilms Mediated by Farnesol

et al. 2018

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The aim of this study was to evaluate the effect of farnesol on the production of acids and hydrolytic enzymes by biofilms of and. The present study also evaluated the time-kill curve and the effect of farnesol on matrix composition and structure of single-species and dual-species biofilms. Farnesol, at subinhibitory concentrations, showed a significant reduction in biofilm acid production, but did not alter hydrolytic enzyme production. The number of cultivable cells of both microorganisms was significantly reduced after 8 h of contact with farnesol. Extracellular matrix protein content was reduced for biofilms formed in the presence of farnesol. In addition, confocal laser scanning and scanning electron microscopy displayed structural alterations in all biofilms treated with farnesol, which included reduction in viable cells and extracellular matrix. In conclusion, farnesol showed favorable properties controlling some virulence factors of and biofilms. These findings should stimulate further studies using this quorum-sensing molecule, combined with other drugs, to prevent or treat biofilm-associated oral diseases.

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“…Although farnesol caused inhibition of biofilm formed by single C. albicans species, as previously reported [41,42], it did not reduce the biofilm formed by mixed species. The presence of bacteria is known to increase the resistance of such biofilms [22].…”

Section: Discussionmentioning

confidence: 99%

Optimum Inhibition of Amphotericin-B-Resistant Candida albicans Strain in Single- and Mixed-Species Biofilms by Candida and Non-Candida Terpenoids

et al. 2020

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Candida albicans is one of the most common human fungal pathogens and represents the most important cause of opportunistic mycoses worldwide. Surgical devices including catheters are easily contaminated with C. albicans via its formation of drug-resistant biofilms. In this study, amphotericin-B-resistant C. albicans strains were isolated from surgical devices at an intensive care center. The objective of this study was to develop optimized effective inhibitory treatment of resistant C. albicans by terpenoids, known to be produced naturally as protective signals. Endogenously produced farnesol by C. albicans yeast and plant terpenoids, carvacrol, and cuminaldehyde were tested separately or in combination on amphotericin-B-resistant C. albicans in either single- or mixed-infections. The results showed that farnesol did not inhibit hyphae formation when associated with bacteria. Carvacrol and cuminaldehyde showed variable inhibitory effects on C. albicans yeast compared to hyphae formation. A combination of farnesol with carvacrol showed synergistic inhibitory activities not only on C. albicans yeast and hyphae, but also on biofilms formed from single- and mixed-species and at reduced doses. The combined terpenoids also showed biofilm-penetration capability. The aforementioned terpenoid combination will not only be useful in the treatment of different resistant Candida forms, but also in the safe prevention of biofilm formation.

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Biofilm formation byCandida albicansandStreptococcus mutansin the presence of farnesol: a quantitative evaluation

Cited by 71 publications

References 43 publications

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Virulence Factors in Candida albicans and Streptococcus mutans Biofilms Mediated by Farnesol

Optimum Inhibition of Amphotericin-B-Resistant Candida albicans Strain in Single- and Mixed-Species Biofilms by Candida and Non-Candida Terpenoids

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