Biofilm Formation by<i>Pseudomonas</i>spp. and Their Significance as a Biocontrol Agent

Siddiqui, Zaki A.; Khan, M. Masroor A.

doi:10.1002/9781119246329.ch5

Cited by 6 publications

(3 citation statements)

References 163 publications

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“…The production of hydrolytic enzymes by PGPR is an essential mechanism against plant pathogens for sustainable plant disease management. These enzymes break down the cell walls of fungal pathogens, causing cell death [ 7 ]. Plant growth-promoting bacteria produce hydrolytic enzymes (chitinase, glucanase, protease, and cellulase) which are responsible for the lysis of phytopathogens through hyperparasitism.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the production of antibiotics, antagonistic bacterial isolate s also produce extracellular enzymes [ 5 ]. Extracellular enzymes produced by various microorganisms are used as hydrolytic enzymes, degrading the cell wall structural components of most fungi [ 6 , 7 ]. Extracellular enzymes are required for the degradation of the fungal pathogen mycelia [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Evaluation of Extracellular Enzyme Activity and Its Biocontrol Efficacy of Bacterial Isolates from Pepper Plants for the Management of Phytophthora capsici

Admassie

Wolde-Hawariat

Alemu

2022

BioMed Research International

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Phytophthora capsici is one of the most devastating fungal pathogens, causing severe diseases that lead to economic loss in the pepper industry. As a result of the infections, the chemical approach is becoming more popular. Biological control, on the other hand, is better suited to controlling fungal pathogens. The biological control approach significantly reduces the problems associated with chemical applications while restoring natural environmental balance. As a result, the overall findings indicate that certain bacterial isolates play a beneficial role in lytic enzyme production and biocontrol activities against P. capsici. Bacterial isolates obtained from the pepper plants were screened for lytic enzyme and anti-oomycete activity against Phytophthora capsici in Ethiopia. Sixty bacterial isolates were isolated and tested against Phytophthora capsici. From these bacterial isolates, different inhibition zones and hydrolytic enzyme production were detected. Biochemical tests using an automated machine (MALDI-TOF, VITEK 2 compact and 16S rRNA) revealed that three of them, AAUSR23, AAULE41, and AAULE51, showed a high inhibition zone and high production of hydrolytic enzymes and were identified as Enterobacter cloacae (AAUSR23), Pseudomonas fluorescens (AAULE41), and undetermined (AAULE51). The effects of diffusable metabolite isolate AAULE51 has a 66.7% inhibition zone against Phytophthora capsici, followed by AAULE41 and AAUSR23, which have 59.7% and 14.1% inhibition zones, respectively. These bacterial isolates showed high production of hydrolytic enzymes like protease, cellulase, chitinase, and lipase (5-34 diameter of inhibition zone). As a result, the overall findings show that selected bacterial isolates play a beneficial role in lytic enzyme production and for their biocontrol activities against P. capsici.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Extracellular Enzyme Activity and Its Biocontrol Efficacy of Bacterial Isolates from Pepper Plants for the Management of Phytophthora capsici

Admassie

Wolde-Hawariat

Alemu

2022

BioMed Research International

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“…Klebsiella sp., Pseudomonas sp. Isolates and Streptomyces hygroscopicus proved to be potential bioagents against many fungi like Aspergillus niger, A. flavus, Fusarium oxysporum (Stockwell and Stack, 2007;Siddiqui and Khan, 2017). The inhibitory efficacy of target bacterial culture filtrate was assessed in vitro and showed moderate inhibitory effect on leaf lesion size at highest concentration.…”

Section: Effect Of Bacterial Isolates Against P Infestans In Detachementioning

confidence: 99%

Efficacy of Bacterial Isolates against Causal Agent of Late Blight of Potato, Phytophthora infestans

Rani

Upadhyay

Shukla

et al. 2020

IJARe

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Background: Potato (Solanum tuberosum) is main crop in subtropical and temperate regions as vegetable crop. It is affected by Phytophthora infestans causing a devasting disease late blight. The chemical pesticides used against this disease in this present scenario cause pollution (soil as well as water) and adversely affect beneficial soil micro-flora, grazing animals and human beings. Methods: An experiment was designed at the Department of Botany, K.V. Subharti College of Science, Swami Vivekanand Subharti University, Meerut (UP), India during 2017-2018. Three bacterial bioagents, Pseudomonas isolate 1 and 2 and Bacillus species was isolated from infected phylloplane and used against late blight pathogen, P. infestans. The efficacy of antagonistic bio-agents tested against mycelial growth, zoospore and zoosporangial germination of P. infestans by dual culture method and detached leaf method.Result: The present study focused on use of bio-agents or antagonistic bacteria to control late blight pathogen showed concentration biased potential inhibitory activity against mycelial growth, zoospore and zoosporangial germination. It means higher the concentration of bacterial isolate, greater the inhibitory effect on various tested parameters. Among the three tested antagonistic bioagents, Bacillus showed maximum zone of inhibition to late blight pathogen. The proved that the Pseudomaonas and Bacillus as potential antagonistic agent for ecofriendly sustainable management of pathogenic mycoflora, P. infestans.

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Extracellular Polymeric Substances from Agriculturally Important Microorganisms

et al. 2021

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Biofilm Formation byPseudomonasspp. and Their Significance as a Biocontrol Agent

Cited by 6 publications

References 163 publications

In Vitro Evaluation of Extracellular Enzyme Activity and Its Biocontrol Efficacy of Bacterial Isolates from Pepper Plants for the Management of Phytophthora capsici

In Vitro Evaluation of Extracellular Enzyme Activity and Its Biocontrol Efficacy of Bacterial Isolates from Pepper Plants for the Management of Phytophthora capsici

Efficacy of Bacterial Isolates against Causal Agent of Late Blight of Potato, Phytophthora infestans

Extracellular Polymeric Substances from Agriculturally Important Microorganisms

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